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Employing monospecific antibodies and laser confocal microscopy, we studied the localization of the receptor protein in the human and wild-type mouse ductuli efferentes and epididymis. We show that HE6\/GPR64 is specifically associated with cell types and subcellular domains involved in the process of fluid reabsorption. In the mouse, dual labelling with anti-tubulin antibodies revealed that HE6\/GPR64 was absent from the (kino-) cilia of ciliated cells. Instead, the receptor protein accumulated in the non-ciliated principal cells. Specifically, strong immunofluorescence was observed in the apical compartment of these cells. Dual labelling with phalloidin and anti-ezrin antibodies revealed that in the mouse the bulk amount of HE6\/GPR64 protein co-localized with the F-actin\u2013ezrin scaffold in brush border-like microvilli of ductuli efferentes and long stereocilia of the epididymis proper. In the ductuli efferentes, HE6\/GPR64 also co-localized with the subapical F-actin network immediately below the microvilli. Comparable immunostaining patterns were observed in human and mouse; however, a specific feature of the human ductuli efferentes was an intense HE6\/GPR64-related labelling of crypt-like grooves or furrows of hitherto unknown function.<\/jats:p>","DOI":"10.1530\/rep-08-0078","type":"journal-article","created":{"date-parts":[[2008,5,9]],"date-time":"2008-05-09T20:45:04Z","timestamp":1210365904000},"page":"235-245","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":31,"title":["HE6\/GPR64 adhesion receptor co-localizes with apical and subapical F-actin scaffold in male excurrent duct epithelia"],"prefix":"10.1093","volume":"136","author":[{"given":"Christiane","family":"Kirchhoff","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Osterhoff","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annemarie","family":"Samalecos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2008,8,1]]},"reference":[{"key":"2026010712482017800_bib1","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/S0940-9602(99)80020-X","article-title":"Apocrine secretion \u2013 fact or artifact?","volume":"181","author":"Aum\u00fcller","year":"1999","journal-title":"Annals of Anatomy"},{"key":"2026010712482017800_bib2","doi-asserted-by":"crossref","first-page":"2960","DOI":"10.1210\/endo.139.6.6046","article-title":"Relaxin-like factor expression as a marker of differentiation in the mouse testis and ovary","volume":"139","author":"Balvers","year":"1998","journal-title":"Endocrinology"},{"key":"2026010712482017800_bib3","doi-asserted-by":"crossref","first-page":"1025","DOI":"10.1242\/jcs.105.4.1025","article-title":"Ezrin is concentrated in the apical microvilli of a wide variety of epithelial cells whereas moesin is found primarily in endothelial cells","volume":"105","author":"Berryman","year":"1993","journal-title":"Journal of Cell Science"},{"key":"2026010712482017800_bib4","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/BF01463929","article-title":"Antigen retrieval in cryostat tissue sections and cultured cells by treatment with sodium dodecyl sulfate (SDS)","volume":"105","author":"Brown","year":"1996","journal-title":"Histochemistry and Cell Biology"},{"key":"2026010712482017800_bib5","doi-asserted-by":"crossref","first-page":"F619","DOI":"10.1152\/ajprenal.2001.280.4.F619","article-title":"Altered membrane-cytoskeleton linkage and membrane blebbing in energy-depleted renal proximal tubular cells","volume":"280","author":"Chen","year":"2001","journal-title":"American Journal of Physiology. 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