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The survival rate of ejaculated spermatozoa incubated in hypotonic solutions ranging from 3 to 330\u200ammol\/kg followed a sigmoid curve that fitted a simple logistic model. The critical osmolality value (Osmcrit) at which 50% of spermatozoa died was determined with this model. Hypotonic resistance of spermatozoa increased with temperature between 15 and 39\u200a\u00b0C and decreased after hydrogen superoxide treatment, but was not modified during 8 days of preservation in Beltsville thawing solution. Hypotonic resistance markedly decreased during epididymal maturation and after ejaculation as Osmcrit at 15\u200a\u00b0C was 54.7\u00b13.2, 68.5\u00b110.6, 116.7\u00b12.1 and 194.3\u00b13.7\u200ammol\/kg for the caput, corpus, cauda and ejaculated spermatozoa respectively. Hypo-osmotic stress of 100\u200ammol\/kg revealed a sperm subpopulation exhibiting increased hypotonic resistance compared with the whole ejaculate (Osmcrit=67.8\u00b12.1\u200ammol\/kg). Consistent differences were observed between lean and standard breeds (Pietrain versus Large White) and between boars within the same breed. According to data collected by artificial insemination centers during a large-scale field trial, hypotonic resistance of ejaculates was found to be positively correlated with in vivo fertility.<\/jats:p>","DOI":"10.1530\/rep-08-0225","type":"journal-article","created":{"date-parts":[[2008,11,7]],"date-time":"2008-11-07T20:55:09Z","timestamp":1226091309000},"page":"205-213","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":35,"title":["Hypotonic resistance of boar spermatozoa: sperm subpopulations and relationship with epididymal maturation and fertility"],"prefix":"10.1093","volume":"137","author":[{"given":"Xavier","family":"Druart","sequence":"first","affiliation":[{"name":"Union Nationale des Coop\u00e9ratives d\u2019Elevage et d\u2019Ins\u00e9mination Animale 13 rue Jouet, 94704 Cedex Maisons Alfort, France 2UMR 6175 INRA CNRS-Universit\u00e9 de Tours-Haras Nationaux, Station de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, 37380 Nouzilly, France 3Unit\u00e9 de recherche Math\u00e9matiques et Informatique Appliqu\u00e9es INRA Domaine de Vilvert, 78352 Cedex Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Luc","family":"Gatti","sequence":"additional","affiliation":[{"name":"Union Nationale des Coop\u00e9ratives d\u2019Elevage et d\u2019Ins\u00e9mination Animale 13 rue Jouet, 94704 Cedex Maisons Alfort, France 2UMR 6175 INRA CNRS-Universit\u00e9 de Tours-Haras Nationaux, Station de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, 37380 Nouzilly, France 3Unit\u00e9 de recherche Math\u00e9matiques et Informatique Appliqu\u00e9es INRA Domaine de Vilvert, 78352 Cedex Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Huet","sequence":"additional","affiliation":[{"name":"Union Nationale des Coop\u00e9ratives d\u2019Elevage et d\u2019Ins\u00e9mination Animale 13 rue Jouet, 94704 Cedex Maisons Alfort, France 2UMR 6175 INRA CNRS-Universit\u00e9 de Tours-Haras Nationaux, Station de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, 37380 Nouzilly, France 3Unit\u00e9 de recherche Math\u00e9matiques et Informatique Appliqu\u00e9es INRA Domaine de Vilvert, 78352 Cedex Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Louis","family":"Dacheux","sequence":"additional","affiliation":[{"name":"Union Nationale des Coop\u00e9ratives d\u2019Elevage et d\u2019Ins\u00e9mination Animale 13 rue Jouet, 94704 Cedex Maisons Alfort, France 2UMR 6175 INRA CNRS-Universit\u00e9 de Tours-Haras Nationaux, Station de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, 37380 Nouzilly, France 3Unit\u00e9 de recherche Math\u00e9matiques et Informatique Appliqu\u00e9es INRA Domaine de Vilvert, 78352 Cedex Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrice","family":"Humblot","sequence":"additional","affiliation":[{"name":"Union Nationale des Coop\u00e9ratives d\u2019Elevage et d\u2019Ins\u00e9mination Animale 13 rue Jouet, 94704 Cedex Maisons Alfort, France 2UMR 6175 INRA CNRS-Universit\u00e9 de Tours-Haras Nationaux, Station de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, 37380 Nouzilly, France 3Unit\u00e9 de recherche Math\u00e9matiques et Informatique Appliqu\u00e9es INRA Domaine de Vilvert, 78352 Cedex Jouy-en-Josas, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,2,1]]},"reference":[{"key":"2026010712494703100_bib1","doi-asserted-by":"crossref","first-page":"458","DOI":"10.1016\/j.theriogenology.2004.09.046","article-title":"New assays for detection and 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