{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T13:02:31Z","timestamp":1770987751388,"version":"3.50.1"},"reference-count":76,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T00:00:00Z","timestamp":1246406400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Functionally immature spermatozoa leave the testis mature during epididymal transit. This process of maturation involves either addition of new proteins or modification of existing proteins onto the sperm domains that are responsible for domain-specific functions. Epididymal proteins are preferred targets for immunocontraception. In an attempt to identify epididymis-specific sperm proteins, we used a novel combinatorial approach comprising subtractive immunization (SI) followed by proteomics. Following SI, sera of mice were used for immunoproteomics, which led to the identification of 30 proteins, of which four proteins namely sperm head protein 1, sperm flagella protein 2 (SFP2), SFP3, and SFP4 are being reported for the first time on sperm. Another group of four proteins namely collagen \u03b1-2 (I) chain precursor, homeodomain-interacting protein kinase 1, GTP-binding protein Rab1, and ubiquinol cytochrome c reductase core protein II although reported earlier in testis are being reported for the first time in epididymal sperm. Furthermore, seven out of these eight novel proteins could be validated using peptide ELISA. These data are a useful repository, which could be exploited to develop targets for post-testicular immunocontraception or biomarkers for infertility diagnosis and management.<\/jats:p>","DOI":"10.1530\/rep-09-0052","type":"journal-article","created":{"date-parts":[[2009,5,7]],"date-time":"2009-05-07T20:54:27Z","timestamp":1241729667000},"page":"81-93","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":29,"title":["Identification of novel immunodominant epididymal sperm proteins using combinatorial approach"],"prefix":"10.1093","volume":"138","author":[{"given":"Shagufta A","family":"Khan","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amol R","family":"Suryawanshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandeep A","family":"Ranpura","sequence":"additional","affiliation":[{"name":"Department of Gamete Immunobiology National Institute for Research in Reproductive Health (ICMR), J M Street, Parel, Mumbai 400012, India 1Urological Diseases Research Centre Children's Hospital Boston, 300 Longwood Avenue, Boston, Massachusetts 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sudhir V","family":"Jadhav","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vrinda V","family":"Khole","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2009,7,1]]},"reference":[{"key":"2026010712511071700_bib1","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1002\/mrd.1080420311","article-title":"Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa","volume":"42","author":"Alvarez","year":"1995","journal-title":"Molecular Reproduction and Development"},{"key":"2026010712511071700_bib2","doi-asserted-by":"crossref","first-page":"1003","DOI":"10.1002\/pmic.200401100","article-title":"Identification of post-translational modifications that occur during sperm maturation using difference in two-dimensional gel electrophoresis","volume":"5","author":"Baker","year":"2005","journal-title":"Proteomics"},{"key":"2026010712511071700_bib3","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1002\/mrd.20643","article-title":"Molecular identities of human sperm proteins reactive with antibodies in sera of immunoinfertile women","volume":"74","author":"Bhande","year":"2007","journal-title":"Molecular Reproduction and Development"},{"key":"2026010712511071700_bib4","doi-asserted-by":"crossref","first-page":"915","DOI":"10.1093\/humrep\/deg207","article-title":"Immune infertility: towards a better understanding of sperm (auto)-immunity. The value of proteomic analysis","volume":"5","author":"Bohring","year":"2003","journal-title":"Human Reproduction"},{"key":"2026010712511071700_bib5","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1093\/molehr\/7.2.113","article-title":"Isolation and identification of sperm membrane antigens recognized by antisperm antibodies, and their possible role in immunological infertility disease","volume":"7","author":"Bohring","year":"2001","journal-title":"Molecular Human Reproduction"},{"key":"2026010712511071700_bib6","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1042\/bj1740741","article-title":"Activity and androgenic control of enzymes associated with the tricarboxylic acid cycle, lipid oxidation and mitochondrial shuttles in the epididymis and epididymal spermatozoa of the rat","volume":"174","author":"Brooks","year":"1978","journal-title":"Biochemical Journal"},{"key":"2026010712511071700_bib7","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1095\/biolreprod58.3.834","article-title":"Glyceraldehyde 3-phosphate dehydrogenase-S protein distribution during mouse spermatogenesis","volume":"58","author":"Bunch","year":"1998","journal-title":"Biology of Reproduction"},{"key":"2026010712511071700_bib8","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1074\/mcp.M500322-MCP200","article-title":"Proteomic profiling of accessory structures from the mouse sperm flagellum","volume":"5","author":"Cao","year":"2006","journal-title":"Molecular and Cellular Proteomics"},{"key":"2026010712511071700_bib9","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1006\/dbio.1994.1252","article-title":"The major fibrous sheath polypeptide of mouse sperm: structural and functional similarities to the A-kinase anchoring proteins","volume":"165","author":"Carrera","year":"1994","journal-title":"Developmental Biology"},{"key":"2026010712511071700_bib10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-3-642-71471-9","volume-title":"The Epididymis, Sperm Maturation and Fertilization","author":"Cooper","year":"1986"},{"key":"2026010712511071700_bib11","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1093\/humupd\/5.2.141","article-title":"Recent biochemical approaches to post testicular epididymal contraception","volume":"5","author":"Cooper","year":"1999","journal-title":"Human Reproduction Update"},{"key":"2026010712511071700_bib12","doi-asserted-by":"crossref","first-page":"648","DOI":"10.1016\/j.theriogenology.2006.09.041","article-title":"Heparin and quercitin generate differential metabolic pathways that involve aminotransferases and LDH-X dehydrogenase in cryopreserved bovine spermatozoa","volume":"67","author":"C\u00f3rdoba","year":"2007","journal-title":"Theriogenology"},{"key":"2026010712511071700_bib13","first-page":"1653","article-title":"The CRES gene: a unique testis regulated gene related to the Cystatin family is highly restricted to the proximal region of the mouse epididymis","volume":"6","author":"Cornwall","year":"1992","journal-title":"Molecular Endocrinology"},{"key":"2026010712511071700_bib14","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/S1043-2760(99)00220-9","article-title":"Male contraception: views to the 21st century, Bethesda, MD, USA, 9\u201310 September 1999","volume":"11","author":"DePaolo","year":"2000","journal-title":"Trends in Endocrinology and Metabolism"},{"key":"2026010712511071700_bib15","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1093\/molehr\/gam026","article-title":"Application of proteomic methods for identification of sperm immunogenic antigens","volume":"13","author":"Domaga\u0142a","year":"2007","journal-title":"Molecular Human Reproduction"},{"key":"2026010712511071700_bib16","doi-asserted-by":"crossref","first-page":"9703","DOI":"10.1016\/S0021-9258(18)92877-2","article-title":"Characterization of a GTPase-activating protein for the Ras-related Ral protein","volume":"266","author":"Emkey","year":"1991","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712511071700_bib17","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1002\/j.1939-4640.1991.tb01606.x","article-title":"Use of neonatal tolerization and chemical immunosuppression for the production of monoclonal antibodies to maturation specific sperm surface molecules","volume":"12","author":"Ensrud","year":"1991","journal-title":"Journal of Andrology"},{"key":"2026010712511071700_bib18","doi-asserted-by":"crossref","first-page":"11579","DOI":"10.1074\/jbc.M202325200","article-title":"Phosphoproteome analysis of capacitated human sperm. Evidence of tyrosine phosphorylation of a kinase-anchoring protein 3 and valosin-containing protein\/p97 during capacitation","volume":"278","author":"Ficarro","year":"2003","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712511071700_bib19","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1095\/biolreprod25.4.871","article-title":"Regional differences in synthesis, intracellular transport and secretion of protein in mouse epididymis","volume":"2","author":"Flickinger","year":"1981","journal-title":"Biology of Reproduction"},{"key":"2026010712511071700_bib20","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1002\/j.1939-4640.2004.tb02861.x","article-title":"Electrophoretic characterization of the human sperm-specific enolase at different stages of maturation","volume":"25","author":"Force","year":"2004","journal-title":"Journal of Andrology"},{"key":"2026010712511071700_bib21","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1078\/S0171-9335(04)70012-6","article-title":"The glycolytic enzyme enolase is present in sperm tail and displays nucleotide-dependent association with microtubules","volume":"79","author":"Gitlits","year":"2000","journal-title":"European Journal of Cell Biology"},{"key":"2026010712511071700_bib22","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1530\/rep.1.00694","article-title":"Expression of Col1a1, Col1a2 and procollagen I in germ cells of immature and adult mouse testis","volume":"130","author":"He","year":"2005","journal-title":"Reproduction"},{"key":"2026010712511071700_bib23","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1093\/oxfordjournals.humrep.a137318","article-title":"Immunochemical localization and characterization of proteins from mouse cauda epididymis using human sperm specific monoclonal antibody","volume":"6","author":"Hegde","year":"1991","journal-title":"Human Reproduction"},{"key":"2026010712511071700_bib24","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1002\/j.1939-4640.1998.tb02054.x","article-title":"Androgen binding protein secretion and endocytosis by principal cells in the adult rat epididymis and during postnatal development","volume":"19","author":"Hermo","year":"1998","journal-title":"Journal of Andrology"},{"key":"2026010712511071700_bib25","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1095\/biolreprod61.2.428","article-title":"Sperm mitochondria-associated cysteine-rich protein (SMCP) is an autoantigen in Lewis rats","volume":"61","author":"Herr","year":"1999","journal-title":"Biology of Reproduction"},{"key":"2026010712511071700_bib26","doi-asserted-by":"crossref","first-page":"15281","DOI":"10.1074\/jbc.M313433200","article-title":"Voltage-dependent anion-selective channels VDAC2 and VDAC3 are abundant proteins in bovine outer dense fibers, a cytoskeletal component of the sperm flagellum","volume":"279","author":"Hinsch","year":"2004","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712511071700_bib27","doi-asserted-by":"crossref","first-page":"723","DOI":"10.1016\/j.bbapap.2007.03.017","article-title":"Disulphide bond reduction and S-carboxamidomethylation of PSP94 affects its conformation but not the ability to bind immunoglobulin","volume":"1774","author":"Jagtap","year":"2007","journal-title":"Biochimica et Biophysica Acta"},{"key":"2026010712511071700_bib28","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1093\/humupd\/2.2.87","article-title":"Role of free l-carnitine and acetyl-l-carnitine in post-gonadal maturation of mammalian spermatozoa","volume":"2","author":"Jeulin","year":"1996","journal-title":"Human Reproduction Update"},{"key":"2026010712511071700_bib29","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1095\/biolreprod.105.039719","article-title":"The mouse epididymal transcriptome: transcriptional profiling of segmental gene expression in the epididymis","volume":"73","author":"Johnston","year":"2005","journal-title":"Biology of Reproduction"},{"key":"2026010712511071700_bib30","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1093\/oxfordjournals.humrep.a138603","article-title":"Gamete immunology","volume":"9","author":"Jones","year":"1994","journal-title":"Human Reproduction"},{"key":"2026010712511071700_bib31","first-page":"73","article-title":"Plasma membrane structure and remodelling during sperm maturation in the epididymis","volume":"53","author":"Jones","year":"1998","journal-title":"Journal of Reproduction and Fertility Supplement"},{"key":"2026010712511071700_bib32","doi-asserted-by":"crossref","first-page":"524","DOI":"10.1002\/j.1939-4640.2003.tb02704.x","article-title":"Monoclonal antibodies to epididymis specific proteins using mice rendered to testicular proteins","volume":"24","author":"Joshi","year":"2003","journal-title":"Journal of Andrology"},{"key":"2026010712511071700_bib33","first-page":"764","article-title":"Epididymis as a target for contraception","volume":"41","author":"Khole","year":"2003","journal-title":"Indian Journal of Experimental Biology"},{"key":"2026010712511071700_bib34","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1111\/j.8755-8920.2000.440606.x","article-title":"Identification of epididymis specific antigen by neonatal tolerization","volume":"44","author":"Khole","year":"2000","journal-title":"American Journal of Reproductive Immunology"},{"key":"2026010712511071700_bib35","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/j.ydbio.2006.10.004","article-title":"Compartmentalization of a unique ADP\/ATP carrier protein SFEC (Sperm Flagellar Energy Carrier, AAC4) with glycolytic enzymes in the fibrous sheath of the human sperm flagellar principal piece","volume":"302","author":"Kim","year":"2007","journal-title":"Developmental Biology"},{"key":"2026010712511071700_bib36","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1530\/ror.0.0030086","article-title":"Molecular characterization of epididymal proteins","volume":"3","author":"Kirchhoff","year":"1998","journal-title":"Reviews of Reproduction"},{"key":"2026010712511071700_bib37","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/S0074-7696(08)61567-3","article-title":"Gene expression in the epididymis","volume":"188","author":"Kirchhoff","year":"1999","journal-title":"International Review of Cytology"},{"key":"2026010712511071700_bib38","doi-asserted-by":"crossref","first-page":"1017","DOI":"10.1095\/biolreprod33.4.1017","article-title":"Synthesis and secretion of proteins by perfused caput epididymal tubule and association of secreted proteins with spermatozoa","volume":"33","author":"Klinefelter","year":"1985","journal-title":"Biology of Reproduction"},{"key":"2026010712511071700_bib39","doi-asserted-by":"crossref","first-page":"1601","DOI":"10.1095\/biolreprod63.6.1601","article-title":"Isolation and characterization of a haploid germ cell-specific novel complementary deoxyribonucleic acid; testis-specific homologue of succinyl CoA:3-Oxo acid CoA transferase","volume":"63","author":"Koga","year":"2000","journal-title":"Biology of Reproduction"},{"key":"2026010712511071700_bib40","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1016\/0014-5793(90)80535-Q","article-title":"Semi-empirical method for prediction of antigenic determinants on protein antigens","volume":"276","author":"Kolaskar","year":"1990","journal-title":"FEBS Letters"},{"key":"2026010712511071700_bib41","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.jri.2007.06.001","article-title":"Immunogenicity of a multi-component recombinant human acrosomal protein vaccine in female Macaca fascicularis","volume":"77","author":"Kurth","year":"2008","journal-title":"Journal of Reproductive Immunology"},{"key":"2026010712511071700_bib42","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/S1472-6483(10)61885-8","article-title":"Physiological and proteomic approaches to studying prefertilization events in the human","volume":"7","author":"Lefievre","year":"2003","journal-title":"Reproductive Biomedicine Online"},{"key":"2026010712511071700_bib43","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1111\/j.1365-2605.1978.tb00511.x","article-title":"Purification and localization of acidic epididymal glycoprotein (AEG): a sperm coating antigen secreted by the rat epididymis","volume":"2","author":"Leo","year":"1978","journal-title":"International Journal of Andrology"},{"key":"2026010712511071700_bib44","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712511071700_bib45","doi-asserted-by":"crossref","first-page":"2197","DOI":"10.1016\/S0021-9258(18)97446-6","article-title":"Effects of testosterone on the distribution of carnitine, acetylcarnitine, and carnitine acetyltransferase in tissues of the reproductive system","volume":"240","author":"Marquis","year":"1965","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712511071700_bib46","doi-asserted-by":"crossref","first-page":"4356","DOI":"10.1002\/pmic.200600094","article-title":"Proteomic identification of human sperm proteins","volume":"6","author":"Mart\u00ednez-Heredia","year":"2006","journal-title":"Proteomics"},{"key":"2026010712511071700_bib47","doi-asserted-by":"crossref","first-page":"16501","DOI":"10.1073\/pnas.0407708101","article-title":"Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility","volume":"101","author":"Miki","year":"2004","journal-title":"PNAS"},{"key":"2026010712511071700_bib48","doi-asserted-by":"crossref","first-page":"25743","DOI":"10.1074\/jbc.M500310200","article-title":"Novelty of the pyruvate metabolic enzyme dihydrolipoamide dehydrogenase in spermatozoa","volume":"280","author":"Mitra","year":"2005","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712511071700_bib49","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1002\/(SICI)1098-2795(199804)49:4<374::AID-MRD4>3.0.CO;2-K","article-title":"Mouse spermatogenic cell-specific type 1 hexokinase (mHk1-s) transcripts are expressed by alternative splicing from the mHk1 gene and the HK1-S protein is localized mainly in the sperm tail","volume":"49","author":"Mori","year":"1998","journal-title":"Molecular Reproduction and Development"},{"key":"2026010712511071700_bib50","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1095\/biolreprod56.3.771","article-title":"Two-dimensional gel electrophoretic analysis of vectorially labelled surface proteins of human spermatozoa","volume":"56","author":"Naaby-Hansen","year":"1997","journal-title":"Biology of Reproduction"},{"key":"2026010712511071700_bib51","doi-asserted-by":"crossref","first-page":"236","DOI":"10.1006\/dbio.2001.0527","article-title":"CABYR, a novel calcium-binding tyrosine phosphorylation-regulated fibrous sheath protein involved in capacitation","volume":"242","author":"Naaby-Hansen","year":"2002","journal-title":"Developmental Biology"},{"key":"2026010712511071700_bib52","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1095\/biolreprod.104.033530","article-title":"Tyrosine phosphorylation generates multiple isoforms of the mitochondrial capsule protein, phospholipid hydroperoxide glutathione peroxidase (PHGPx), during hamster sperm capacitation","volume":"72","author":"Nagdas","year":"2005","journal-title":"Biology of Reproduction"},{"key":"2026010712511071700_bib53","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1002\/j.1939-4640.1996.tb01763.x","article-title":"Hexokinase present in human sperm is not tyrosine phosphorylated but its antibodies affect fertilizing capacity","volume":"17","author":"Naz","year":"1996","journal-title":"Journal of Andrology"},{"key":"2026010712511071700_bib54","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1095\/biolreprod39.1.169","article-title":"Comparative analysis of proteins from the fibrous sheath and outer dense fibers of rat spermatozoa","volume":"39","author":"Oko","year":"1988","journal-title":"Biology of Reproduction"},{"key":"2026010712511071700_bib55","doi-asserted-by":"crossref","first-page":"2138","DOI":"10.1210\/me.2003-0008","article-title":"Discover insilico and characterization in vitro of novel genes exclusively expressed in the mouse epididymis","volume":"17","author":"Penttinen","year":"2003","journal-title":"Molecular Endocrinology"},{"key":"2026010712511071700_bib56","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1095\/biolreprod42.6.929","article-title":"Identification of human sperm surface glycoproteins recognized by autoantisera from immune infertile men, women, and vasectomized men","volume":"42","author":"Primakoff","year":"1990","journal-title":"Biology of Reproduction"},{"key":"2026010712511071700_bib57","doi-asserted-by":"crossref","first-page":"1183","DOI":"10.1095\/biolreprod.108.068734","article-title":"Proteomic analysis of bovine sperm YWHA binding partners identify proteins involved in signaling and metabolism","volume":"79","author":"Puri","year":"2008","journal-title":"Biology of Reproduction"},{"key":"2026010712511071700_bib58","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0378-1119(01)00462-0","article-title":"Cloning and sequencing of human Eppin: a novel family of protease inhibitors expressed in the epididymis and testis","volume":"270","author":"Richardson","year":"2001","journal-title":"Gene"},{"key":"2026010712511071700_bib59","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1095\/biolreprod61.1.61","article-title":"Human sperm proteome: immunodominant sperm surface antigens identified with sera from infertile men and women","volume":"61","author":"Shetty","year":"1999","journal-title":"Biology of Reproduction"},{"key":"2026010712511071700_bib60","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.jri.2007.04.006","article-title":"Human sperm protein encyclopedia and alloantigen index: mining novel allo-antigens using sera from ASA-positive infertile patients and vasectomized men","volume":"77","author":"Shetty","year":"2008","journal-title":"Journal of Reproductive Immunology"},{"key":"2026010712511071700_bib61","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1021\/ac950914h","article-title":"Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels","volume":"68","author":"Shevchenko","year":"1996","journal-title":"Analytical Chemistry"},{"key":"2026010712511071700_bib62","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1530\/reprod\/119.2.241","article-title":"Maturation-dependent glycoproteins containing both N- and a-linked oligosaccharides in epididymal sperm plasma membrane of rhesus monkeys (Macaca mulatta)","volume":"119","author":"Srivastav","year":"2000","journal-title":"Journal of Reproduction and Fertility"},{"key":"2026010712511071700_bib63","doi-asserted-by":"crossref","first-page":"3533","DOI":"10.1002\/pmic.200500845","article-title":"Proteomic analysis of sperm regions that mediate sperm\u2013egg interactions","volume":"6","author":"Stein","year":"2006","journal-title":"Proteomics"},{"key":"2026010712511071700_bib64","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1186\/1477-7827-2-10","article-title":"Sperm specific proteins \u2013 potential candidate molecules for fertility control","volume":"2","author":"Suri","year":"2004","journal-title":"Reproductive Biology and Endocrinology"},{"key":"2026010712511071700_bib65","doi-asserted-by":"crossref","first-page":"1665","DOI":"10.1242\/jcs.114.9.1665","article-title":"A putative, ubiquitin dependent mechanism for the recognition and elimination of defective spermatozoa","volume":"114","author":"Sutovsky","year":"2001","journal-title":"Journal of Cell Science"},{"key":"2026010712511071700_bib66","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1095\/biolreprod55.5.956","article-title":"Characterization and identification of proteins secreted in the various regions of the adult boar epididymis","volume":"55","author":"Syntin","year":"1996","journal-title":"Biology of Reproduction"},{"key":"2026010712511071700_bib67","doi-asserted-by":"crossref","first-page":"2121","DOI":"10.1101\/gr.2596504","article-title":"The status, quality, and expansion of the NIH full-length cDNA project: the mammalian gene collection (MGC)","volume":"14","author":"The MGC Project Team","year":"2004","journal-title":"Genome Research"},{"key":"2026010712511071700_bib68","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1095\/biolreprod.102.010975","article-title":"Ubiquitination of prohibitin in mammalian sperm mitochondria: possible roles in the regulation of mitochondrial inheritance and sperm quality control","volume":"69","author":"Thompson","year":"2003","journal-title":"Biology of Reproduction"},{"key":"2026010712511071700_bib69","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1073\/pnas.76.9.4350","article-title":"Electrophoretic transfer of proteins from polyacrylamide gel to nitrocellulose sheet: procedures and applications","volume":"76","author":"Towbin","year":"1979","journal-title":"PNAS"},{"key":"2026010712511071700_bib70","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1091\/mbc.9.2.263","article-title":"Targeting of a germ cell-specific type 1 hexokinase lacking a porin-binding domain to the mitochondria as well as to the head and fibrous sheath of murine spermatozoa","volume":"9","author":"Travis","year":"1998","journal-title":"Molecular Biology of the Cell"},{"key":"2026010712511071700_bib71","doi-asserted-by":"crossref","first-page":"302","DOI":"10.1002\/j.1939-4640.2001.tb02184.x","article-title":"Molecular genetic analysis of two human sperm fibrous sheath proteins, AKAP4 and AKAP3, in men with dysplasia of the fibrous sheath","volume":"22","author":"Turner","year":"2001","journal-title":"Journal of Andrology"},{"key":"2026010712511071700_bib72","doi-asserted-by":"crossref","first-page":"761","DOI":"10.2164\/jandrol.05045","article-title":"Monoclonal antibody from vasectomized mouse identifies a conserved testis-specific antigen TSA70","volume":"26","author":"Wakle","year":"2005","journal-title":"Journal of Andrology"},{"key":"2026010712511071700_bib73","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1007\/978-0-387-75841-1_5","volume-title":"Immunogenicity of Biopharmaceuticals","author":"Weert","year":"2008"},{"key":"2026010712511071700_bib74","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1002\/j.1939-4640.2000.tb02111.x","article-title":"Human glyceraldehyde 3-phosphate dehydrogenase-2 gene is expressed specifically in spermatogenic cells","volume":"21","author":"Welch","year":"2000","journal-title":"Journal of Andrology"},{"key":"2026010712511071700_bib75","doi-asserted-by":"crossref","first-page":"1821","DOI":"10.1242\/jcs.110.15.1821","article-title":"Glyceraldehyde 3-phosphate dehydrogenase is bound to the fibrous sheath of mammalian spermatozoa","volume":"110","author":"Westhoff","year":"1997","journal-title":"Journal of Cell Science"},{"key":"2026010712511071700_bib76","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1095\/biolreprod66.1.241","article-title":"Tektin B1 demonstrates flagellar localization in human sperm","volume":"66","author":"Wolkowicz","year":"2002","journal-title":"Biology of Reproduction"}],"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rep.bioscientifica.com\/view\/journals\/rep\/138\/1\/81.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/138\/1\/81\/64531882\/81.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/138\/1\/81\/64531882\/81.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T17:54:56Z","timestamp":1767808496000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/138\/1\/81\/8243124"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,7,1]]},"references-count":76,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,7,1]]}},"URL":"https:\/\/doi.org\/10.1530\/rep-09-0052","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2009,7,1]]},"published":{"date-parts":[[2009,7,1]]}}}