{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:40:03Z","timestamp":1791168003038,"version":"4.1.0"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2009,7,1]],"date-time":"2009-07-01T00:00:00Z","timestamp":1246406400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The fertility of ram semen after cervical insemination is substantially reduced by 24\u200ah of storage in liquid form. The effects of liquid storage on the transit of ram spermatozoa in the ewe genital tract was investigated using a new procedure allowing direct observation of the spermatozoa in the genital tract. Ejaculated ram spermatozoa were double labeled with R18 and MitoTracker Green FM, and used to inseminate ewes in estrus either cervically through the vagina or laparoscopically into the base of the uterine horns. Four hours after insemination, the spermatozoa were directly observed in situ using fibered confocal fluorescence microscopy in the base, middle and tip of the uterine horns, the utero-tubal junction (UTJ) and the oviduct. The high resolution video images obtained with this technique allowed determination of the distribution of spermatozoa and individual motility in the lumen of the ewe's genital tract. The results showed a gradient of increasing concentration of spermatozoa from the base of the uterus to the UTJ 4\u200ah after intra-uterine insemination into the base of the horns. The UTJ was shown to be a storage region for spermatozoa before their transfer to the oviduct. The in vitro storage of spermatozoa in liquid form decreased their migration through the cervix and reduced the proportion of motile spermatozoa and their straight line velocity at the UTJ and their transit into the oviduct.<\/jats:p>","DOI":"10.1530\/rep-09-0108","type":"journal-article","created":{"date-parts":[[2009,5,7]],"date-time":"2009-05-07T20:54:27Z","timestamp":1241729667000},"page":"45-53","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":60,"title":["<i>In vivo<\/i>\n                    imaging of\n                    <i>in situ<\/i>\n                    motility of fresh and liquid stored ram spermatozoa in the ewe genital tract"],"prefix":"10.1093","volume":"138","author":[{"given":"Xavier","family":"Druart","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliette","family":"Cogni\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e9rard","family":"Baril","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9rique","family":"Cl\u00e9ment","sequence":"additional","affiliation":[{"name":"UMR 6175 INRA CNRS-Universit\u00e9 de Tours-Haras Nationaux, Station de Physiologie de la Reproduction et des Comportements, Institut National de la Recherche Agronomique, 37380 Nouzilly, France 1INRIA Paris-Rocquencourt Domaine de Voluceau, Rocquencourt BP 105, 78153 Le Chesnay Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Louis","family":"Dacheux","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Luc","family":"Gatti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,7,1]]},"reference":[{"key":"2026010712500722100_bib1","first-page":"63","article-title":"In vitro and in vivo motility studies of 99mTc HM-PAO labelled sperm cells","volume":"43","author":"Balogh","year":"1995","journal-title":"Acta Veterinaria Hungarica"},{"key":"2026010712500722100_bib2","first-page":"1134","article-title":"Sperm cell dynamics in the female rabbit genital tract after insemination monitored by radiolabeled spermatozoa","volume":"34","author":"Bockisch","year":"1993","journal-title":"Journal of Nuclear Medicine"},{"key":"2026010712500722100_bib3","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1562\/0031-8655(2000)071<0730:APISTC>2.0.CO;2","article-title":"Autofluorescence patterns in short-term cultures of normal cervical tissue","volume":"71","author":"Brookner","year":"2000","journal-title":"Photochemistry and Photobiology"},{"key":"2026010712500722100_bib4","first-page":"297","article-title":"Use of 131I labeling for studying the distribution and migration of spermatozoa in the genital tract of female sheep following artificial insemination. 1. 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