{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T21:29:34Z","timestamp":1781126974185,"version":"3.54.1"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2010,1,1]],"date-time":"2010-01-01T00:00:00Z","timestamp":1262304000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The adaptive growth of the uterus is a critical event that involves changes in cellular phenotypes throughout pregnancy. In early pregnancy, uterine growth is due to hyperplasia of uterine smooth muscle cells (SMCs) within the myometrium; however, the major component of myometrial growth occurs after mid-gestation. This study sought to test the hypothesis that increase in myometrial growth seen during late pregnancy is due to SMC hypertrophy caused by mechanical stretch of uterine tissue by a growing fetus(es) by providing direct measurements of individual SMC size. We employed a stereological approach to calculate the average cell volumes of uterine myocytes through diameter measurements using the Stereoinvestigator statistical software. Uterine tissues were collected from nonpregnant Wistar rats, as well as from gravid and nongravid horns of unilaterally pregnant animals on gestational days (d) 8 (early gestation), 14 (mid-gestation), 19 (late gestation), 22 (term), and 4 days post partum. Anti-caveolin-1 immunostaining was used to clearly delineate SMC boundaries. The stereological analysis revealed that the dramatic increase in myometrial growth seen during late gestation (d19\u201322) is due to a threefold increase in the size of uterine myocytes. A significant increase in SMC volumes was detected in the gravid uterine horn as compared with the corresponding empty horn of unilateral term pregnant animals (day 22, mean cell volume 1114 vs 361\u200a\u03bcm3, P&amp;lt;0.05), indicating the effect of uterine occupancy. The restriction of the hypertrophy to cells within the gravid horn suggests that it may be a response to the biological mechanical stretch of uterine walls by the growing fetus(es) and placenta(s).<\/jats:p>","DOI":"10.1530\/rep-09-0260","type":"journal-article","created":{"date-parts":[[2009,9,23]],"date-time":"2009-09-23T20:54:24Z","timestamp":1253739264000},"page":"247-253","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":69,"title":["Mechanical stretch regulates hypertrophic phenotype of the myometrium during pregnancy"],"prefix":"10.1093","volume":"139","author":[{"given":"Oksana","family":"Shynlova","sequence":"first","affiliation":[{"name":"Samuel Lunenfeld Research Institute Mount Sinai Hospital, 25 Orde Street, Room 6-1019, Toronto, Ontario, Canada M5T 3H7 and Departments of 2Physiology 3Obstetrics and Gynecology University of Toronto, Toronto, Ontario, Canada M5S 1A1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Kwong","sequence":"additional","affiliation":[{"name":"Samuel Lunenfeld Research Institute Mount Sinai Hospital, 25 Orde Street, Room 6-1019, Toronto, Ontario, Canada M5T 3H7 and Departments of 2Physiology 3Obstetrics and Gynecology University of Toronto, Toronto, Ontario, Canada M5S 1A1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen J","family":"Lye","sequence":"additional","affiliation":[{"name":"Samuel Lunenfeld Research Institute Mount Sinai Hospital, 25 Orde Street, Room 6-1019, Toronto, Ontario, Canada M5T 3H7 and Departments of 2Physiology 3Obstetrics and Gynecology University of Toronto, Toronto, Ontario, Canada M5S 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