{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T23:51:31Z","timestamp":1767829891335,"version":"3.49.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T00:00:00Z","timestamp":1275350400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Basigin plays important roles in both male and female reproduction because basigin (Bsg) null male and female mice are infertile. The aim of the present study was to determine whether basigin expression in reproductive organs requires estrogen receptor-\u03b1 (ESR1, ER\u03b1) or -\u03b2 (ESR2, ER\u03b2). Expression of basigin protein in the testis, ovary, and male and female reproductive tracts was studied in adult wild-type (WT), Esr1-null (\u03b1ERKO), and Esr2-null (\u03b2ERKO) mice by immunohistochemistry and immunoblotting. Basigin mRNA levels in ovary and uterus were examined by quantitative RT-PCR. In females, basigin protein expression was observed mainly in granulosa and interstitial cells of the ovary and epithelial cells of the proximal oviduct in all genotypes. Basigin protein was also expressed in the uterine epithelium at proestrus and estrus in WT and \u03b2ERKO mice but not in \u03b1ERKO mice. However, a higher level of basigin mRNA was observed in uteri of \u03b1ERKO mice compared with WT and \u03b2ERKO mice. In males, basigin was expressed in Leydig cells and all germ cells except spermatogonia in all genotypes. Basigin was present in epithelial cells lining the efferent ductules in WT and \u03b2ERKO mice, but expression was greatly reduced in \u03b1ERKO mice. In epididymal ducts, basigin expression was observed in epithelial cells in the caput and cauda in all genotypes. These data suggest that expression of basigin protein requires ESR1, but not ESR2, in the uterus and efferent ductules, but is independent of estrogen receptor in the ovary, oviduct, testis, and epididymis.<\/jats:p>","DOI":"10.1530\/rep-10-0069","type":"journal-article","created":{"date-parts":[[2010,4,13]],"date-time":"2010-04-13T21:53:21Z","timestamp":1271195601000},"page":"1057-1066","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":15,"title":["Expression of basigin in reproductive tissues of estrogen receptor-\u03b1 or -\u03b2 null mice"],"prefix":"10.1093","volume":"139","author":[{"given":"Li","family":"Chen","sequence":"first","affiliation":[{"name":"Departments of 1Animal Sciences 2Veterinary Biosciences University of Illinois, Urbana, Illinois 61801, USA 3Laboratory of Reproductive and Developmental Toxicology National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiajia","family":"Bi","sequence":"additional","affiliation":[{"name":"Departments of 1Animal Sciences 2Veterinary Biosciences University of Illinois, Urbana, Illinois 61801, USA 3Laboratory of Reproductive and Developmental Toxicology National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masaaki","family":"Nakai","sequence":"additional","affiliation":[{"name":"Departments of 1Animal Sciences 2Veterinary Biosciences University of Illinois, Urbana, Illinois 61801, USA 3Laboratory of Reproductive and Developmental Toxicology National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Bunick","sequence":"additional","affiliation":[{"name":"Departments of 1Animal Sciences 2Veterinary Biosciences University of Illinois, Urbana, Illinois 61801, USA 3Laboratory of Reproductive and Developmental Toxicology National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John F","family":"Couse","sequence":"additional","affiliation":[{"name":"Departments of 1Animal Sciences 2Veterinary Biosciences University of Illinois, Urbana, Illinois 61801, USA 3Laboratory of Reproductive and Developmental Toxicology National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth S","family":"Korach","sequence":"additional","affiliation":[{"name":"Departments of 1Animal Sciences 2Veterinary Biosciences University of Illinois, Urbana, Illinois 61801, USA 3Laboratory of Reproductive and Developmental Toxicology National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romana A","family":"Nowak","sequence":"additional","affiliation":[{"name":"Departments of 1Animal Sciences 2Veterinary Biosciences University of Illinois, Urbana, Illinois 61801, USA 3Laboratory of Reproductive and Developmental Toxicology National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina 27709, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2010,6,1]]},"reference":[{"key":"2026010712522446800_bib1","doi-asserted-by":"crossref","first-page":"350","DOI":"10.1038\/nature02871","article-title":"The functions of animal microRNAs","volume":"431","author":"Ambros","year":"2004","journal-title":"Nature"},{"key":"2026010712522446800_bib2","doi-asserted-by":"crossref","first-page":"517","DOI":"10.4049\/jimmunol.175.1.517","article-title":"Extracellular cyclophilins contribute to the regulation of inflammatory responses","volume":"175","author":"Arora","year":"2005","journal-title":"Journal of Immunology"},{"key":"2026010712522446800_bib3","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/0304-3835(84)90137-X","article-title":"Collagenase stimulation in cocultures of human fibroblasts and human tumor cells","volume":"24","author":"Biswas","year":"1984","journal-title":"Cancer Letters"},{"key":"2026010712522446800_bib4","first-page":"434","article-title":"The human tumor cell-derived collagenase stimulatory factor (renamed EMMPRIN) is a member of the immunoglobulin superfamily","volume":"55","author":"Biswas","year":"1995","journal-title":"Cancer Research"},{"key":"2026010712522446800_bib5","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1002\/mrd.20060","article-title":"Basigin expression and regulation in mouse ovary during the sexual maturation and development of corpus luteum","volume":"68","author":"Chang","year":"2004","journal-title":"Molecular Reproduction and Development"},{"key":"2026010712522446800_bib6","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1007\/s004180100282","article-title":"Ultrastructural localization of basigin in normal human epidermis","volume":"115","author":"Chen","year":"2001","journal-title":"Histochemistry and Cell Biology"},{"key":"2026010712522446800_bib7","doi-asserted-by":"crossref","first-page":"2874","DOI":"10.1210\/endo-128-6-2874","article-title":"Estrogen receptor expression in developing epididymis, efferent ductules, and other male reproductive organs","volume":"128","author":"Cooke","year":"1991","journal-title":"Endocrinology"},{"key":"2026010712522446800_bib8","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1210\/edrv.20.3.0370","article-title":"Estrogen receptor null mice: what have we learned and where will they lead us?","volume":"20","author":"Couse","year":"1999","journal-title":"Endocrine Reviews"},{"key":"2026010712522446800_bib9","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1023\/A:1022696012668","article-title":"EMMPRIN (extracellular matrix metalloproteinase inducer) is a novel marker of poor outcome in serous ovarian carcinoma","volume":"20","author":"Davidson","year":"2003","journal-title":"Clinical & Experimental Metastasis"},{"key":"2026010712522446800_bib10","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/S0168-0102(97)00119-3","article-title":"Expression of basigin, a member of the immunoglobulin superfamily, in the mouse central nervous system","volume":"30","author":"Fan","year":"1998","journal-title":"Neuroscience Research"},{"key":"2026010712522446800_bib11","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1074\/jbc.272.1.24","article-title":"Stimulation of matrix metalloproteinase production by recombinant extracellular matrix metalloproteinase inducer from transfected Chinese hamster ovary cells","volume":"272","author":"Guo","year":"1997","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712522446800_bib12","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1038\/37352","article-title":"A role for oestrogens in the male reproductive system","volume":"390","author":"Hess","year":"1997","journal-title":"Nature"},{"key":"2026010712522446800_bib13","first-page":"3128","article-title":"Retinal dysfunction in basigin deficiency","volume":"41","author":"Hori","year":"2000","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"2026010712522446800_bib14","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1006\/bbrc.1996.0980","article-title":"Roles of basigin, a member of the immunoglobulin superfamily, in behavior as to an irritating odor, lymphocyte response, and blood\u2013brain barrier","volume":"224","author":"Igakura","year":"1996","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"2026010712522446800_bib15","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1006\/dbio.1997.8819","article-title":"A null mutation in basigin, an immunoglobulin superfamily member, indicates its important roles in peri-implantation development and spermatogenesis","volume":"194","author":"Igakura","year":"1998","journal-title":"Developmental Biology"},{"key":"2026010712522446800_bib16","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1097\/01.pgp.0000189244.57145.84","article-title":"Increasing expression of extracellular matrix metalloprotease inducer in ovary tumors: tissue microarray analysis of immunostaining score with clinicopathological parameters","volume":"25","author":"Jin","year":"2006","journal-title":"International Journal of Gynecological Pathology"},{"key":"2026010712522446800_bib17","first-page":"3154","article-title":"Tumor cell-derived collagenase-stimulatory factor increases expression of interstitial collagenase, stromelysin, and 72-kDa gelatinase","volume":"53","author":"Kataoka","year":"1993","journal-title":"Cancer Research"},{"key":"2026010712522446800_bib18","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1002\/1529-0131(200002)43:2<275::AID-ANR6>3.0.CO;2-#","article-title":"Increased expression of extracellular matrix metalloproteinase inducer in rheumatoid synovium","volume":"43","author":"Konttinen","year":"2000","journal-title":"Arthritis and Rheumatism"},{"key":"2026010712522446800_bib19","doi-asserted-by":"crossref","first-page":"15677","DOI":"10.1073\/pnas.95.26.15677","article-title":"Generation and reproductive phenotypes of mice lacking estrogen receptor beta","volume":"95","author":"Krege","year":"1998","journal-title":"PNAS"},{"key":"2026010712522446800_bib20","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0014-5793(98)00213-0","article-title":"Female sterility in mice lacking the basigin gene, which encodes a transmembrane glycoprotein belonging to the immunoglobulin superfamily","volume":"425","author":"Kuno","year":"1998","journal-title":"FEBS Letters"},{"key":"2026010712522446800_bib21","doi-asserted-by":"crossref","first-page":"1534","DOI":"10.1095\/biolreprod65.5.1534","article-title":"Estrogen regulation of ion transporter messenger RNA levels in mouse efferent ductules are mediated differentially through estrogen receptor (ER) alpha and ER beta","volume":"65","author":"Lee","year":"2001","journal-title":"Biology of Reproduction"},{"key":"2026010712522446800_bib22","doi-asserted-by":"crossref","first-page":"11162","DOI":"10.1073\/pnas.90.23.11162","article-title":"Alteration of reproductive function but not prenatal sexual development after insertional disruption of the mouse estrogen receptor gene","volume":"90","author":"Lubahn","year":"1993","journal-title":"PNAS"},{"key":"2026010712522446800_bib23","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1679\/aohc.61.405","article-title":"Stage-specific localization of basigin, a member of the immunoglobulin superfamily, during mouse spermatogenesis","volume":"61","author":"Maekawa","year":"1998","journal-title":"Archives of Histology and Cytology"},{"key":"2026010712522446800_bib24","doi-asserted-by":"crossref","first-page":"1229","DOI":"10.1158\/0008-5472.CAN-03-2832","article-title":"Emmprin promotes anchorage-independent growth in human mammary carcinoma cells by stimulating hyaluronan production","volume":"64","author":"Marieb","year":"2004","journal-title":"Cancer Research"},{"key":"2026010712522446800_bib25","first-page":"7575","article-title":"Regulation of extracellular matrix metalloproteinase inducer and matrix metalloproteinase expression by amphiregulin in transformed human breast epithelial cells","volume":"63","author":"Menashi","year":"2003","journal-title":"Cancer Research"},{"key":"2026010712522446800_bib26","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1593\/neo.07133","article-title":"Tumor vesicle-associated CD147 modulates the angiogenic capability of endothelial cells","volume":"9","author":"Millimaggi","year":"2007","journal-title":"Neoplasia"},{"key":"2026010712522446800_bib27","first-page":"981","article-title":"Basigin (CD147): a multifunctional transmembrane protein involved in reproduction, neural function, inflammation and tumor invasion","volume":"18","author":"Muramatsu","year":"2003","journal-title":"Histology and Histopathology"},{"key":"2026010712522446800_bib28","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1002\/ar.a.20320","article-title":"Tissue distribution of basigin and monocarboxylate transporter 1 in the adult male mouse: a study using the wild-type and basigin gene knockout mice","volume":"288","author":"Nakai","year":"2006","journal-title":"Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology"},{"key":"2026010712522446800_bib29","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1006\/bbrc.1997.6993","article-title":"Abnormalities of sensory and memory functions in mice lacking Bsg gene","volume":"236","author":"Naruhashi","year":"1997","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"2026010712522446800_bib30","doi-asserted-by":"crossref","first-page":"6063","DOI":"10.1210\/jc.2003-030457","article-title":"Identification and characterization of extracellular matrix metalloproteinase inducer in human endometrium during the menstrual cycle in vivo and in vitro","volume":"88","author":"Noguchi","year":"2003","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"key":"2026010712522446800_bib31","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1006\/exer.2000.0974","article-title":"Retinal degeneration following failed photoreceptor maturation in 5A11\/basigin null mice","volume":"72","author":"Ochrietor","year":"2001","journal-title":"Experimental Eye Research"},{"key":"2026010712522446800_bib32","doi-asserted-by":"crossref","first-page":"1305","DOI":"10.1167\/iovs.02-0552","article-title":"Loss of MCT1, MCT3, and MCT4 expression in the retinal pigment epithelium and neural retina of the 5A11\/basigin-null mouse","volume":"44","author":"Philp","year":"2003","journal-title":"Investigative Ophthalmology & Visual Science"},{"key":"2026010712522446800_bib33","doi-asserted-by":"crossref","first-page":"6360","DOI":"10.1073\/pnas.111583198","article-title":"CD147 facilitates HIV-1 infection by interacting with virus-associated cyclophilin A","volume":"98","author":"Pushkarsky","year":"2001","journal-title":"PNAS"},{"key":"2026010712522446800_bib34","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1007\/BF00490107","article-title":"Differential distribution of dihydrotestosterone and estradiol binding sites in the epididymis of the mouse. An autoradiographic study","volume":"81","author":"Schleicher","year":"1984","journal-title":"Histochemistry"},{"key":"2026010712522446800_bib35","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1095\/biolreprod.104.036145","article-title":"Expression of basigin, an inducer of matrix metalloproteinases, in the rat ovary","volume":"73","author":"Smedts","year":"2005","journal-title":"Biology of Reproduction"},{"key":"2026010712522446800_bib36","doi-asserted-by":"crossref","first-page":"3193","DOI":"10.1158\/0008-5472.CAN-04-3605","article-title":"Extracellular matrix metalloproteinase inducer stimulates tumor angiogenesis by elevating vascular endothelial cell growth factor and matrix metalloproteinases","volume":"65","author":"Tang","year":"2005","journal-title":"Cancer Research"},{"key":"2026010712522446800_bib37","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1046\/j.1439-0264.1999.00194.x","article-title":"Histological characterization of defective spermatogenesis in mice lacking the basigin gene","volume":"28","author":"Toyama","year":"1999","journal-title":"Anatomia, Histologia, Embryologia"},{"key":"2026010712522446800_bib38","doi-asserted-by":"crossref","first-page":"2584","DOI":"10.1093\/nar\/gkp117","article-title":"Estradiol downregulates miR-21 expression and increases miR-21 target gene expression in MCF-7 breast cancer cells","volume":"37","author":"Wickramasinghe","year":"2009","journal-title":"Nucleic Acids Research"},{"key":"2026010712522446800_bib39","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1002\/mrd.10128","article-title":"Basigin expression and hormonal regulation in mouse uterus during the peri-implantation period","volume":"63","author":"Xiao","year":"2002","journal-title":"Molecular Reproduction and Development"},{"key":"2026010712522446800_bib40","doi-asserted-by":"crossref","first-page":"10678","DOI":"10.1073\/pnas.0600507103","article-title":"A unique preovulatory expression pattern plays a key role in the physiological functions of BMP-15 in the mouse","volume":"103","author":"Yoshino","year":"2006","journal-title":"PNAS"},{"key":"2026010712522446800_bib41","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.bbrc.2006.07.207","article-title":"Human microRNA clusters: genomic organization and expression profile in leukemia cell lines","volume":"349","author":"Yu","year":"2006","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"2026010712522446800_bib42","doi-asserted-by":"crossref","first-page":"22959","DOI":"10.1074\/jbc.M201593200","article-title":"Active site residues of cyclophilin A are crucial for its signaling activity via CD147","volume":"277","author":"Yurchenko","year":"2002","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712522446800_bib43","doi-asserted-by":"crossref","first-page":"9136","DOI":"10.1073\/pnas.0508889103","article-title":"microRNAs exhibit high frequency genomic alterations in human cancer","volume":"103","author":"Zhang","year":"2006","journal-title":"PNAS"},{"key":"2026010712522446800_bib44","doi-asserted-by":"crossref","first-page":"870","DOI":"10.1002\/j.1939-4640.2002.tb02345.x","article-title":"Localization of androgen and estrogen receptors in adult male mouse reproductive tract","volume":"23","author":"Zhou","year":"2002","journal-title":"Journal of Andrology"},{"key":"2026010712522446800_bib45","doi-asserted-by":"crossref","first-page":"1921","DOI":"10.1016\/S0002-9440(10)64660-3","article-title":"Tumorigenic potential of extracellular matrix metalloproteinase inducer","volume":"158","author":"Zucker","year":"2001","journal-title":"American Journal of Pathology"}],"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rep.bioscientifica.com\/view\/journals\/rep\/139\/6\/1057.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/139\/6\/1057\/64532171\/1057.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/139\/6\/1057\/64532171\/1057.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T17:56:30Z","timestamp":1767808590000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/139\/6\/1057\/8243195"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6,1]]},"references-count":45,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,6,1]]}},"URL":"https:\/\/doi.org\/10.1530\/rep-10-0069","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2010,6,1]]},"published":{"date-parts":[[2010,6,1]]}}}