{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T16:27:32Z","timestamp":1791044852649,"version":"4.1.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2010,9,1]],"date-time":"2010-09-01T00:00:00Z","timestamp":1283299200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2010,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In this study, I examined whether sperm hyperactivation in hamster is regulated by steroid hormones such as estrogen (estradiol, E2) and progesterone. Although sperm hyperactivation was enhanced by progesterone, 17\u03b2-estradiol (17\u03b2E2) itself did not affect sperm hyperactivation. However, 17\u03b2E2 suppressed progesterone-enhanced hyperactivation in a concentration-dependent manner through non-genomic pathways when spermatozoa were exposed to 17\u03b2E2 at the same time or before exposure to progesterone. When spermatozoa were exposed to 17\u03b2E2 after exposure to progesterone, 17\u03b2E2 did not suppress progesterone-enhanced hyperactivation. Moreover, 17\u03b1-estradiol, an inactive isomer of E2, did not suppress progesterone-enhanced hyperactivation. Observations using a FITC-conjugated 17\u03b2E2 showed that it binds to the acrosome region of the sperm head. Binding of 17\u03b2E2 to spermatozoa was not inhibited by progesterone, although 17\u03b2E2 did not suppress progesterone-enhanced hyperactivation when spermatozoa were exposed to 17\u03b2E2 after exposure to progesterone. On the other hand, binding of progesterone to spermatozoa was also not inhibited by 17\u03b2E2 even if progesterone-enhanced hyperactivation was suppressed by 17\u03b2E2. Although tyrosine phosphorylations of sperm proteins were enhanced by progesterone, enhancement of tyrosine phosphorylations by progesterone was suppressed by 17\u03b2E2. Moreover, tyrosine phosphorylations were inhibited by 17\u03b2E2 when only 17\u03b2E2 was added to the medium. From these results, it is likely that 17\u03b2E2 competitively suppresses progesterone-enhanced hyperactivation through the inhibition of tyrosine phosphorylations via non-genomic pathways.<\/jats:p>","DOI":"10.1530\/rep-10-0168","type":"journal-article","created":{"date-parts":[[2010,6,20]],"date-time":"2010-06-20T20:18:18Z","timestamp":1277065098000},"page":"453-464","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":35,"title":["Suppression of progesterone-enhanced hyperactivation in hamster spermatozoa by estrogen"],"prefix":"10.1093","volume":"140","author":[{"given":"Masakatsu","family":"Fujinoki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2010,9,1]]},"reference":[{"key":"2026010712521957600_bib1","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.2741\/A345","article-title":"Nongenomic effects of progesterone on spermatozoa: mechanisms of signal transduction and clinical implications","volume":"3","author":"Baldi","year":"1998","journal-title":"Frontiers in Bioscience"},{"key":"2026010712521957600_bib2","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/S0303-7207(99)00220-8","article-title":"A novel functional estrogen receptor on human sperm membrane interferes with progesterone effects","volume":"161","author":"Baldi","year":"2000","journal-title":"Molecular and Cellular Endocrinology"},{"key":"2026010712521957600_bib3","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1016\/j.mce.2009.02.006","article-title":"Nongenomic activation of spermatozoa by steroid hormones: facts and fictions","volume":"308","author":"Baldi","year":"2009","journal-title":"Molecular and Cellular Endocrinology"},{"key":"2026010712521957600_bib4","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1006\/dbio.1996.0301","article-title":"Regulation of protein tyrosine phosphorylation in human sperm by a calcium\/calmodulin-dependent mechanism: identification of A kinase anchoring proteins as major substrates for tyrosine phosphorylation","volume":"180","author":"Carrera","year":"1996","journal-title":"Developmental Biology"},{"key":"2026010712521957600_bib5","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1530\/REP-08-0202","article-title":"Melatonin-enhanced hyperactivation of hamster sperm","volume":"136","author":"Fujinoki","year":"2008","journal-title":"Reproduction"},{"key":"2026010712521957600_bib6","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/s12522-009-0012-2","article-title":"Non-genomic regulation of mammalian sperm hyperactivation","volume":"8","author":"Fujinoki","year":"2009","journal-title":"Reproductive Medicine and Biology"},{"key":"2026010712521957600_bib7","doi-asserted-by":"crossref","first-page":"45","DOI":"10.2220\/biomedres.22.45","article-title":"Serine phosphorylation of flagellar proteins associated with the motility activation of hamster spermatozoa","volume":"22","author":"Fujinoki","year":"2001","journal-title":"Biomedical Research"},{"key":"2026010712521957600_bib8","doi-asserted-by":"crossref","first-page":"147","DOI":"10.2220\/biomedres.22.147","article-title":"Tyrosine phosphorylation and dephosphorylation associated with motility of hamster spermatozoa","volume":"22","author":"Fujinoki","year":"2001","journal-title":"Biomedical Research"},{"key":"2026010712521957600_bib9","first-page":"123","article-title":"Profiling of proteins phosphorylated or dephosphorylated during hyperactivation on hamster spermatozoa","volume":"5","author":"Fujinoki","year":"2006","journal-title":"Reproductive Medicine and Biology"},{"key":"2026010712521957600_bib10","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1083\/jcb.200210057","article-title":"Phospholipase C\u03b44 is required for Ca2+ mobilization essential for acrosome reaction in sperm","volume":"161","author":"Fukami","year":"2003","journal-title":"Journal of Cell Biology"},{"key":"2026010712521957600_bib11","doi-asserted-by":"crossref","first-page":"1327","DOI":"10.1095\/biolreprod67.4.1327","article-title":"Progesterone receptor as an indicator of sperm function","volume":"67","author":"Gadkar","year":"2002","journal-title":"Biology of Reproduction"},{"key":"2026010712521957600_bib12","doi-asserted-by":"crossref","first-page":"46315","DOI":"10.1074\/jbc.M401194200","article-title":"Stimulation of human spermatozoa with progesterone gradients to stimulate approach to the oocyte","volume":"279","author":"Harper","year":"2004","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712521957600_bib13","doi-asserted-by":"crossref","first-page":"811","DOI":"10.1095\/biolreprod62.3.811","article-title":"Involvement of protein kinase A and A kinase anchoring protein in the progesterone-initiated human sperm acrosome reaction","volume":"62","author":"Harrison","year":"2000","journal-title":"Biology of Reproduction"},{"key":"2026010712521957600_bib14","doi-asserted-by":"crossref","first-page":"1606","DOI":"10.1095\/biolreprod65.5.1606","article-title":"An inositol 1,4,5-trisphoshate receptor-gated intracellular Ca2+ store is involved in regulating sperm hyperactivated motility","volume":"65","author":"Ho","year":"2001","journal-title":"Biology of Reproduction"},{"key":"2026010712521957600_bib15","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1006\/dbio.2002.0797","article-title":"Hyperactivated motility of bull sperm is triggered at the axoneme by Ca2+ and not cAMP","volume":"250","author":"Ho","year":"2002","journal-title":"Developmental Biology"},{"key":"2026010712521957600_bib16","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1677\/joe.1.05607","article-title":"Identification of a 71\u200akDa protein as a putative non-genomic membrane progesterone receptor in boar spermatozoa","volume":"184","author":"Jang","year":"2005","journal-title":"Journal of Endocrinology"},{"key":"2026010712521957600_bib17","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1046\/j.1439-0272.2001.00410.x","article-title":"Capacitation-associated changes in protein tyrosine phosphorylation, hyperactivation and acrosome reaction in hamster spermatozoa","volume":"33","author":"Jha","year":"2001","journal-title":"Andrologia"},{"key":"2026010712521957600_bib18","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1002\/mrd.1155","article-title":"Identification of the major tyrosine phosphorylated protein of capacitated hamster spermatozoa as a homologue of mammalian sperm A kinase anchoring protein","volume":"61","author":"Jha","year":"2002","journal-title":"Molecular Reproduction and Development"},{"key":"2026010712521957600_bib19","doi-asserted-by":"crossref","first-page":"680","DOI":"10.1038\/227680a0","article-title":"Cleavage of structural proteins during assembly of the head of bacteriophage T4","volume":"227","author":"Laemmli","year":"1970","journal-title":"Nature"},{"key":"2026010712521957600_bib20","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1002\/mrd.1120120209","article-title":"A molecular membrane model of sperm capacitation and the acrosome reaction of mammalian spermatozoa","volume":"13","author":"Langlais","year":"1985","journal-title":"Gamete Research"},{"key":"2026010712521957600_bib21","doi-asserted-by":"crossref","first-page":"477","DOI":"10.1095\/biolreprod53.3.477","article-title":"Progesterone concentration in serum, follicular fluid, and oviductal fluid of the golden hamster during the periovulatory period","volume":"53","author":"Libersky","year":"1995","journal-title":"Biology of Reproduction"},{"key":"2026010712521957600_bib22","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1095\/biolreprod53.3.483","article-title":"Effects of progesterone on in vitro sperm capacitation and egg penetration in the golden hamster","volume":"53","author":"Libersky","year":"1995","journal-title":"Biology of Reproduction"},{"key":"2026010712521957600_bib23","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1002\/(SICI)1098-2795(199611)45:3<313::AID-MRD8>3.0.CO;2-V","article-title":"Studies related to progesterone-induced hamster sperm acrosome reaction","volume":"45","author":"Llanos","year":"1996","journal-title":"Molecular Reproduction and Development"},{"key":"2026010712521957600_bib24","doi-asserted-by":"crossref","first-page":"46","DOI":"10.1038\/nrm1009","article-title":"Nongenomic actions of steroid hormones","volume":"4","author":"L\u00f6sel","year":"2003","journal-title":"Nature Reviews. Molecular Cell Biology"},{"key":"2026010712521957600_bib25","doi-asserted-by":"crossref","first-page":"1670","DOI":"10.1210\/jcem.84.5.5670","article-title":"Identification and characterization of a novel functional estrogen receptor on human sperm membrane that interferes with progesterone effects","volume":"84","author":"Luconi","year":"1999","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"key":"2026010712521957600_bib26","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1016\/j.steroids.2004.05.013","article-title":"Human spermatozoa as a model for studying membrane receptors mediating rapid nongenomic effects of progesterone and estrogens","volume":"69","author":"Luconi","year":"2004","journal-title":"Steroids"},{"key":"2026010712521957600_bib27","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1447-0578.2008.00202.x","article-title":"Regulation of hyperactivation of hamster spermatozoa by progesterone","volume":"7","author":"Noguchi","year":"2008","journal-title":"Reproductive Medicine and Biology"},{"key":"2026010712521957600_bib28","doi-asserted-by":"crossref","first-page":"9699","DOI":"10.1016\/S0021-9258(17)39295-5","article-title":"Sodium bicarbonate in seminal plasma stimulates the motility of mammalian spermatozoa through the direct activation of adenylate cyclase","volume":"260","author":"Okamura","year":"1985","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712521957600_bib29","doi-asserted-by":"crossref","first-page":"828","DOI":"10.1016\/0006-291X(89)92508-4","article-title":"Steroid induced exocytosis: the human sperm acrosome reaction","volume":"160","author":"Osman","year":"1989","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"2026010712521957600_bib30","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1095\/biolreprod54.5.993","article-title":"Human sperm plasma membrane progesterone receptor(s) and the acrosome reaction","volume":"54","author":"Sabeur","year":"1996","journal-title":"Biology of Reproduction"},{"key":"2026010712521957600_bib31","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1046\/j.1439-0531.2003.00397.x","article-title":"Hyperactivated motility in sperm","volume":"38","author":"Suarez","year":"2003","journal-title":"Reproduction in Domestic Animals"},{"key":"2026010712521957600_bib32","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0015-0282(16)56051-8","article-title":"Effect of progesterone on human zona pellucida sperm binding and oocyte penetrationg capacity","volume":"60","author":"Sueldo","year":"1993","journal-title":"Fertility and Sterility"},{"key":"2026010712521957600_bib33","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1530\/REP-08-0366","article-title":"Regulation of hyperactivation by PP2A in hamster spermatozoa","volume":"139","author":"Suzuki","year":"2010","journal-title":"Reproduction"},{"key":"2026010712521957600_bib34","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1095\/biolreprod59.1.1","article-title":"Regulation of protein phosphorylation during sperm capacitation","volume":"59","author":"Visconti","year":"1998","journal-title":"Biology of Reproduction"},{"key":"2026010712521957600_bib35","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1242\/dev.121.4.1129","article-title":"Capacitation of mouse spermatozoa I. Correlation between the capacitation state and protein tyrosine phosphorylation","volume":"121","author":"Visconti","year":"1995","journal-title":"Development"},{"key":"2026010712521957600_bib36","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1002\/j.1939-4640.1998.tb01994.x","article-title":"The molecular basis of capacitation","volume":"19","author":"Visconti","year":"1998","journal-title":"Journal of Andrology"},{"key":"2026010712521957600_bib37"},{"key":"2026010712521957600_bib38","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1073\/pnas.91.2.529","article-title":"Progesterone and RU486: opposing effects on human sperm","volume":"91","author":"Yang","year":"1994","journal-title":"PNAS"},{"key":"2026010712521957600_bib39","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1002\/mrd.1120200103","article-title":"Ultrastructural studies of the early events of the human sperm acrosome reaction as initiated by human follicular fluid","volume":"20","author":"Yudin","year":"1988","journal-title":"Gamete Research"}],"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rep.bioscientifica.com\/view\/journals\/rep\/140\/3\/453.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/140\/3\/453\/64530181\/453.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/140\/3\/453\/64530181\/453.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T17:56:22Z","timestamp":1767808582000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/140\/3\/453\/8243190"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,9,1]]},"references-count":39,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2010,9,1]]}},"URL":"https:\/\/doi.org\/10.1530\/rep-10-0168","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2010,9,1]]},"published":{"date-parts":[[2010,9,1]]}}}