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Moreover, net n-6 PUFA-enriched HDL reduced early embryo development, but in the absence of a net uptake of FA. Consequently, we hypothesised that a) effects of n-3 PUFA on ovarian steroidogenesis are mediated by theca rather than GCs and b) during embryo culture lipids are acquired solely from the albumin fraction of serum, so that albumin-delivered n-3 and n-6 PUFA exert a greater differential effect on embryo development than either low-density lipoprotein (LDL)- or HDL-delivered PUFA. Data confirmed that n-3 PUFA increases P4 production solely in theca cells and that this is associated with an increase in STAR transcript expression. Furthermore, LDL- and HDL-delivered n-3 PUFA are equally efficacious in this regard during the first 96\u200ah of culture, but thereafter only HDL-delivered n-3 PUFA induces this effect in partially luteinised theca cells. We also demonstrate that albumin is the sole serum fraction that leads to a net uptake of FA during embryo culture. PUFA-enriched serum and albumin increased the yield of morphologically poorer quality blastocysts with increased transcript expression for the antioxidant enzyme SOD1. Important differential effects of n-3 and n-6 PUFA on ovarian steroidogenesis acting solely on theca cells are identified, but differential effects of PUFA on embryo development are less apparent.<\/jats:p>","DOI":"10.1530\/rep-10-0337","type":"journal-article","created":{"date-parts":[[2010,11,2]],"date-time":"2010-11-02T22:42:22Z","timestamp":1288737742000},"page":"105-118","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":56,"title":["Effects of omega-3 and -6 polyunsaturated fatty acids on ovine follicular cell steroidogenesis, embryo development and molecular markers of fatty acid metabolism"],"prefix":"10.1093","volume":"141","author":[{"given":"Jaime","family":"Hughes","sequence":"first","affiliation":[{"name":"Schools of Biosciences 2Veterinary Medicine and Science University of Nottingham, Sutton Bonington Campus, Leicestershire LE12 5RD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wing Yee","family":"Kwong","sequence":"additional","affiliation":[{"name":"Schools of Biosciences 2Veterinary Medicine and Science University of Nottingham, Sutton Bonington Campus, Leicestershire LE12 5RD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongfang","family":"Li","sequence":"additional","affiliation":[{"name":"Schools of Biosciences 2Veterinary Medicine and Science University of Nottingham, Sutton Bonington Campus, Leicestershire LE12 5RD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew M","family":"Salter","sequence":"additional","affiliation":[{"name":"Schools of Biosciences 2Veterinary Medicine and Science University of Nottingham, Sutton Bonington Campus, Leicestershire LE12 5RD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard G","family":"Lea","sequence":"additional","affiliation":[{"name":"Schools of Biosciences 2Veterinary Medicine and Science University of Nottingham, Sutton Bonington Campus, 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