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The objectives of this study were to determine i) the survival time of ectopically transplanted allogeneic trophoblast cells in non-pregnant recipient mares, ii) whether equine chorionic gonadotropin (eCG) can be delivered systemically by transplanted chorionic girdle cells, and iii) whether eCG delivered by the transplanted cells is biologically active and can suppress behavioral signs associated with estrus. Ectopically transplanted chorionic girdle survived for up to 105 days with a mean lifespan of 75 days (95% confidence interval 55\u201394) and secreted sufficient eCG for the hormone to be measurable in the recipients\u2019 circulation. Immunohistochemical labeling of serial biopsies of the transplant sites and measurement of eCG profiles demonstrated that graft survival was similar to the lifespan of equine endometrial cups in normal horse pregnancy. The eCG secreted by the transplanted cells induced corpora lutea formation and sustained systemic progesterone levels in the recipient mares, effects that are also observed during pregnancy. This in turn caused suppression of estrus behavior in the recipients for up to 3 months. Thus, ectopically transplanted equine trophoblast provides an unusual example of sustained viability and function of an immunogenic transplant in a recipient with an intact immune system. This model highlights the importance of innate immunoregulatory capabilities of invasive trophoblast cells and describes a new method to deliver sustained circulating concentrations of eCG in non-pregnant mares.<\/jats:p>","DOI":"10.1530\/rep-10-0462","type":"journal-article","created":{"date-parts":[[2011,3,9]],"date-time":"2011-03-09T22:20:28Z","timestamp":1299709228000},"page":"849-856","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":11,"title":["Functions of ectopically transplanted invasive horse trophoblast"],"prefix":"10.1093","volume":"141","author":[{"given":"Amanda M","family":"de Mestre","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Hanlon","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Baker Institute for Animal Health, Cornell University, Ithaca, New York 14853, USA 1Matamata Veterinary Services Matamata 3400, New Zealand 2Department of Population Medicine and Diagnostic Sciences College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA 3Paul Mellon Laboratory of Equine Reproduction 18 Woodditton Road, Newmarket, Suffolk, CB8 9BJ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A Paige","family":"Adams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erin","family":"Runcan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane C","family":"Leadbeater","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hollis N","family":"Erb","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Baker Institute for Animal Health, Cornell University, Ithaca, New York 14853, USA 1Matamata Veterinary Services Matamata 3400, New Zealand 2Department of Population Medicine and Diagnostic Sciences College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA 3Paul Mellon Laboratory of Equine Reproduction 18 Woodditton Road, Newmarket, Suffolk, CB8 9BJ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina C","family":"Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W R","family":"Allen","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine Baker Institute for Animal Health, Cornell University, Ithaca, New York 14853, USA 1Matamata Veterinary Services Matamata 3400, New Zealand 2Department of Population Medicine and Diagnostic Sciences College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA 3Paul Mellon Laboratory of Equine Reproduction 18 Woodditton Road, Newmarket, Suffolk, CB8 9BJ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas F","family":"Antczak","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2011,6,1]]},"reference":[{"key":"2026010712543196200_bib1","doi-asserted-by":"crossref","first-page":"753","DOI":"10.1095\/biolreprod64.3.753","article-title":"Ectopic transplantation of equine invasive trophoblast","volume":"64","author":"Adams","year":"2001","journal-title":"Biology of Reproduction"},{"key":"2026010712543196200_bib2","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.theriogenology.2007.04.058","article-title":"The effect of skin allografting on the equine endometrial cup reaction","volume":"68","author":"Adams","year":"2007","journal-title":"Theriogenology"},{"key":"2026010712543196200_bib3","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1677\/joe.0.0430593","article-title":"The immunological measurement of pregnant mare serum gonadotrophin","volume":"43","author":"Allen","year":"1969","journal-title":"Journal of Endocrinology"},{"key":"2026010712543196200_bib4","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1530\/jrf.0.0290313","article-title":"The origin of the equine endometrial cups. 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