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However, in terms of male reproductive function, beyond erectile dysfunction, little is known about the influence of this cohort (collectively or separately) on spermatogenesis and sperm quality. The aims of this study were to assess the reproductive tract of a MetS animal model for detrimental changes, to determine whether a group of compounds (advanced glycation end products and their receptor) known to cause cell dysfunction and DNA damage was present and assess whether hypogonadotropic hypogonadism was the main contributing factor for the changes seen. Animals fed a high-fat diet were found to have significantly increased cholesterol, triglycerides, blood glucose, mean arterial pressure and visceral fat levels. Although serum testosterone was decreased, no changes were seen in either testicular or epididymal histology. Immunolocalisation of N\u03f5-carboxymethyl-lysine and the receptor for advanced glycation end products was found in the testes, epididymides and sperm of the two treated groups of animals; however, ELISA did not show any difference in protein levels. Similarly, assessment of sperm nuclear DNA (nDNA) fragmentation by acridine orange test did not find significant differences in nDNA integrity. We conclude that the minimal effect on spermatogenesis and sperm quality seen in our model is probably due to the moderate increase of blood glucose rather than the hypogonadism.<\/jats:p>","DOI":"10.1530\/rep-10-0472","type":"journal-article","created":{"date-parts":[[2011,4,4]],"date-time":"2011-04-04T22:29:47Z","timestamp":1301956187000},"page":"63-71","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":35,"title":["Spermatogenic and sperm quality differences in an experimental model of metabolic syndrome and hypogonadal hypogonadism"],"prefix":"10.1093","volume":"142","author":[{"given":"Con","family":"Mallidis","sequence":"first","affiliation":[{"name":"Centre of Reproductive Medicine and Andrology University of M\u00fcnster, Domagkstrasse 11, D-48149 Munster, Germany 2Department of Obstetrics and Gynaecology Queen's University Belfast, Belfast, BT12 6BJ, UK 3Andrology and Sexual Medicine Unit Department of Clinical Physiopathology, University of Florence, Florence 50139, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agnieszka","family":"Czerwiec","sequence":"additional","affiliation":[{"name":"Centre of Reproductive Medicine and Andrology University of M\u00fcnster, Domagkstrasse 11, D-48149 Munster, Germany 2Department of Obstetrics and Gynaecology Queen's University Belfast, Belfast, BT12 6BJ, UK 3Andrology and Sexual Medicine Unit Department of Clinical Physiopathology, University of Florence, Florence 50139, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Filippi","sequence":"additional","affiliation":[{"name":"Centre of Reproductive Medicine and Andrology University of M\u00fcnster, Domagkstrasse 11, D-48149 Munster, Germany 2Department of Obstetrics and Gynaecology Queen's University Belfast, Belfast, BT12 6BJ, UK 3Andrology and Sexual Medicine Unit Department of Clinical Physiopathology, University of Florence, Florence 50139, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"O'Neill","sequence":"additional","affiliation":[{"name":"Centre of Reproductive Medicine and Andrology University of M\u00fcnster, Domagkstrasse 11, D-48149 Munster, Germany 2Department of Obstetrics and Gynaecology Queen's University Belfast, Belfast, BT12 6BJ, UK 3Andrology and Sexual Medicine Unit Department of Clinical Physiopathology, University of Florence, Florence 50139, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Maggi","sequence":"additional","affiliation":[{"name":"Centre of Reproductive Medicine and Andrology University of M\u00fcnster, Domagkstrasse 11, D-48149 Munster, Germany 2Department of Obstetrics and Gynaecology Queen's University Belfast, Belfast, BT12 6BJ, UK 3Andrology and Sexual Medicine Unit Department of Clinical Physiopathology, University of Florence, Florence 50139, 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