{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T18:47:38Z","timestamp":1791139658091,"version":"4.1.0"},"reference-count":78,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2011,7,1]],"date-time":"2011-07-01T00:00:00Z","timestamp":1309478400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2011,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The present study investigated the role of IGF1 in lactating lean and non-lactating obese dairy cows by injecting 1\u200a\u03bcg IGF1 into the ovaries prior to superovulation. This amount of IGF1 has been linked with pregnancy loss in women with the polycystic ovary syndrome (PCOS) and was associated with impaired bovine oocyte competence in vitro. Transcript abundance and protein expression of selected genes involved in apoptosis, glucose metabolism, and the IGF system were analyzed. Plasma concentrations of IGF1 and leptin, and IGF1 in uterine luminal fluid (ULF), were also measured. IGF1 treatment decreased embryo viability in lean cows to the levels observed in obese cows. Obese cows were not affected by IGF1 treatment and showed elevated levels of IGF1 (in both plasma and ULF) and leptin. Blastocysts from lean cows treated with IGF1 showed a higher abundance of SLC2A1 and IGFBP3 transcripts. IGF1 treatment reduced protein expression of tumor protein 53 in blastocysts of lean cows, whereas the opposite was observed in obese cows. IGF1 in plasma and ULF was correlated only in the control groups. Blastocyst transcript abundance of IGF1 receptor and IGFBP3 correlated positively with IGF1 concentrations in both plasma and ULF in lean cows. The detrimental microenvironment created by IGF1 injection in lean cows and the lack of effect in obese cows resemble to a certain extent the situation observed in PCOS patients, where IGF1 bioavailability is increased in normal-weight women but reduced in obese women, suggesting that this bovine model could be useful for studying IGF1 involvement in PCOS.<\/jats:p>","DOI":"10.1530\/rep-10-0512","type":"journal-article","created":{"date-parts":[[2011,5,4]],"date-time":"2011-05-04T23:41:15Z","timestamp":1304552475000},"page":"41-52","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":24,"title":["<i>In vivo<\/i>\n                    oocyte developmental competence is reduced in lean but not in obese superovulated dairy cows after intraovarian administration of IGF1"],"prefix":"10.1093","volume":"142","author":[{"given":"Miguel A","family":"Velazquez","sequence":"first","affiliation":[{"name":"Department of Biotechnology Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), H\u00f6ltystrasse 10 Mariensee, 31535 Neustadt, Germany 2Escuela Superior de Ciencias Agropecuarias Universidad Aut\u00f3noma de Campeche, Calle 53s\/n, C.P. 24350 Esc\u00e1rcega, Campeche, Mexico 3Physiology Weihenstephan Technische Universit\u00e4t M\u00fcnchen, Weihenstephaner Berg 3, 85354 Freising, Germany 4Laboratory of Endocrinology and Animal Reproduction Faculty of Veterinary Medicine, University of Liege, B-4000 Liege, Belgium 5Physiology and Hygiene Unit Institute for Animal Science, University of Bonn, Katzenburgweg 7-9, 53115 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus-Gerd","family":"Hadeler","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), H\u00f6ltystrasse 10 Mariensee, 31535 Neustadt, Germany 2Escuela Superior de Ciencias Agropecuarias Universidad Aut\u00f3noma de Campeche, Calle 53s\/n, C.P. 24350 Esc\u00e1rcega, Campeche, Mexico 3Physiology Weihenstephan Technische Universit\u00e4t M\u00fcnchen, Weihenstephaner Berg 3, 85354 Freising, Germany 4Laboratory of Endocrinology and Animal Reproduction Faculty of Veterinary Medicine, University of Liege, B-4000 Liege, Belgium 5Physiology and Hygiene Unit Institute for Animal Science, University of Bonn, Katzenburgweg 7-9, 53115 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris","family":"Herrmann","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), H\u00f6ltystrasse 10 Mariensee, 31535 Neustadt, Germany 2Escuela Superior de Ciencias Agropecuarias Universidad Aut\u00f3noma de Campeche, Calle 53s\/n, C.P. 24350 Esc\u00e1rcega, Campeche, Mexico 3Physiology Weihenstephan Technische Universit\u00e4t M\u00fcnchen, Weihenstephaner Berg 3, 85354 Freising, Germany 4Laboratory of Endocrinology and Animal Reproduction Faculty of Veterinary Medicine, University of Liege, B-4000 Liege, Belgium 5Physiology and Hygiene Unit Institute for Animal Science, University of Bonn, Katzenburgweg 7-9, 53115 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilfried A","family":"Kues","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), H\u00f6ltystrasse 10 Mariensee, 31535 Neustadt, Germany 2Escuela Superior de Ciencias Agropecuarias Universidad Aut\u00f3noma de Campeche, Calle 53s\/n, C.P. 24350 Esc\u00e1rcega, Campeche, Mexico 3Physiology Weihenstephan Technische Universit\u00e4t M\u00fcnchen, Weihenstephaner Berg 3, 85354 Freising, Germany 4Laboratory of Endocrinology and Animal Reproduction Faculty of Veterinary Medicine, University of Liege, B-4000 Liege, Belgium 5Physiology and Hygiene Unit Institute for Animal Science, University of Bonn, Katzenburgweg 7-9, 53115 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Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), H\u00f6ltystrasse 10 Mariensee, 31535 Neustadt, Germany 2Escuela Superior de Ciencias Agropecuarias Universidad Aut\u00f3noma de Campeche, Calle 53s\/n, C.P. 24350 Esc\u00e1rcega, Campeche, Mexico 3Physiology Weihenstephan Technische Universit\u00e4t M\u00fcnchen, Weihenstephaner Berg 3, 85354 Freising, Germany 4Laboratory of Endocrinology and Animal Reproduction Faculty of Veterinary Medicine, University of Liege, B-4000 Liege, Belgium 5Physiology and Hygiene Unit Institute for Animal Science, University of Bonn, Katzenburgweg 7-9, 53115 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beno\u00eet","family":"R\u00e9my","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), H\u00f6ltystrasse 10 Mariensee, 31535 Neustadt, Germany 2Escuela Superior de Ciencias Agropecuarias Universidad Aut\u00f3noma de Campeche, Calle 53s\/n, C.P. 24350 Esc\u00e1rcega, Campeche, Mexico 3Physiology Weihenstephan Technische Universit\u00e4t M\u00fcnchen, Weihenstephaner Berg 3, 85354 Freising, Germany 4Laboratory of Endocrinology and Animal Reproduction Faculty of Veterinary Medicine, University of Liege, B-4000 Liege, Belgium 5Physiology and Hygiene Unit Institute for Animal Science, University of Bonn, Katzenburgweg 7-9, 53115 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Fran\u00e7ois","family":"Beckers","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), H\u00f6ltystrasse 10 Mariensee, 31535 Neustadt, Germany 2Escuela Superior de Ciencias Agropecuarias Universidad Aut\u00f3noma de Campeche, Calle 53s\/n, C.P. 24350 Esc\u00e1rcega, Campeche, Mexico 3Physiology Weihenstephan Technische Universit\u00e4t M\u00fcnchen, Weihenstephaner Berg 3, 85354 Freising, Germany 4Laboratory of Endocrinology and Animal Reproduction Faculty of Veterinary Medicine, University of Liege, B-4000 Liege, Belgium 5Physiology and Hygiene Unit Institute for Animal Science, University of Bonn, Katzenburgweg 7-9, 53115 Bonn, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helga","family":"Sauerwein","sequence":"additional","affiliation":[{"name":"Department of Biotechnology Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), H\u00f6ltystrasse 10 Mariensee, 31535 Neustadt, Germany 2Escuela Superior de Ciencias Agropecuarias Universidad Aut\u00f3noma de Campeche, Calle 53s\/n, C.P. 24350 Esc\u00e1rcega, Campeche, Mexico 3Physiology Weihenstephan Technische Universit\u00e4t M\u00fcnchen, Weihenstephaner Berg 3, 85354 Freising, Germany 4Laboratory of Endocrinology and Animal Reproduction Faculty of Veterinary Medicine, University of Liege, B-4000 Liege, Belgium 5Physiology and Hygiene Unit Institute for Animal Science, University of Bonn, 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