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Sequences represented in the micrococcal nuclease digestion solubilised (MNDS) but not the MND insoluble (MNDI) chromatin are strongly enriched in chromosomal regions of high gene density. Furthermore, by fluorescence in situ hybridisation (FISH) analysis, we show that MNDS and MNDI DNAs occupy distinct domains of decondensed mouse sperm nuclei that may also retain abundant histones. More detailed in silico analysis of CGH probe location in relation to known promoters and sequences recognised by CCCTC binding factor (CTCF) shows a significant excess of both in MNDS chromatin. A functional analysis of gene promoters reveals strong ontological signatures for ion transport on methylated promoters associated with CTCF binding sequences in MNDS chromatin. Sensory perception is the only strong ontological signature present in MNDI chromatin, driven by promoters that are not associated with CTCF regardless of their methylation status.<\/jats:p>","DOI":"10.1530\/rep-10-0536","type":"journal-article","created":{"date-parts":[[2011,4,21]],"date-time":"2011-04-21T22:38:05Z","timestamp":1303425485000},"page":"73-86","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":14,"title":["Key gene regulatory sequences with distinctive ontological signatures associate with differentially endonuclease-accessible mouse sperm chromatin"],"prefix":"10.1093","volume":"142","author":[{"given":"Myriam","family":"Saida","sequence":"first","affiliation":[{"name":"Leeds Institute of Genetics Health and Therapeutics, University of Leeds, Leeds, LS2 9JT, UK 2Faculty of Biological Sciences Institute for Integrative and Comparative Biology, University of Leeds, Leeds, LS2 9JT, UK 3Biomedical Sciences School of Life Sciences, University of Bradford, Richmond Building, Bradford, BD7 1DP, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Iles","sequence":"additional","affiliation":[{"name":"Leeds Institute of Genetics Health and Therapeutics, University of Leeds, Leeds, LS2 9JT, UK 2Faculty of Biological Sciences Institute for Integrative and Comparative Biology, University of Leeds, Leeds, LS2 9JT, UK 3Biomedical Sciences School of Life Sciences, University of Bradford, Richmond Building, Bradford, BD7 1DP, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdul","family":"Elnefati","sequence":"additional","affiliation":[{"name":"Leeds Institute of Genetics Health and Therapeutics, University of Leeds, Leeds, LS2 9JT, UK 2Faculty of Biological Sciences Institute for Integrative and Comparative Biology, University of Leeds, Leeds, LS2 9JT, UK 3Biomedical Sciences School of Life Sciences, University of Bradford, Richmond Building, Bradford, 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