{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:49:10Z","timestamp":1792712950985,"version":"4.5.0"},"reference-count":57,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2012,6,1]],"date-time":"2012-06-01T00:00:00Z","timestamp":1338508800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2012,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Artificial insemination (AI) with sex-sorted frozen\u2013thawed spermatozoa has led to enhanced management of ex situ bottlenose dolphin populations. Extended distance of animals from the sorting facility can be overcome by the use of frozen\u2013thawed, sorted and recryopreserved spermatozoa. Although one bottlenose dolphin calf had been born using sexed frozen\u2013thawed spermatozoa derived from frozen semen, a critical evaluation of in vitro sperm quality is needed to justify the routine use of such samples in AI programs. Sperm motility parameters and plasma membrane integrity were influenced by stage of the sex-sorting process, sperm type (non-sorted and sorted) and freezing method (straw and directional) (P&amp;lt;0.05). After recryopreservation, sorted spermatozoa frozen with the directional freezing method maintained higher (P&amp;lt;0.05) motility parameters over a 24-h incubation period compared to spermatozoa frozen using straws. Quality of sperm DNA of non-sorted spermatozoa, as assessed by the sperm chromatin structure assay (SCSA), was high and remained unchanged throughout freeze\u2013thawing and incubation processes. Though a possible interaction between Hoechst 33342 and the SCSA-derived acridine orange was observed in stained and sorted samples, the proportion of sex-sorted, recryopreserved spermatozoa exhibiting denatured DNA was low (6.6\u00b14.1%) at 6\u200ah after the second thawing step and remained unchanged (P&amp;gt;0.05) at 24\u200ah. The viability of sorted spermatozoa was higher (P&amp;lt;0.05) than that of non-sorted spermatozoa across all time points after recryopreservation. Collective results indicate that bottlenose dolphin spermatozoa undergoing cryopreservation, sorting and recryopreservation are of adequate quality for use in AI.<\/jats:p>","DOI":"10.1530\/rep-11-0490","type":"journal-article","created":{"date-parts":[[2012,3,27]],"date-time":"2012-03-27T21:12:41Z","timestamp":1332882761000},"page":"799-813","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":21,"title":["Evaluation of motility, membrane status and DNA integrity of frozen\u2013thawed bottlenose dolphin (\n                    <i>Tursiops truncatus<\/i>\n                    ) spermatozoa after sex-sorting and recryopreservation"],"prefix":"10.1093","volume":"143","author":[{"given":"G A","family":"Montano","sequence":"first","affiliation":[{"name":"SeaWorld and Busch Gardens Reproductive Research Center SeaWorld Parks and Entertainment, San Diego, California 92109, USA, Departments of 2Veterinary Physiology and Pharmacology and 3Large Animal Clinical Sciences College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas 77843, USA and 4Faculty of Veterinary Science University of Sydney, Sydney, New South Wales 2006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D C","family":"Kraemer","sequence":"additional","affiliation":[{"name":"SeaWorld and Busch Gardens Reproductive Research Center SeaWorld Parks and Entertainment, San Diego, California 92109, USA, Departments of 2Veterinary Physiology and Pharmacology and 3Large Animal Clinical Sciences College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas 77843, USA and 4Faculty of Veterinary Science University of Sydney, Sydney, New South Wales 2006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C C","family":"Love","sequence":"additional","affiliation":[{"name":"SeaWorld and Busch Gardens Reproductive Research Center SeaWorld Parks and Entertainment, San Diego, California 92109, USA, Departments of 2Veterinary Physiology and Pharmacology and 3Large Animal Clinical Sciences College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas 77843, USA and 4Faculty of Veterinary Science University of Sydney, Sydney, New South Wales 2006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T R","family":"Robeck","sequence":"additional","affiliation":[{"name":"SeaWorld and Busch Gardens Reproductive Research Center SeaWorld Parks and Entertainment, San Diego, California 92109, USA, Departments of 2Veterinary Physiology and Pharmacology and 3Large Animal Clinical Sciences College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas 77843, USA and 4Faculty of Veterinary Science University of Sydney, Sydney, New South Wales 2006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J K","family":"O'Brien","sequence":"additional","affiliation":[{"name":"SeaWorld and Busch Gardens Reproductive Research Center SeaWorld Parks and Entertainment, San Diego, California 92109, USA, Departments of 2Veterinary Physiology and Pharmacology and 3Large Animal Clinical Sciences College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, Texas 77843, USA and 4Faculty of Veterinary Science University of Sydney, Sydney, New South Wales 2006, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,6,1]]},"reference":[{"key":"2026010712572409100_bib1","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1111\/j.1365-2605.2005.00531.x","article-title":"Impact of radio frequency electromagnetic radiation on DNA integrity in the male germline","volume":"28","author":"Aitken","year":"2005","journal-title":"International Journal of Andrology"},{"key":"2026010712572409100_bib2","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1016\/S0303-7207(01)00700-6","article-title":"New trends in gamete's cryopreservation","volume":"187","author":"Arav","year":"2002","journal-title":"Molecular and Cellular Endocrinology"},{"key":"2026010712572409100_bib3","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1002\/j.1939-4640.1988.tb01020.x","article-title":"The sperm chromatin structure assay. 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