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Here, we analyzed DNA methylation in three developmentally important, nonimprinted genes (SLC2A1, PRDX1, ZAR1) and two satellite sequences, i.e. \u2018bovine testis satellite I\u2019 (BTS) and \u2018Bos taurus alpha satellite I\u2019 (BT\u03b1S). In parallel, mRNA expression of the genes was determined by quantitative real-time PCR. Oocytes were retrieved from prepubertal calves and adult cows twice per week over a 3-week period by ultrasound-guided follicular aspiration after treatment with FSH and\/or IGF1. Both immature and in vitro matured prepubertal and adult oocytes showed a distinct hypomethylation profile of the three genes without differences between the two types of donors. The methylation status of the BTS sequence changed according to the age and treatment while the methylation status of BT\u03b1S sequence remained largely unchanged across the different age and treatment groups. Relative transcript abundance of the selected genes was significantly different in immature and in vitro matured oocytes; only minor changes related to origin and treatment were observed. In conclusion, methylation levels of the investigated satellite sequences were high (&amp;gt;50%) in all groups and showed significant variation depending on the age, treatment, or in vitro maturation. To what extent this is involved in the acquisition of developmental competence of bovine oocytes needs further study.<\/jats:p>","DOI":"10.1530\/rep-12-0134","type":"journal-article","created":{"date-parts":[[2012,6,26]],"date-time":"2012-06-26T14:29:38Z","timestamp":1340720978000},"page":"319-330","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":33,"title":["DNA methylation and mRNA expression profiles in bovine oocytes derived from prepubertal and adult donors"],"prefix":"10.1093","volume":"144","author":[{"given":"Mike","family":"Diederich","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Hansmann","sequence":"additional","affiliation":[{"name":"Institute of Farm Animal Genetics (FLI) 31535 Mariensee, Neustadt, Germany 1Institute of Human Genetics Julius Maximilians University, 97074 Wuerzburg, Germany and 2Max Planck Institute for Plant Breeding Research 50829 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Heinzmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brigitte","family":"Barg-Kues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris","family":"Herrmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Aldag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Baulain","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Reinhard","sequence":"additional","affiliation":[{"name":"Institute of Farm Animal Genetics (FLI) 31535 Mariensee, Neustadt, Germany 1Institute of Human Genetics Julius Maximilians University, 97074 Wuerzburg, Germany and 2Max Planck Institute for Plant Breeding Research 50829 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilfried","family":"Kues","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Wei\u00dfgerber","sequence":"additional","affiliation":[{"name":"Institute of Farm Animal Genetics (FLI) 31535 Mariensee, Neustadt, Germany 1Institute of Human Genetics Julius Maximilians University, 97074 Wuerzburg, Germany and 2Max Planck Institute for Plant Breeding Research 50829 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Haaf","sequence":"additional","affiliation":[{"name":"Institute of Farm Animal Genetics (FLI) 31535 Mariensee, Neustadt, Germany 1Institute of Human Genetics Julius Maximilians University, 97074 Wuerzburg, Germany and 2Max Planck Institute for Plant Breeding Research 50829 Cologne, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heiner","family":"Niemann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2012,9,1]]},"reference":[{"key":"2026010712575839000_bib1","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1095\/biolreprod.109.082164","article-title":"Effects of low methyl donor levels in culture medium during mouse follicle culture on oocyte imprinting establishment","volume":"83","author":"Anckaert","year":"2010","journal-title":"Biology of Reproduction"},{"key":"2026010712575839000_bib2","doi-asserted-by":"crossref","first-page":"11995","DOI":"10.1073\/pnas.90.24.11995","article-title":"Number of CpG islands and genes in human and mouse","volume":"90","author":"Antequera","year":"1993","journal-title":"PNAS"},{"key":"2026010712575839000_bib3","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1002\/mrd.20140","article-title":"Expression pattern of the maternal factor zygote arrest 1 (Zar1) in bovine tissues, oocytes, and embryos","volume":"69","author":"Brevini","year":"2004","journal-title":"Molecular Reproduction and Development"},{"key":"2026010712575839000_bib4","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1002\/jez.1401580106","article-title":"Studies on the development of mouse embryos in vitro. 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