{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T21:04:40Z","timestamp":1791925480856,"version":"4.3.4"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2013,8,1]],"date-time":"2013-08-01T00:00:00Z","timestamp":1375315200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Artificial insemination with frozen semen allows affordable, worldwide dissemination of gametes with superior genetics. Nevertheless, sperm are damaged by the cryopreservation process. Elucidating the molecular effects of cryopreservation on sperm could suggest methods for improving fertility of frozen\/thawed semen. This study was undertaken to examine the effect of cryopreservation on the coating of sperm by binder of sperm (BSP) proteins in seminal plasma. BSP proteins are secreted by the seminal vesicles and coat the surface of sperm by partially intercalating into the outer leaflet of the sperm plasma membrane. The BSP proteins are known to play roles in the formation of the oviductal sperm storage reservoir and in sperm capacitation. We investigated the effects of cryopreservation on the sperm BSP protein coat using Bovipure to separate live sperm from extended semen and then assaying the amounts of BSP proteins on sperm using quantitative western blotting with custom-made antibodies against unique sequences of each BSP protein. Greater amounts of all three BSP proteins (BSP1, BSP3, and BSP5) were detected on frozen\/thawed sperm than on fresh sperm. Furthermore, the reduction of BSP3 from 15 to 13\u200akDa in mass, which occurs during incubation of sperm under mild capacitating conditions, was enhanced by cryopreservation. We concluded that freezing alters the BSP protein coating on sperm, which could account in part for reduced fertility of cryopreserved semen samples.<\/jats:p>","DOI":"10.1530\/rep-12-0468","type":"journal-article","created":{"date-parts":[[2013,6,6]],"date-time":"2013-06-06T00:49:42Z","timestamp":1370479782000},"page":"111-117","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":38,"title":["Cryopreservation increases coating of bull sperm by seminal plasma binder of sperm proteins BSP1, BSP3, and BSP5"],"prefix":"10.1093","volume":"146","author":[{"given":"Florencia","family":"Ardon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan S","family":"Suarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2013,8,1]]},"reference":[{"key":"2026010712254790000_bib1","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.anireprosci.2004.12.014","article-title":"Isolation and characterization of heparin and gelatin binding buffalo seminal plasma proteins and their effect on cauda epididymal spermatozoa","volume":"90","author":"Arangasamy","year":"2005","journal-title":"Animal Reproduction Science"},{"key":"2026010712254790000_bib2","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1530\/REP-07-0333","article-title":"Chromatin-unstable boar spermatozoa have little chance of reaching oocytes in vivo","volume":"135","author":"Ard\u00f3n","year":"2008","journal-title":"Reproduction"},{"key":"2026010712254790000_bib3","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1006\/cryo.1993.1048","article-title":"Ca2+ regulation by cryopreserved bull spermatozoa in response to A23187","volume":"30","author":"Bailey","year":"1993","journal-title":"Cryobiology"},{"key":"2026010712254790000_bib4","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1002\/j.1939-4640.2000.tb03268.x","article-title":"Semen cryopreservation in domestic animals: a damaging and capacitating phenomenon","volume":"21","author":"Bailey","year":"2000","journal-title":"Journal of Andrology"},{"key":"2026010712254790000_bib5","doi-asserted-by":"crossref","first-page":"539","DOI":"10.2164\/jandrol.04172","article-title":"Immunocytochemical localization and biochemical characterization of two seminal plasma proteins that protect ram spermatozoa against cold shock","volume":"26","author":"Barrios","year":"2005","journal-title":"Journal of Andrology"},{"key":"2026010712254790000_bib6","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1002\/mrd.20565","article-title":"New insights towards understanding the mechanisms of sperm protection by egg yolk and milk","volume":"73","author":"Bergeron","year":"2006","journal-title":"Molecular Reproduction and Development"},{"key":"2026010712254790000_bib7","doi-asserted-by":"crossref","first-page":"461","DOI":"10.1002\/mrd.20310","article-title":"Isolation and characterization of the major proteins of ram seminal plasma","volume":"71","author":"Bergeron","year":"2005","journal-title":"Molecular Reproduction and Development"},{"key":"2026010712254790000_bib8","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1095\/biolreprod.106.058248","article-title":"Milk caseins decrease the binding of the major bovine seminal plasma proteins to sperm and prevent lipid loss from the sperm membrane during sperm storage","volume":"77","author":"Bergeron","year":"2007","journal-title":"Biology of Reproduction"},{"key":"2026010712254790000_bib9","doi-asserted-by":"crossref","first-page":"1312","DOI":"10.1016\/j.theriogenology.2012.05.029","article-title":"Osmotic tolerance and intracellular ion concentrations of bovine sperm are affected by cryopreservation","volume":"78","author":"Bl\u00e4sse","year":"2012","journal-title":"Theriogenology"},{"key":"2026010712254790000_bib10","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1095\/biolreprod.103.023069","article-title":"Isolation and characterization of gelatin-binding bison seminal vesicle secretory proteins","volume":"70","author":"Boisvert","year":"2004","journal-title":"Biology of Reproduction"},{"key":"2026010712254790000_bib11","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/S0014-5793(96)01310-5","article-title":"The primary structure of BSP-30K, a major lipid-, gelatin-, and heparin-binding glycoprotein of bovine seminal plasma","volume":"399","author":"Calvete","year":"1996","journal-title":"FEBS Letters"},{"key":"2026010712254790000_bib12","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1016\/S0014-5793(97)00344-X","article-title":"Isolation and characterization of heparin- and phosphorylcholine-binding proteins of boar and stallion seminal plasma. 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