{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T08:30:51Z","timestamp":1767861051150,"version":"3.49.0"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2013,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Placental oxidative stress plays a key role in the pathophysiology of several placenta-related disorders. Oxidative stress occurs when excess reactive oxygen species (ROS) damages cellular components, an outcome limited by antioxidant enzymes; mitochondrial uncoupling protein 2 (UCP2) also limits ROS production. We recently reported that maternal dietary omega-3 polyunsaturated fatty acid (n-3 PUFA) supplementation reduced placental oxidative damage and enhanced fetal and placental growth in the rats. Here, we examined the effect of n-3 PUFAs on placental antioxidant defences and whether n-3 PUFA supplementation could prevent growth restriction induced by placental ischaemia\u2013reperfusion (IR), a known inducer of oxidative stress. Rats were fed either standard or high-n-3 PUFA diets from day 1 of pregnancy. Placentas were collected on days 17 and 22 in untreated pregnancies (term=day 23) and at day 22 following IR treatment on day 17. Expression of several antioxidant enzyme genes (Sod1, Sod2, Sod3, Cat, Txn1 and Gpx3) and Ucp2 was measured by quantitative RT-PCR in the placental labyrinth zone (LZ) and junctional zone (JZ). Cytosolic superoxide dismutase (SOD), mitochondrial SOD and catalase (CAT) activities were also analyzed. Maternal n-3 PUFA supplementation increased LZ mRNA expression of Cat at both gestational days (2- and 1.5-fold respectively; P&amp;lt;0.01) and female Sod2 at day 22 (1.4-fold, P&amp;lt;0.01). Cytosolic SOD activity increased with n-3 PUFA supplementation at day 22 (1.3-fold, P&amp;lt;0.05). Sod1 and Txn1 expression decreased marginally (30 and 22%, P&amp;lt;0.05). JZ antioxidant defences were largely unaffected by diet. Despite increased LZ antioxidant defences, maternal n-3 PUFA supplementation did not protect against placental IR-induced growth restriction of the fetus and placental LZ.<\/jats:p>","DOI":"10.1530\/rep-13-0282","type":"journal-article","created":{"date-parts":[[2013,9,11]],"date-time":"2013-09-11T07:13:02Z","timestamp":1378883582000},"page":"539-547","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":16,"title":["Maternal omega-3 fatty acid intake increases placental labyrinthine antioxidant capacity but does not protect against fetal growth restriction induced by placental ischaemia\u2013reperfusion injury"],"prefix":"10.1093","volume":"146","author":[{"given":"Megan L","family":"Jones","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter J","family":"Mark","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brendan J","family":"Waddell","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2013,12,1]]},"reference":[{"key":"2026010712274697100_bib1","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1007\/s00383-012-3144-0","article-title":"Effect of an omega-3 lipid emulsion in reducing oxidative stress in a rat model of intestinal ischemia\u2013reperfusion injury","volume":"28","author":"Arisue","year":"2012","journal-title":"Pediatric Surgery International"},{"key":"2026010712274697100_bib2","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1038\/82565","article-title":"Disruption of the uncoupling protein-2 gene in mice reveals a role in immunity and reactive oxygen species production","volume":"26","author":"Arsenijevic","year":"2000","journal-title":"Nature Genetics"},{"key":"2026010712274697100_bib3","first-page":"275","article-title":"Oral omega-3 fatty acid for reduction of kidney dysfunction induced by reperfusion injury in rats","volume":"6","author":"Ashtiyani","year":"2012","journal-title":"Iranian Journal of Kidney Diseases"},{"key":"2026010712274697100_bib4","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1089\/ars.2011.4214","article-title":"Is there a role for isofurans and neuroprostanes in pre-eclampsia and normal pregnancy?","volume":"16","author":"Barden","year":"2012","journal-title":"Antioxidants & Redox Signaling"},{"key":"2026010712274697100_bib5","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.placenta.2005.01.002","article-title":"Oxidant status in maternal and cord plasma and placental tissue in gestational diabetes","volume":"27","author":"Biri","year":"2006","journal-title":"Placenta"},{"key":"2026010712274697100_bib6","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1159\/000106488","article-title":"Role of oxidative stress in intrauterine growth restriction","volume":"64","author":"Biri","year":"2007","journal-title":"Gynecologic and Obstetric Investigation"},{"key":"2026010712274697100_bib7","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/j.jnutbio.2012.02.014","article-title":"Protective effects of dietary EPA and DHA on ischemia\u2013reperfusion-induced intestinal stress","volume":"24","author":"Brahmbhatt","year":"2013","journal-title":"Journal of Nutritional Biochemistry"},{"key":"2026010712274697100_bib8","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.bpobgyn.2010.10.016","article-title":"Oxidative stress","volume":"25","author":"Burton","year":"2011","journal-title":"Best Practice & Research. 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