{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:17:12Z","timestamp":1790738232622,"version":"4.1.0"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Kisspeptin acts as an upstream regulator of the hypothalamus\u2013pituitary\u2013gonad axis, which is one of the main regulatory systems for mammalian reproduction. Kiss1 and its receptor Kiss1r (also known as G protein-coupled receptor 54 (Gpr54)) are expressed in various organs, but their functions are not well understood. The purpose of this study was to investigate the expression profiles and functions of kisspeptin and KISS1R in the reproductive tissues of imprinting control region mice. To identify the expression pattern and location of kisspeptin and KISS1R in gonads, testes and ovarian tissues were examined by immunohistochemical or immunofluorescent staining. Kisspeptin and KISS1R were expressed primarily in Leydig cells and seminiferous tubules respectively. KISS1R was specifically localized in the acrosomal region of spermatids and mature spermatozoa. Kisspeptin, but not KISS1R, was expressed in the cumulus\u2013oocyte complex and oviductal epithelium of ovarian and oviductal tissues. The sperm intracellular calcium concentrations significantly increased in response to treatment with kisspeptin 10 in Fluo-4-loaded sperm. The IVF rates decreased after treatment of sperm with the kisspeptin antagonist peptide 234. These results suggest that kisspeptin and KISS1R might be involved in the fertilization process in the female reproductive tract. In summary, this study indicates that kisspeptin and KISS1R are expressed in female and male gametes, respectively, and in mouse reproductive tissues. These data strongly suggest that the kisspeptin system could regulate mammalian fertilization and reproduction.<\/jats:p>","DOI":"10.1530\/rep-13-0368","type":"journal-article","created":{"date-parts":[[2014,2,25]],"date-time":"2014-02-25T01:11:16Z","timestamp":1393290676000},"page":"835-845","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":69,"title":["Kisspeptin modulates fertilization capacity of mouse spermatozoa"],"prefix":"10.1093","volume":"147","author":[{"given":"Meng-Chieh","family":"Hsu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jyun-Yuan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue-Jia","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De-Shien","family":"Jong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuan-Hao","family":"Tsui","sequence":"additional","affiliation":[{"name":"Department of Animal Science and Technology \u00a0 College of Bio-Resources and Agriculture, National Taiwan University, No. 50, Ln. 155, Sec. 3, Keelung Road, Taipei 10673, Taiwan 1 \u00a0 Kaohsiung Veterans General Hospital, Kaohsiung 81346, Taiwan, Republic of China"},{"name":"Department of Obstetrics and Gynecology \u00a0 College of Bio-Resources and Agriculture, National Taiwan University, No. 50, Ln. 155, Sec. 3, Keelung Road, Taipei 10673, Taiwan 1 \u00a0 Kaohsiung Veterans General Hospital, Kaohsiung 81346, Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih-Hsien","family":"Chiu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2014,6,1]]},"reference":[{"key":"2026010712282235600_bib1","doi-asserted-by":"crossref","first-page":"4852","DOI":"10.1210\/en.2006-0117","article-title":"Expression of KiSS-1 in rat ovary: putative local regulator of ovulation?","volume":"147","author":"Castellano","year":"2006","journal-title":"Endocrinology"},{"key":"2026010712282235600_bib2","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1007\/400_2007_050","article-title":"GPR54 and kisspeptins","volume":"46","author":"Colledge","year":"2008","journal-title":"Results and Problems in Cell Differentiation"},{"key":"2026010712282235600_bib3","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1530\/REP-09-0134","article-title":"Ca2+-stores in sperm: their identities and functions","volume":"138","author":"Costello","year":"2009","journal-title":"Reproduction"},{"key":"2026010712282235600_bib4","first-page":"1729","article-title":"Kisspeptin is released from human prostate cancer cell lines but plasma kisspeptin is not elevated in patients with prostate cancer","volume":"23","author":"Curtis","year":"2010","journal-title":"Oncology Reports"},{"key":"2026010712282235600_bib5","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1002\/mrd.21124","article-title":"The \u201cswitching on\u201d of mammalian spermatozoa: molecular events involved in promotion and regulation of capacitation","volume":"77","author":"Fraser","year":"2010","journal-title":"Molecular Reproduction and Development"},{"key":"2026010712282235600_bib6","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1083\/jcb.200210057","article-title":"Phospholipase C\u03b44 is required for Ca2+ mobilization essential for acrosome reaction in sperm","volume":"161","author":"Fukami","year":"2003","journal-title":"Journal of Cell Biology"},{"key":"2026010712282235600_bib7","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1016\/j.bbrc.2003.11.066","article-title":"The KiSS-1 receptor GPR54 is essential for the development of the murine reproductive system","volume":"312","author":"Funes","year":"2003","journal-title":"Biochemical and Biophysical Research Communications"},{"key":"2026010712282235600_bib8","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1007\/s00441-007-0426-2","article-title":"Expression of KiSS-1 in rat oviduct: possible involvement in prevention of ectopic implantation?","volume":"329","author":"Gaytan","year":"2007","journal-title":"Cell and Tissue Research"},{"key":"2026010712282235600_bib9","doi-asserted-by":"crossref","first-page":"E520","DOI":"10.1152\/ajpendo.90895.2008","article-title":"KiSS-1 in the mammalian ovary: distribution of kisspeptin in human and marmoset and alterations in KiSS-1 mRNA levels in a rat model of ovulatory dysfunction","volume":"296","author":"Gaytan","year":"2009","journal-title":"American Journal of Physiology. 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