{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T18:26:50Z","timestamp":1767810410394,"version":"3.49.0"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2014,7,1]],"date-time":"2014-07-01T00:00:00Z","timestamp":1404172800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2014,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Scrapie in sheep is spread laterally by placental transmission of an infectious misfolded form (PrPSc) of a normal prion protein (PrPC) used as a template in PrPSc formation. We hypothesized that PrPC would be expressed in uterine and placental tissues and estradiol-17\u03b2 (E2) would affect uterine PrPC expression. PrPC expression was evaluated in the uterus of long-term ovariectomized (OVX) ewes treated with an E2 implant for 2\u201324\u200ah and in uteroplacental tissues from day 20 to day 30 of pregnancy. Expression of PrPC mRNA and PrPC protein increased in the uterus after E2 treatment of OVX ewes. In the maternal placenta, expression of PrPC mRNA and PrPC protein were unchanged, but in the fetal membranes (FM) PrPC mRNA and PrPC protein expression increased from day 20 to day 28. In the nonpregnant uterus, PrPC protein was immunolocalized at apical borders of the surface epithelium, in outer smooth muscle layers of large blood vessels, and in scattered stromal cells of the deep intercaruncular areas of the uterus. In the maternal placenta, PrPC protein was immunolocalized in the cytoplasm of flattened luminal epithelial cells apposed to the FM, whereas in the FM PrPC protein was in trophoblast cells and was also in several tissues of the developing embryo during early pregnancy. These data linking estrogen stimulation to increases in PrPC expression in uteroplacental tissues suggest that PrPC has a specific function during the estrous cycle and early pregnancy. Future studies should determine whether or not estrogen influences PrPC expression in other tissues, such as the nervous system and brain.<\/jats:p>","DOI":"10.1530\/rep-13-0548","type":"journal-article","created":{"date-parts":[[2014,3,25]],"date-time":"2014-03-25T01:28:37Z","timestamp":1395710917000},"page":"1-10","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":9,"title":["Prion (PrPC) expression in ovine uteroplacental tissues increases after estrogen treatment of ovariectomized ewes and during early pregnancy"],"prefix":"10.1093","volume":"148","author":[{"given":"Mary Lynn","family":"Johnson","sequence":"first","affiliation":[{"name":"Department of Animal Sciences"},{"name":"Center for Nutrition and Pregnancy \u00a0 North Dakota State University, Fargo, North Dakota 58108, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna T","family":"Grazul-Bilska","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences"},{"name":"Center for Nutrition and Pregnancy \u00a0 North Dakota State University, Fargo, North Dakota 58108, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lawrence P","family":"Reynolds","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences"},{"name":"Center for Nutrition and Pregnancy \u00a0 North Dakota State University, Fargo, North Dakota 58108, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dale A","family":"Redmer","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences"},{"name":"Center for Nutrition and Pregnancy \u00a0 North Dakota State University, Fargo, North Dakota 58108, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2014,7,1]]},"reference":[{"key":"2026010712292960700_bib1","doi-asserted-by":"crossref","first-page":"400","DOI":"10.1089\/ars.2012.4637","article-title":"Prion protein expression and functional importance in developmental angiogenesis: role in oxidative stress and copper homeostasis","volume":"18","author":"Alfaidy","year":"2013","journal-title":"Antioxidants & Redox Signaling"},{"key":"2026010712292960700_bib2","first-page":"976","article-title":"Sex hormones, aging, and Alzheimer's disease","volume":"4","author":"Barron","year":"2012","journal-title":"Frontiers in Bioscience"},{"key":"2026010712292960700_bib3","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1095\/biolreprod.106.054684","article-title":"Placental growth throughout the last two-thirds of pregnancy in sheep: vascular development and angiogenic factor expression","volume":"76","author":"Borowicz","year":"2007","journal-title":"Biology of Reproduction"},{"key":"2026010712292960700_bib4","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1038\/379339a0","article-title":"Normal host prion protein necessary for scrapie-induced neurotoxicity","volume":"379","author":"Brandner","year":"1996","journal-title":"Nature"},{"key":"2026010712292960700_bib5","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1038\/emboj.2013.6","article-title":"Post-translational changes to PrP alter transmissible spongiform encephalopathy strain properties","volume":"32","author":"Cancellotti","year":"2013","journal-title":"EMBO Journal"},{"key":"2026010712292960700_bib6","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1093\/toxsci\/kfq049","article-title":"Manganese upregulates cellular prion protein and contributes to altered stabilization and proteolysis: relevance to role of metals in pathogenesis of prion disease","volume":"115","author":"Choi","year":"2010","journal-title":"Toxicological Sciences"},{"key":"2026010712292960700_bib7","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1038\/nrmicro2650","article-title":"De novo generation of prion strains","volume":"11","author":"Colby","year":"2011","journal-title":"Nature Reviews. 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