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Our previous study has demonstrated that PGF2A can modulate uterine activation proteins (UAPs) in cultured pregnant human myometrial smooth muscle cells (HMSMCs). The objective of this study was to define the signalling pathways responsible for PGF2A modulation of UAPs in myometrium. It was found that PGF2A stimulated the expression of (GJA1) connexin 43 (CX43), prostaglandin endoperoxide synthase 2 (PTGS2) and oxytocin receptor (OTR) in cultured HMSMCs. The inhibitors of phospholipase C (PLC) and protein kinase C (PKC) blocked PGF2A-stimulated expression of CX43. The inhibitors of ERK, P38 and NF\u03baB also blocked the effect of PGF2A on CX43 expression, whereas PI3K and calcineurin\/nuclear factor of activated T-cells (NFAT) pathway inhibitors did not reverse the effect of PGF2A on CX43. For PTGS2 and OTR, PLC, PI3K, P38 and calcineurin\/NFAT signalling pathways were involved in PGF2A action, whereas PKC and NF\u03baB signalling were not involved. In addition, PGF2A activated NFAT, PI3K, NF\u03baB, ERK and P38 signalling pathways. Our data suggest that PGF2A stimulates CX43, PTGS2 and OTR through divergent signalling pathways.<\/jats:p>","DOI":"10.1530\/rep-14-0479","type":"journal-article","created":{"date-parts":[[2014,10,24]],"date-time":"2014-10-24T00:07:03Z","timestamp":1414109223000},"page":"139-146","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":40,"title":["Prostaglandin F2\u03b1 regulates the expression of uterine activation proteins via multiple signalling pathways"],"prefix":"10.1093","volume":"149","author":[{"given":"Chen","family":"Xu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xingji","family":"You","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weina","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianqian","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Physiology Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China 1 Changhai Hospital, Shanghai, China 2 599 Hongfeng Road, Shanghai 201206, China"},{"name":"Department of Obstetrics and Gynecology Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China 1 Changhai Hospital, Shanghai, China 2 599 Hongfeng Road, Shanghai 201206, China"},{"name":"Maternity and Child Health Hospital of Pudong New District Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China 1 Changhai Hospital, Shanghai, China 2 599 Hongfeng Road, Shanghai 201206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoying","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Physiology Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China 1 Changhai Hospital, Shanghai, China 2 599 Hongfeng Road, Shanghai 201206, China"},{"name":"Department of Obstetrics and Gynecology Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China 1 Changhai Hospital, Shanghai, China 2 599 Hongfeng Road, Shanghai 201206, China"},{"name":"Maternity and Child Health Hospital of Pudong New District Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China 1 Changhai Hospital, Shanghai, China 2 599 Hongfeng Road, Shanghai 201206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Physiology Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China 1 Changhai Hospital, Shanghai, China 2 599 Hongfeng Road, Shanghai 201206, China"},{"name":"Department of Obstetrics and Gynecology Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China 1 Changhai Hospital, Shanghai, China 2 599 Hongfeng Road, Shanghai 201206, China"},{"name":"Maternity and Child Health Hospital of Pudong New District Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, China 1 Changhai Hospital, Shanghai, China 2 599 Hongfeng Road, Shanghai 201206, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Ni","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2015,1,1]]},"reference":[{"key":"2026010712414862800_bib1","doi-asserted-by":"crossref","first-page":"e37750","DOI":"10.1371\/journal.pone.0037750","article-title":"Angiotensin II activates the calcineurin\/NFAT signaling pathway and induces cyclooxygenase-2 expression in rat endometrial stromal cells","volume":"7","author":"Abraham","year":"2012","journal-title":"PLoS ONE"},{"key":"2026010712414862800_bib2","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1095\/biolreprod59.5.1069","article-title":"Prostaglandin F2\u03b1 treatment in vivo, but not in vitro, stimulates protein kinase C-activated superoxide production by nonsteroidogenic cells of the rat corpus luteum","volume":"59","author":"Aten","year":"1998","journal-title":"Biology of Reproduction"},{"key":"2026010712414862800_bib3","doi-asserted-by":"crossref","first-page":"130","DOI":"10.1016\/S0169-328X(97)00287-8","article-title":"NGF, cyclic AMP, and phorbol esters regulate oxytocin receptor gene transcription in SK-N-SH and MCF7 cells","volume":"53","author":"Bale","year":"1998","journal-title":"Brain Research. 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