{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T14:08:22Z","timestamp":1790690902390,"version":"4.1.0"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2015,6,1]],"date-time":"2015-06-01T00:00:00Z","timestamp":1433116800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2015,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Administration of human FSH (hFSH) during the diestrus phase in cyclic rats is followed by a reduction in the preovulatory LH surge. This inhibitory action of FSH involves a decrease in the stimulatory effect of gonadotrope progesterone receptor (PR) activation, in a ligand-dependent (progesterone) and -independent (GNRH) manner. PR activation and action are mandatory for LH surge, and are dependent on the phosphorylation of serine (Ser) residues. Together with this post-translational modification, PR is marked for downregulation by proteasome machinery. These experiments used the western blotting technique to measure pituitary expression of PR-A and PR-B isoforms and phosphorylation levels of Ser294 and Ser400 PR-B in rats bearing i) hFSH treatment or ii) PR downregulation. Treatment with hFSH reduced LH secretion and increased that of estradiol in proestrus afternoon. hFSH injections, without altering PR-A and PR-B content or ratio, caused a reduction in phosphorylation of Ser294 and Ser400 but only when pituitaries were previously challenged with progesterone or GNRH for 2\u200ah. However, while pSer294 levels increased after 2\u200ah of pituitary incubation with progesterone or GNRH, those of pSer400 were not modified by these in vitro treatments. Finally, progesterone had a biphasic effect: in 2-h incubations increased pituitary PR-A and PR-B content, but after 8\u200ah caused downregulation and altered PR-A:PR-B ratio. The results provide a potential mechanism through which LH levels are decreased by hFSH administration and better understanding of the control of PR expression and phosphorylation in rat pituitaries.<\/jats:p>","DOI":"10.1530\/rep-14-0592","type":"journal-article","created":{"date-parts":[[2015,4,29]],"date-time":"2015-04-29T21:33:20Z","timestamp":1430343200000},"page":"615-623","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":17,"title":["Understanding the regulation of pituitary progesterone receptor expression and phosphorylation"],"prefix":"10.1093","volume":"149","author":[{"given":"Ana","family":"Gordon","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 C","family":"Garrido-Gracia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafaela","family":"Aguilar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 E","family":"S\u00e1nchez-Criado","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2015,6,1]]},"reference":[{"key":"2026010712413116800_bib1","doi-asserted-by":"crossref","first-page":"988","DOI":"10.1111\/j.1365-2826.2007.01613.x","article-title":"Facilitation or inhibition of the oestradiol-induced gonadotrophin surge in the immature female rat by progesterone: effects on pituitary responsiveness to gonadotrophin-releasing hormone (GNRH), GNRH self-priming and pituitary mRNAs for the progesterone receptor A and B isoforms","volume":"19","author":"Attardi","year":"2007","journal-title":"Journal of Neuroendocrinology"},{"key":"2026010712413116800_bib2","doi-asserted-by":"crossref","first-page":"10457","DOI":"10.1074\/jbc.272.16.10457","article-title":"Differential phosphorylation of chicken progesterone receptor in hormone-dependent and ligand-independent activation","volume":"272","author":"Bai","year":"1997","journal-title":"Journal of Biological Chemistry"},{"key":"2026010712413116800_bib3","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1677\/joe.0.1630079","article-title":"Antiprogestins RU486 and ZK299 suppress basal and LHRH-stimulated FSH and LH secretion at pituitary level in the rat in an oestrous cycle stage-dependent manner","volume":"163","author":"Bellido","year":"1999","journal-title":"Journal of Endocrinology"},{"key":"2026010712413116800_bib4","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1530\/jrf.0.0820329","article-title":"Effects of ovarian hyperstimulation and isolated preovulatory follicles on LH responses to GnRH in rats","volume":"82","author":"Busbridge","year":"1988","journal-title":"Journal of Reproduction and Fertility"},{"key":"2026010712413116800_bib5","doi-asserted-by":"crossref","first-page":"3993","DOI":"10.1097\/00001756-199812210-00001","article-title":"Progesterone receptor isoforms are differentially regulated by sex steroids in the rat forebrain","volume":"9","author":"Camacho-Arroyo","year":"1998","journal-title":"Neuroreport"},{"key":"2026010712413116800_bib6","doi-asserted-by":"crossref","first-page":"1477","DOI":"10.1210\/endo.141.4.7428","article-title":"Stimulation of gonadotropin-releasing hormone surges by estrogen. 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