{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T13:55:28Z","timestamp":1788357328124,"version":"build-2803163510"},"reference-count":75,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Depending on the stage of gonad maturation, as well as other factors, gonadal steroids can exert either a positive or negative feedback at the brain and pituitary level. While this has been demonstrated in many teleost species, little is known about the nature of steroid feedback in Gadiform fish. Using an optimized in vitro model system of the Atlantic cod pituitary, the present study investigated the potential effects of two physiologically relevant doses of estradiol, testosterone (TS) or dihydrotestosterone (DHTS) on cell viability and gene expression of gonadotropin subunits (fshb\/lhb) and two suggested reproduction-relevant gonadotropin-releasing hormone receptors (gnrhr1b\/gnrhr2a) during three stages of sexual maturity. In general, all steroids stimulated cell viability in terms of metabolic activity and membrane integrity. Furthermore, all steroids affected fshb expression, with the effect depending on both the specific steroid, dose and maturity status. Conversely, only DHTS exposure affected lhb levels, and this occurred only during the spawning season. Using single-cell qPCR, co-transcription of gnrhr1b and gnrhr2a was confirmed to both fshb- and lhb- expressing gonadotropes, with gnrhr2a being the most prominently expressed isoform. While steroid exposure had no effect on gnrhr1b expression, all steroids affected gnrhr2a transcript levels in at least one maturity stage. These and previous results from our group point to Gnrhr2a as the main modulator of gonadotropin regulation in cod and that regulation of its gene expression level might function as a direct mechanism for steroid feedback at the pituitary level.<\/jats:p>","DOI":"10.1530\/rep-17-0208","type":"journal-article","created":{"date-parts":[[2017,8,5]],"date-time":"2017-08-05T20:10:18Z","timestamp":1501963818000},"page":"581-594","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":23,"title":["Sex steroids differentially regulate\n                    <i>fshb<\/i>\n                    ,\n                    <i>lhb<\/i>\n                    and\n                    <i>gnrhr<\/i>\n                    expression in Atlantic cod (\n                    <i>Gadus morhua<\/i>\n                    )"],"prefix":"10.1093","volume":"154","author":[{"given":"Kristine","family":"von Krogh","sequence":"first","affiliation":[{"name":"Department of Basic Sciences and Aquatic Medicine , Norwegian University of Life Sciences, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunnveig Toft","family":"Bj\u00f8rndal","sequence":"additional","affiliation":[{"name":"Department of Biosciences , University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rasoul","family":"Nourizadeh-Lillabadi","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences and Aquatic Medicine , Norwegian University of Life Sciences, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kjetil","family":"Hodne","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences and Aquatic Medicine , Norwegian University of Life Sciences, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Ropstad","sequence":"additional","affiliation":[{"name":"Department of Production Animal Clinical Sciences , Norwegian University of Life Science, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trude M","family":"Haug","sequence":"additional","affiliation":[{"name":"Department of Biosciences , University of Oslo, Oslo, Norway"},{"name":"Department of Oral Biology , University of Oslo, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Finn-Arne","family":"Weltzien","sequence":"additional","affiliation":[{"name":"Department of Basic Sciences and Aquatic Medicine , Norwegian University of Life Sciences, Oslo, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2017,11,1]]},"reference":[{"key":"2026010712153120000_bib1","doi-asserted-by":"crossref","first-page":"631","DOI":"10.1095\/biolreprod.108.071340","article-title":"Photoperiod-modulated testis maturation in Atlantic cod (Gadus morhua, L.)","volume":"80","author":"Almeida","year":"2009","journal-title":"Biology of 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