{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T18:11:58Z","timestamp":1767809518763,"version":"3.49.0"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2017,12,1]],"date-time":"2017-12-01T00:00:00Z","timestamp":1512086400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2017,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Lipid metabolism disorders (dyslipidemia) are causes of male infertility, but little is known about their impact on male gametes when considering post-testicular maturation events, given that studies concentrate most often on endocrine dysfunctions and testicular consequences. In this study, three-month-old wild-type (wt) and Liver-X-Receptors knock out (Lxr\u03b1;\u03b2\u2212\/\u2212) males were fed four weeks with a control or a lipid-enriched diet containing 1.25% cholesterol (high cholesterol diet (HCD)). The HCD triggered a dyslipidemia leading to sperm post-testicular alterations and infertility. Sperm lipids were analyzed by LC\u2013MS and those from Lxr\u03b1;\u03b2\u2212\/\u2212 males fed the HCD showed higher chol\/PL and PC\/PE ratios compared to wt-HCD (P\u2009&amp;lt;\u20090.05) and lower oxysterol contents compared to wt (P\u2009&amp;lt;\u20090.05) or Lxr\u03b1;\u03b2\u2212\/\u2212 (P\u2009&amp;lt;\u20090.05). These modifications impaired membrane-associated events triggering the tyrosine phosphorylation normally occurring during the capacitation process, as shown by phosphotyrosine Western blots. Using flow cytometry, we showed that a smaller subpopulation of spermatozoa from Lxr\u03b1;\u03b2\u2212\/\u2212-HCD males could raise their membrane fluidity during capacitation (P\u2009&amp;lt;\u20090.05 vs wt or wt-HCD) as well as their intracellular calcium concentration (P\u2009&amp;lt;\u20090.05 vs Lxr\u03b1;\u03b2\u2212\/\u2212 and P\u2009&amp;lt;\u20090.001 vs wt). The accumulation of the major sperm calcium efflux pump (PMCA4) was decreased in Lxr\u03b1;\u03b2\u2212\/\u2212 males fed the HCD (P\u2009&amp;lt;\u20090.05 vs Lxr\u03b1;\u03b2\u2212\/\u2212 and P\u2009&amp;lt;\u20090.001 vs wt). This study is the first showing an impact of dyslipidemia on post-testicular sperm maturation with consequences on the capacitation signaling cascade. It may lead to the identification of fertility prognostic markers in this pathophysiological situation, which could help clinicians to better understand male infertilities which are thus far classified as idiopathic.<\/jats:p>","DOI":"10.1530\/rep-17-0467","type":"journal-article","created":{"date-parts":[[2017,10,2]],"date-time":"2017-10-02T20:20:19Z","timestamp":1506975619000},"page":"827-842","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":15,"title":["Dyslipidemia alters sperm maturation and capacitation in LXR-null mice"],"prefix":"10.1093","volume":"154","author":[{"given":"M","family":"Whitfield","sequence":"first","affiliation":[{"name":"Team MEPTI (M\u00e9canismes Post-Testiculaires de l\u2019Infertilit\u00e9) , Universit\u00e9 Clermont Auvergne, UMR GReD (G\u00e9n\u00e9tique Reproduction & D\u00e9veloppement), CNRS 6293, INSERM U1103, Clermont-Ferrand, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Guiton","sequence":"additional","affiliation":[{"name":"Team MEPTI (M\u00e9canismes Post-Testiculaires de l\u2019Infertilit\u00e9) , Universit\u00e9 Clermont Auvergne, UMR GReD (G\u00e9n\u00e9tique Reproduction & D\u00e9veloppement), CNRS 6293, INSERM U1103, Clermont-Ferrand, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Rispal","sequence":"additional","affiliation":[{"name":"Team MEPTI (M\u00e9canismes Post-Testiculaires de l\u2019Infertilit\u00e9) , Universit\u00e9 Clermont Auvergne, UMR GReD (G\u00e9n\u00e9tique Reproduction & D\u00e9veloppement), CNRS 6293, INSERM U1103, Clermont-Ferrand, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Acar","sequence":"additional","affiliation":[{"name":"Centre des Sciences du Go\u00fbt et de l\u2019Alimentation , AgroSup Dijon, CNRS, INRA, Universit\u00e9 Bourgogne Franche-Comt\u00e9, F-21000 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Kocer","sequence":"additional","affiliation":[{"name":"Team MEPTI (M\u00e9canismes Post-Testiculaires de l\u2019Infertilit\u00e9) , Universit\u00e9 Clermont Auvergne, UMR GReD (G\u00e9n\u00e9tique Reproduction & D\u00e9veloppement), CNRS 6293, INSERM U1103, Clermont-Ferrand, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J R","family":"Drevet","sequence":"additional","affiliation":[{"name":"Team MEPTI (M\u00e9canismes Post-Testiculaires de l\u2019Infertilit\u00e9) , Universit\u00e9 Clermont Auvergne, UMR GReD (G\u00e9n\u00e9tique Reproduction & D\u00e9veloppement), CNRS 6293, INSERM U1103, Clermont-Ferrand, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Saez","sequence":"additional","affiliation":[{"name":"Team MEPTI (M\u00e9canismes Post-Testiculaires de l\u2019Infertilit\u00e9) , Universit\u00e9 Clermont Auvergne, UMR GReD 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