{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T01:47:14Z","timestamp":1790387234894,"version":"4.1.0"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"vor","delay-in-days":2254,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The uterine microenvironment during pre-implantation presents a pro-survival milieu and is essential for embryo elongation in ruminants. The European roe deer (Careolus capreolus) pre-implantation embryo development is characterised by a 4-month period of reduced development, embryonic diapause, after which the embryo rapidly elongates and implants. We investigated the uterine fluid proteome by label-free liquid chromatography tandem mass spectrometry at four defined stages covering the phase of reduced developmental pace (early diapause, mid-diapause and late diapause) and embryo elongation. We hypothesised that embryo development during diapause is halted by the lack of signals that support progression past the blastocyst stage. Three clusters of differentially abundant proteins were identified by a self-organising tree algorithm: (1) gradual reduction over development; (2) stable abundance during diapause, followed by a sharp rise at elongation; and (3) gradual increase over development. Proteins in the different clusters were subjected to gene ontology analysis. \u2018Cellular detoxification\u2019 in cluster 1 was represented by alcohol dehydrogenase, glutathione S-transferase and peroxiredoxin-2. ATP-citrate synthase, nucleolin, lamin A\/C, and purine phosphorylase as cell proliferation regulators were found in cluster 2 and \u2018cortical cytoskeleton\u2019, \u2018regulation of substrate adhesion-dependent cell spreading\u2019 and \u2018melanosome\u2019 were present in cluster 3. Cell cycle promoters were higher abundant at elongation than during diapause, and polyamines presence indicates their role in diapause regulation. This study provides a comprehensive overview of proteins in the roe deer uterine fluid during diapause and forms a basis for studies aiming at understanding the impact of the lack of cell cycle promoters during diapause.<\/jats:p>","DOI":"10.1530\/rep-19-0022","type":"journal-article","created":{"date-parts":[[2019,4,2]],"date-time":"2019-04-02T07:32:02Z","timestamp":1554190322000},"page":"13-24","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":18,"title":["Uterine fluid proteome changes during diapause and resumption of embryo development in roe deer (\n                    <i>Capreolus capreolus<\/i>\n                    )"],"prefix":"10.1093","volume":"158","author":[{"given":"V A","family":"van der Weijden","sequence":"first","affiliation":[{"name":"ETH Zurich , Animal Physiology, Institute of Agricultural Sciences, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J T","family":"Bick","sequence":"additional","affiliation":[{"name":"ETH Zurich , Animal Physiology, Institute of Agricultural Sciences, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Bauersachs","sequence":"additional","affiliation":[{"name":"Genetics and Functional Genomics , Clinic of Reproductive Medicine, Department for Farm Animals, University of Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G J","family":"Arnold","sequence":"additional","affiliation":[{"name":"Laboratory for Functional Genome Analysis LAFUGA , Ludwig-Maximilians-Universit\u00e4t Munich, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Fr\u00f6hlich","sequence":"additional","affiliation":[{"name":"Laboratory for Functional Genome Analysis LAFUGA , Ludwig-Maximilians-Universit\u00e4t Munich, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B","family":"Drews","sequence":"additional","affiliation":[{"name":"ETH Zurich , Animal Physiology, Institute of Agricultural Sciences, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S E","family":"Ulbrich","sequence":"additional","affiliation":[{"name":"ETH Zurich , Animal Physiology, Institute of Agricultural Sciences, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,7,1]]},"reference":[{"key":"2026010712202620700_bib1","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1530\/jrf.0.0400333","article-title":"Calcium and zinc in the endometrium and uterine flushings of the roe deer (Capreolus capreolus) during delayed implantation","volume":"40","author":"Aitken","year":"1974","journal-title":"Journal of Reproduction and Fertility"},{"key":"2026010712202620700_bib2","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1530\/jrf.0.0390225","article-title":"Delayed implantation in roe deer (Capreolus capreolus)","volume":"39","author":"Aitken","year":"1974","journal-title":"Journal of Reproduction and Fertility"},{"key":"2026010712202620700_bib3","doi-asserted-by":"publisher","first-page":"439","DOI":"10.1530\/jrf.0.0460439","article-title":"Uterine secretion of fructose in the roe deer","volume":"46","author":"Aitken","year":"1976","journal-title":"Journal of Reproduction and Fertility"},{"key":"2026010712202620700_bib4","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1530\/jrf.0.0340481","article-title":"Histological and ultrastructural changes in the blastocyst and reproductive tract of the roe deer, Capreolus capreolus, during delayed implantation","volume":"34","author":"Aitken","year":"1973","journal-title":"Journal of Reproduction and Fertility"},{"key":"2026010712202620700_bib5","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1016\/S0022-2836(05)80360-2","article-title":"Basic local alignment search tool","volume":"215","author":"Altschul","year":"1990","journal-title":"Journal of Molecular Biology"},{"key":"2026010712202620700_bib6","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene ontology: tool for the unification of biology. 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