{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T18:09:43Z","timestamp":1778350183073,"version":"3.51.4"},"reference-count":66,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2019,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Avian uterine fluid (UF) and uterovaginal sperm storage tubules (SST) are key components in accepting sperm in SST, maintaining sperm function for several weeks, releasing sperm from SST and their ascent through the uterus. To improve the understanding of sperm storage processes requires investigating UF and SST. This study aimed to identify proteins modulated by sperm in the hen\u2019s genital tract and to highlight their role during sperm storage. Two genetic lines of hens exhibiting long (F+) or short (F\u2212) sperm storage ability were used. GeLC MS\/MS analysis was used to establish a quantitative inventory of proteins regulated after insemination in both lines. The proteomic data are available via ProteomeXchange with identifier PXD013514. Immunohistochemistry was used to identify high (ANXA4\/ANXA5\/OCX32) and low (HSPA8\/PIGR) fertility markers in the uterovaginal junction. Our results demonstrated that sperm induced a significant and rapid change in the UF proteomic content and also in the SST epithelium. In F+ hens, mobilization of the ANXA4 protein in the apical part of SST cells after insemination was associated with increased levels of some proteoglycans and binding proteins, and also antimicrobial eggshell matrix protein (OCX32) in the UF. We also observed increased levels of lipid transporters involved in egg formation (VTG1-2, APOA1-4-H). In F\u2212 hens, insemination induced increased levels of PIGR in both UF and SST, of ANXA5 in SST, of UF enzymes exhibiting metallopeptidase activity and mucins. In conclusion, sperm induced significant changes in the UF proteomic content. This study also provides evidence that the SST immune system plays a major role in regulating sperm storage.<\/jats:p>","DOI":"10.1530\/rep-19-0079","type":"journal-article","created":{"date-parts":[[2019,8,22]],"date-time":"2019-08-22T09:16:07Z","timestamp":1566465367000},"page":"335-356","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":8,"title":["Proteomic analysis of uterine fluid of fertile and subfertile hens before and after insemination"],"prefix":"10.1093","volume":"158","author":[{"given":"Cindy","family":"Riou","sequence":"first","affiliation":[{"name":"PRC , INRA, CNRS, IFCE, Universit\u00e9 de Tours, Nouzilly, France"},{"name":"ALLICE , Station de Ph\u00e9notypage, Lieu-Dit Le Perroi, Nouzilly, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aur\u00e9lien","family":"Brionne","sequence":"additional","affiliation":[{"name":"BOA , INRA, Universit\u00e9 de Tours, Nouzilly, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz","family":"Cordeiro","sequence":"additional","affiliation":[{"name":"PRC , INRA, CNRS, IFCE, Universit\u00e9 de Tours, Nouzilly, France"},{"name":"Federal University of Semi Arid Region , Mossoro, Rio Grande do Norte, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gr\u00e9goire","family":"Harichaux","sequence":"additional","affiliation":[{"name":"INRA , Universit\u00e9 de Tours, CHU de Tours, Plate-forme de Chirurgie et Imagerie pour la Recherche et l\u2019Enseignement (CIRE), P\u00f4le d\u2019Analyse et d\u2019Imagerie des Biomol\u00e9cules (PAIB), Nouzilly, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Audrey","family":"Gargaros","sequence":"additional","affiliation":[{"name":"INRA , Universit\u00e9 de Tours, CHU de Tours, Plate-forme de Chirurgie et Imagerie pour la Recherche et l\u2019Enseignement (CIRE), P\u00f4le d\u2019Analyse et d\u2019Imagerie des 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