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In the current follow-up study, we examined whether ER stress attenuation during in vitro maturation influences meiotic maturation, oocyte quality, and subsequent embryonic development. Bovine cumulus oocyte complexes (COCs) derived from slaughterhouse ovaries were matured with or without tauroursodeoxycholic acid (TUDCA), a selective inhibitor of ER stress (0, 50, 100, and 200 \u00b5M) for 22 h followed by in vitro fertilization, and zygotes were cultured for 8 days. Of the different doses of TUDCA, 100 \u03bcM TUDCA significantly increased the maturation rate, and decreased reactive oxygen species in denuded oocytes, and appeared lower number of apoptotic cells in matured COCs. Subsequently, treatment of TUDCA (100 \u00b5M) decreased the localization and amount of GRP78\/BIP protein level as well as ER stress (GRP78\/BIP, PERK, IER1, ATF4, and XBP1) and apoptosis (CHOP and BAX)-related gene expression, while it increased the anti-apoptotic gene BCL2 level in matured COCs. Moreover, addition of TUDCA (100 \u00b5M) during IVM significantly improved the blastocyst formation rate (43.6\u2009\u00b1\u20091.8% vs 49.7\u2009\u00b1\u20091.3%) and decreased the number of apoptotic cells (7.7\u2009\u00b1\u20091.1% vs 5.03\u2009\u00b1\u20090.6%) in blastocysts. These findings suggest that the presence of ER stress during maturation impairs the developmental competence of bovine COCs and that this process can be reversed by TUDCA.<\/jats:p>","DOI":"10.1530\/rep-19-0492","type":"journal-article","created":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T11:51:44Z","timestamp":1580125904000},"page":"361-370","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":42,"title":["Endoplasmic reticulum stress attenuation promotes bovine oocyte maturation\n                    <i>in vitro<\/i>"],"prefix":"10.1093","volume":"159","author":[{"given":"Hafiza","family":"Khatun","sequence":"first","affiliation":[{"name":"Faculty of Agriculture , Saga University, Saga, Japan"},{"name":"Faculty of Animal Husbandry , Bangladesh Agricultural University, Mymensingh, Bangladesh"},{"name":"The United Graduate School of Agricultural Sciences , Kagoshima University, Kagoshima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiko","family":"Wada","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture , Saga University, Saga, Japan"},{"name":"The United Graduate School of Agricultural Sciences , Kagoshima University, Kagoshima, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiro","family":"Konno","sequence":"additional","affiliation":[{"name":"The United Graduate School of Agricultural Sciences , Kagoshima University, Kagoshima, Japan"},{"name":"Faculty of Agriculture , University of the Ryukyus, Okinawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideki","family":"Tatemoto","sequence":"additional","affiliation":[{"name":"The United Graduate School of Agricultural Sciences , Kagoshima University, Kagoshima, Japan"},{"name":"Faculty of Agriculture , University of the Ryukyus, Okinawa, 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