{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T23:46:19Z","timestamp":1785714379186,"version":"3.56.0"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"5","content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:p>\n                    During embryo implantation, endometrial angiogenesis is regulated by signals originating from the endometrium itself and the developing embryo. It has been suggested that hCG may play a pro-angiogenic role; therefore, we sought to understand its regulatory role in blood vessel formation in human endometrium using in vivo and in vitro models. In the in vivo model, we screened 16 angiogenesis-related transcripts in the endometrium upon intrauterine administration of hCG. Oocyte donors were recruited and during their controlled ovarian stimulation cycle received a single dose of hCG or vehicle on the day of oocyte pick up during a cycle of ovarian stimulation. One hour before obtaining an endometrial sample, women received an intrauterine administration of vehicle or hCG (500, 1500 and 5000 IU). Transcript and protein analysis showed that MMP3 and VEGFA increased, whereas TIMP1 decreased. The\n                    <jats:italic>in vitro<\/jats:italic>\n                    analysis studied the angiogenic potential of conditioned medium (CM) from primary cultures of human endometrial stromal cells (ESC) stimulated with hCG. Using a 2D and 3D\n                    <jats:italic>in vitro<\/jats:italic>\n                    angiogenesis assays, our results indicate that CM from ESC almost completely inhibits the capillary-like structure formation in endothelial cells, overriding the pro-angiogenic effect of hCG; and this inhibition due to secreted factors present in CM specifically reduced the migration potential of endothelial cells. In conclusion, the endometrial stromal milieu seems to modulate the direct pro-angiogenic effects of hCG on endothelial cells during embryo implantation.\n                  <\/jats:p>","DOI":"10.1530\/rep-19-0559","type":"journal-article","created":{"date-parts":[[2020,8,7]],"date-time":"2020-08-07T11:38:58Z","timestamp":1596800338000},"page":"673-684","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":2,"title":["Human endometrial stromal cells inhibit the pro-angiogenic stimulus of hCG in vitro"],"prefix":"10.1093","volume":"160","author":[{"given":"Marcia","family":"Riboldi","sequence":"first","affiliation":[{"name":"1Huntington Medicina Reprodutiva, S\u00e3o Paulo, Brasil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivonne","family":"Nazir","sequence":"additional","affiliation":[{"name":"2IDIMI, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bel\u00e9n","family":"Jara","sequence":"additional","affiliation":[{"name":"2IDIMI, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felipe","family":"Argando\u00f1a","sequence":"additional","affiliation":[{"name":"2IDIMI, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Valencia","sequence":"additional","affiliation":[{"name":"2IDIMI, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulo C","family":"Serafini","sequence":"additional","affiliation":[{"name":"1Huntington Medicina Reprodutiva, S\u00e3o Paulo, Brasil"},{"name":"3Disciplina de Ginecologia, Departamento de Obstetr\u00edcia e Ginecologia, Faculdade de Medicina, Universidade de S\u00e3o Paulo (FMUSP), S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo Leme Alves","family":"Motta","sequence":"additional","affiliation":[{"name":"1Huntington Medicina Reprodutiva, S\u00e3o Paulo, Brasil"},{"name":"4Disciplina de Ginecologia Endocrinol\u00f3gica, Departamento de Ginecologia, Escola Paulista de Medicina da Universidade Federal de S\u00e3o Paulo (UNIFSEP-EPM), S\u00e3o Paulo, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Denisse","family":"Mena-Silva","sequence":"additional","affiliation":[{"name":"2IDIMI, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinaldo","family":"Gonz\u00e1lez-Ramos","sequence":"additional","affiliation":[{"name":"2IDIMI, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M Cecilia","family":"Johnson","sequence":"additional","affiliation":[{"name":"2IDIMI, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariel","family":"Fuentes","sequence":"additional","affiliation":[{"name":"2IDIMI, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina","family":"Sequeira","sequence":"additional","affiliation":[{"name":"2IDIMI, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Tapia-Pizarro","sequence":"additional","affiliation":[{"name":"2IDIMI, Facultad de Medicina, Universidad de Chile, Santiago, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","container-title":["Reproduction"],"original-title":[],"link":[{"URL":"https:\/\/rep.bioscientifica.com\/view\/journals\/rep\/160\/5\/REP-19-0559.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/rep.bioscientifica.com\/downloadpdf\/journals\/rep\/160\/5\/REP-19-0559.xml","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T23:02:30Z","timestamp":1767567750000},"score":1,"resource":{"primary":{"URL":"https:\/\/rep.bioscientifica.com\/view\/journals\/rep\/160\/5\/REP-19-0559.xml"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,11]]},"references-count":0,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.1530\/rep-19-0559","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,11]]}}}