{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T16:30:20Z","timestamp":1781022620512,"version":"3.54.1"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2025,9,1]],"date-time":"2025-09-01T00:00:00Z","timestamp":1756684800000},"content-version":"vor","delay-in-days":1949,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Tubal endometriosis (tubal EM) is a subtype of endometriosis (EM) associated with fallopian tube impairments and infertility. Since the molecular mechanism underlying tubal EM is not clear, we assume that an aberrant transcriptome of fallopian tube epithelium and microenvironment changes caused by cytokines in tubal fluid are possible causes. The aim of this study was to identify potential hub mRNAs\/proteins of tubal EM through integrated transcriptomic and proteomic analyses and to elucidate significant pathways, cellular functions, and interaction networks during the initiation and progression of tubal EM. We obtained human fallopian tube epithelium and tubal fluid samples from patients with and without tubal EM. Tubal epithelia were analyzed using microarray, and tubal fluid was analyzed using quantitative label-free LC-MS\/MS. We identified differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) and determined common mRNAs\/protein. We observed 35 commonly deregulated mRNAs\/proteins, and IPA indicated that cellular movement, inflammatory response, and immune cell trafficking were significantly activated during the pathogenesis of tubal EM. We also identified acute phase response signaling pathway activation as a unique pathogenesis signature of tubal EM. Our results demonstrate that an integrated analysis of the transcriptome and proteome has the potential to reveal novel disease mechanisms at a molecular level.<\/jats:p>","DOI":"10.1530\/rep-19-0587","type":"journal-article","created":{"date-parts":[[2020,3,3]],"date-time":"2020-03-03T11:50:33Z","timestamp":1583236233000},"page":"601-614","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":11,"title":["Integrated analysis of mRNA and protein expression profiling in tubal endometriosis"],"prefix":"10.1093","volume":"159","author":[{"given":"Hang","family":"Qi","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology , International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China"},{"name":"Shanghai Key Laboratory Embryo Original Diseases , Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology , International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China"},{"name":"Shanghai Key Laboratory Embryo Original Diseases , Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoya","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology , International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China"},{"name":"Shanghai Key Laboratory Embryo Original Diseases , Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Qin","sequence":"additional","affiliation":[{"name":"School of Life Science and Technology , ShanghaiTech University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiling","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology , International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China"},{"name":"Shanghai Key Laboratory Embryo Original Diseases , Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqing","family":"He","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology , International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China"},{"name":"Shanghai Key Laboratory Embryo Original Diseases , Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology , International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China"},{"name":"Shanghai Key Laboratory Embryo Original Diseases , Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,5,1]]},"reference":[{"key":"2026010712223221800_bib1","doi-asserted-by":"publisher","first-page":"2088","DOI":"10.1021\/acs.jproteome.8b00970","article-title":"Comparative analysis of the transcriptome and proteome during mouse placental development","volume":"18","author":"Abdulghani","year":"2019","journal-title":"Journal of Proteome Research"},{"key":"2026010712223221800_bib2","doi-asserted-by":"publisher","first-page":"1420.e7","DOI":"10.1016\/j.fertnstert.2016.07.005","article-title":"Immune-inflammation gene signatures in endometriosis patients","volume":"106","author":"Ahn","year":"2016","journal-title":"Fertility and Sterility"},{"key":"2026010712223221800_bib3","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1083\/jcb.200409070","article-title":"TRPV4 channel is involved in the coupling of fluid viscosity changes to epithelial ciliary activity","volume":"168","author":"Andrade","year":"2005","journal-title":"Journal of Cell Biology"},{"key":"2026010712223221800_bib5","doi-asserted-by":"publisher","first-page":"103","DOI":"10.1210\/en.2017-00562","article-title":"Early endometriosis in females is directed by immune-mediated estrogen receptor \u03b1 and IL-6 cross-talk","volume":"159","author":"Burns","year":"2018","journal-title":"Endocrinology"},{"key":"2026010712223221800_bib6","doi-asserted-by":"publisher","first-page":"4560","DOI":"10.1096\/fj.201701382RR","article-title":"EGFR-mediated matrix metalloproteinase-7 up-regulation promotes epithelial-mesenchymal transition via ERK1-AP1 axis during ovarian endometriosis progression","volume":"32","author":"Chatterjee","year":"2018","journal-title":"FASEB Journal"},{"key":"2026010712223221800_bib7","doi-asserted-by":"publisher","first-page":"1476","DOI":"10.1016\/j.fertnstert.2013.07.1985","article-title":"A tumor necrosis factor-alpha inhibitor reduces the embryotoxic effects of endometriotic peritoneal fluid","volume":"100","author":"Chen","year":"2013","journal-title":"Fertility and Sterility"},{"key":"2026010712223221800_bib4","doi-asserted-by":"publisher","first-page":"2317","DOI":"10.3892\/etm.2017.4794","article-title":"Circulating and peritoneal fluid interleukin-6 levels and gene expression in pelvic endometriosis","volume":"14","author":"De Andrade","year":"2017","journal-title":"Experimental and Therapeutic Medicine"},{"key":"2026010712223221800_bib8","doi-asserted-by":"publisher","first-page":"803","DOI":"10.1530\/jrf.0.0830803","article-title":"The acute-phase response in endometriosis of women","volume":"83","author":"Dunselman","year":"1988","journal-title":"Journal of Reproduction and Fertility"},{"key":"2026010712223221800_bib9","doi-asserted-by":"publisher","first-page":"1261.e1","DOI":"10.1016\/j.fertnstert.2010.11.066","article-title":"Urinary peptide profiling identifies a panel of putative biomarkers for diagnosing and staging endometriosis","volume":"95","author":"El-Kasti","year":"2011","journal-title":"Fertility and Sterility"},{"key":"2026010712223221800_bib10","doi-asserted-by":"publisher","first-page":"2020","DOI":"10.1093\/humrep\/des127","article-title":"Combined mRNA microarray and proteomic analysis of eutopic endometrium of women with and without endometriosis","volume":"27","author":"Fassbender","year":"2012","journal-title":"Human Reproduction"},{"key":"2026010712223221800_bib11","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1159\/000452660","article-title":"Prevalence and symptomatic burden of diagnosed endometriosis in the United States: national estimates from a cross-sectional survey of 59,411 women","volume":"82","author":"Fuldeore","year":"2017","journal-title":"Gynecologic and Obstetric Investigation"},{"key":"2026010712223221800_bib12","doi-asserted-by":"publisher","first-page":"e12690","DOI":"10.1111\/aji.12690","article-title":"Hypoxia-inhibited DUSP2 expression promotes IL-6\/STAT3 signaling in endometriosis","volume":"78","author":"Hsiao","year":"2017","journal-title":"American Journal of Reproductive Immunology"},{"key":"2026010712223221800_bib13","doi-asserted-by":"publisher","first-page":"183","DOI":"10.3892\/mmr.2013.1469","article-title":"Identification of biomarkers for endometriosis in eutopic endometrial cells from patients with endometriosis using a proteomics approach","volume":"8","author":"Hwang","year":"2013","journal-title":"Molecular Medicine Reports"},{"key":"2026010712223221800_bib14","doi-asserted-by":"publisher","first-page":"2878","DOI":"10.1523\/JNEUROSCI.2534-16.2017","article-title":"Astrocytic orosomucoid-2 modulates microglial activation and neuroinflammation","volume":"37","author":"Jo","year":"2017","journal-title":"Journal of Neuroscience"},{"key":"2026010712223221800_bib15","doi-asserted-by":"publisher","first-page":"1497","DOI":"10.1016\/j.fertnstert.2004.05.089","article-title":"Association between human alpha 2-Heremans Schmidt glycoprotein (AHSG) polymorphism and endometriosis in Korean women","volume":"82","author":"Kim","year":"2004","journal-title":"Fertility and Sterility"},{"key":"2026010712223221800_bib16","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1093\/molehr\/gaw054","article-title":"TNFalpha-induced IKKbeta complex activation influences epithelial, but not stromal cell survival in endometriosis","volume":"22","author":"Kocbek","year":"2016","journal-title":"Molecular Human Reproduction"},{"key":"2026010712223221800_bib17","doi-asserted-by":"publisher","first-page":"327","DOI":"10.1016\/j.fertnstert.2018.10.013","article-title":"Pathogenesis of endometriosis: the genetic\/epigenetic theory","volume":"111","author":"Koninckx","year":"2019","journal-title":"Fertility and Sterility"},{"key":"2026010712223221800_bib18","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1016\/j.fertnstert.2005.12.024","article-title":"ProteinChip technology is a useful method in the pathogenesis and diagnosis of endometriosis: a preliminary study","volume":"86","author":"Kyama","year":"2006","journal-title":"Fertility and Sterility"},{"key":"2026010712223221800_bib19","doi-asserted-by":"publisher","first-page":"843","DOI":"10.1530\/jrf.0.0820843","article-title":"The formation and function of oviduct fluid","volume":"82","author":"Leese","year":"1988","journal-title":"Journal of Reproduction and Fertility"},{"key":"2026010712223221800_bib20","doi-asserted-by":"publisher","first-page":"R1","DOI":"10.1530\/JOE-16-0302","article-title":"Oviduct: roles in fertilization and early embryo development","volume":"232","author":"Li","year":"2017","journal-title":"Journal of Endocrinology"},{"key":"2026010712223221800_bib21","doi-asserted-by":"publisher","first-page":"144","DOI":"10.1095\/biolreprod.115.127969","article-title":"Oviduct-embryo interactions in cattle: two-way traffic or a one-way street?","volume":"92","author":"Maillo","year":"2015","journal-title":"Biology of Reproduction"},{"key":"2026010712223221800_bib22","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1093\/molehr\/gah097","article-title":"DNA microarray analysis of gene expression profiles in deep endometriosis using laser capture microdissection","volume":"10","author":"Matsuzaki","year":"2004","journal-title":"Molecular Human Reproduction"},{"key":"2026010712223221800_bib23","doi-asserted-by":"publisher","first-page":"2812","DOI":"10.1096\/fj.201600251R","article-title":"Transcriptomics and proteomics show that selenium affects inflammation, cytoskeleton, and cancer pathways in human rectal biopsies","volume":"30","author":"Meplan","year":"2016","journal-title":"FASEB Journal"},{"key":"2026010712223221800_bib24","doi-asserted-by":"publisher","first-page":"100074","DOI":"10.1016\/j.eurox.2019.100074","article-title":"Reassessment of prevalence of tubal endometriosis, and its associated clinicopathologic features and risk factors in premenopausal women received salpingectomy","volume":"4","author":"Qi","year":"2019","journal-title":"European Journal of Obstetrics and Gynecology and Reproductive Biology"},{"key":"2026010712223221800_bib25","doi-asserted-by":"publisher","first-page":"12436","DOI":"10.1038\/ncomms12436","article-title":"miR-625-3p regulates oxaliplatin resistance by targeting MAP2K6-p38 signalling in human colorectal adenocarcinoma cells","volume":"7","author":"Rasmussen","year":"2016","journal-title":"Nature Communications"},{"key":"2026010712223221800_bib26","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1148\/rg.312105090","article-title":"Fallopian tube disease in the nonpregnant patient","volume":"31","author":"Rezvani","year":"2011","journal-title":"Radiographics"},{"key":"2026010712223221800_bib27","doi-asserted-by":"publisher","first-page":"140310","DOI":"10.5402\/2011\/140310","article-title":"Endometriosis and ovarian cancer: a systematic review","volume":"2011","author":"Sayasneh","year":"2011","journal-title":"ISRN Obstetrics and Gynecology"},{"key":"2026010712223221800_bib28","doi-asserted-by":"publisher","first-page":"E805","DOI":"10.3390\/molecules22050805","article-title":"Identification and validation of SAA4 as a rheumatoid arthritis prescreening marker by liquid chromatography tandem-mass spectrometry","volume":"22","author":"Seok","year":"2017","journal-title":"Molecules"},{"key":"2026010712223221800_bib29","doi-asserted-by":"publisher","first-page":"1063","DOI":"10.1093\/humrep\/den078","article-title":"Global gene analysis of late secretory phase, eutopic endometrium does not provide the basis for a minimally invasive test of endometriosis","volume":"23","author":"Sherwin","year":"2008","journal-title":"Human Reproduction"},{"key":"2026010712223221800_bib30","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1111\/aogs.13082","article-title":"Endometriosis-associated infertility: aspects of pathophysiological mechanisms and treatment options","volume":"96","author":"Tanbo","year":"2017","journal-title":"Acta Obstetricia et Gynecologica Scandinavica"},{"key":"2026010712223221800_bib31","doi-asserted-by":"publisher","first-page":"960","DOI":"10.1074\/mcp.M400055-MCP200","article-title":"Integrated genomic and proteomic analyses of gene expression in Mammalian cells","volume":"3","author":"Tian","year":"2004","journal-title":"Molecular and Cellular Proteomics"},{"key":"2026010712223221800_bib32","doi-asserted-by":"publisher","first-page":"4983","DOI":"10.1021\/pr500384n","article-title":"Ovarian endometriosis signatures established through discovery and directed mass spectrometry analysis","volume":"13","author":"Vehmas","year":"2014","journal-title":"Journal of Proteome Research"},{"key":"2026010712223221800_bib33","doi-asserted-by":"publisher","first-page":"194","DOI":"10.1016\/j.jprot.2014.09.015","article-title":"Urine, peritoneal fluid and omental fat proteomes of reproductive age women: endometriosis-related changes and associations with endocrine disrupting chemicals","volume":"113","author":"Williams","year":"2015","journal-title":"Journal of Proteomics"},{"key":"2026010712223221800_bib34","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1038\/nmeth.1322","article-title":"Universal sample preparation method for proteome analysis","volume":"6","author":"Wisniewski","year":"2009","journal-title":"Nature Methods"},{"key":"2026010712223221800_bib35","doi-asserted-by":"publisher","first-page":"48","DOI":"10.1186\/s12958-018-0361-y","article-title":"Effects of pelvic endometriosis and adenomyosis on ciliary beat frequency and muscular contractions in the human fallopian tube","volume":"16","author":"Xia","year":"2018","journal-title":"Reproductive Biology and Endocrinology"},{"key":"2026010712223221800_bib36","doi-asserted-by":"publisher","first-page":"e3726","DOI":"10.7717\/peerj.3726","article-title":"Identification of reference genes in blood before and after entering the plateau for SYBR Green RT-qPCR studies","volume":"5","author":"Xiao","year":"2017","journal-title":"PeerJ"},{"key":"2026010712223221800_bib37","doi-asserted-by":"publisher","first-page":"2962","DOI":"10.1002\/pmic.201600315","article-title":"Exploring the oviductal fluid proteome by a lectin-based affinity approach","volume":"16","author":"Yu","year":"2016","journal-title":"Proteomics"},{"key":"2026010712223221800_bib38","doi-asserted-by":"publisher","first-page":"271ra9","DOI":"10.1126\/scitranslmed.3010626","article-title":"Dual suppression of estrogenic and inflammatory activities for targeting of endometriosis","volume":"7","author":"Zhao","year":"2015","journal-title":"Science Translational Medicine"}],"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rep.bioscientifica.com\/view\/journals\/rep\/159\/5\/REP-19-0587.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/159\/5\/601\/64126150\/rep-19-0587.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/159\/5\/601\/64126150\/rep-19-0587.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T17:22:40Z","timestamp":1767806560000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/159\/5\/601\/8235514"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,1]]},"references-count":38,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5,1]]}},"URL":"https:\/\/doi.org\/10.1530\/rep-19-0587","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,5,1]]},"published":{"date-parts":[[2020,5,1]]}}}