{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T02:22:14Z","timestamp":1785378134870,"version":"3.55.0"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Asthenozoospermia (AS), defined as low-motility spermatozoa in the ejaculate, is a frequent cause of human male infertility. DJ-1 (also known as PARK7), a protein highly associated with male sterility, binds to the mitochondrial complex I subunit to protect mitochondrial function. However, its involvement in spermatogenesis has not been fully elucidated. Previously, the levels of DJ-1 were shown to be significantly decreased in testicular tissues of rats with ornidazole (ORN)-induced AS. Here, we used a rat model to investigate the localization and expression levels of DJ-1 and its interacting NDUFS3 and NDUFA4 mitochondrial complex I subunits, as well as AS-induced metabolic alterations in testicular tissues. ORN significantly reduced the levels of DJ-1 in the nucleus of secondary spermatocytes, while increasing the expression of NDUFS3 in the cytoplasm of primary spermatocytes. Further, NDUFA4 showed higher expression after treatment with ORN. The principal ORN-induced changes in metabolic small molecules related to the accumulation of glucose, glutamine, and N-acetyl aspartate, enhancement of purine pathway, increase of the phosphatidic acid (PA) (18:0\/18:1), phosphatidylethanolamine (PE) (16:0\/18:1), and PA (18:0\/20:4) lipid metabolites, and imbalance in the concentrations of Na+ and K+. However, we did not observe any abnormalities of certain small metabolic molecules and metal ions in semen samples from patients with AS. In conclusion, these results suggest that DJ-1 deficiency in testicular tissues might be closely related to the localization of NDUFS3 and content of NDUFA4, thus causing abnormalities in the mitochondrial energy metabolism and multiple other metabolic pathways.<\/jats:p>","DOI":"10.1530\/rep-20-0097","type":"journal-article","created":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T11:36:43Z","timestamp":1601293003000},"page":"931-941","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":11,"title":["DJ-1 deficiency causes metabolic abnormality in ornidazole-induced asthenozoospermia"],"prefix":"10.1093","volume":"160","author":[{"given":"Yi","family":"Sun","sequence":"first","affiliation":[{"name":"National Key Research Laboratory of Natural and Biomimetic Drugs , Peking University, Beijing, P eople\u2019s Republic of China"},{"name":"Department of Molecular and Cellular Pharmacology , School of Pharmaceutical Sciences, Peking University, Beijing, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinping","family":"Sun","sequence":"additional","affiliation":[{"name":"Clinical Laboratory , Peking University International Hospital, Beijing, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lianming","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Urology , Peking University Third Hospital, Beijing, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhe","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology , Peking University Third Hospital, Beijing, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yupeng","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Research Laboratory of Natural and Biomimetic Drugs , Peking University, Beijing, P eople\u2019s Republic of China"},{"name":"Department of Molecular and Cellular Pharmacology , School of Pharmaceutical Sciences, Peking University, Beijing, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenfeng","family":"Dai","sequence":"additional","affiliation":[{"name":"National Key Research Laboratory of Natural and Biomimetic Drugs , Peking University, Beijing, P eople\u2019s Republic of China"},{"name":"Department of Molecular and Cellular Pharmacology , School of Pharmaceutical Sciences, Peking University, Beijing, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhao","sequence":"additional","affiliation":[{"name":"National Key Research Laboratory of Natural and Biomimetic Drugs , Peking University, Beijing, P eople\u2019s Republic of China"},{"name":"Department of Molecular and Cellular Pharmacology , School of Pharmaceutical Sciences, Peking 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