{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T14:35:40Z","timestamp":1792074940103,"version":"4.4.0"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T00:00:00Z","timestamp":1606780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2020,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Obesity is a transgenerational epigenetic metabolic disturbance. Although the diet-induced obese (DIO) zebrafish model is well established, reproductive parameters and changes in offspring have not yet been evaluated. Thus, the aim of this study was to evaluate possible changes in reproductive parameters, embryos and offspring (F1) generated by the reproduction of diet-induced obese males and females. The adult zebrafish were divided into two groups: one group receiving a balanced diet (control group) and the other group was overfed (DIO group) . The dietary protocol was maintained for 8 weeks. During this period, males and females in the same group were stimulated through a weekly reproduction protocol. To verify parental obesity, body weight, blood glucose, triglyceride, the hepatosomatic and gonadosomatic index and adipose tissue morphometry evaluations were carried out. Reproductive parameters were evaluated through ovarian and oocyte maturation stage, total spawning, fertility and fertilization index. To verify possible changes caused by parenteral obesity, all offspring were kept in separate groups in correspondence with their parents and were fed a control diet. Plasma glucose, triglycerides, mortality rate, hatching, and deformities were determined. After 8 weeks under the diet protocol, the DIO group exhibited characteristic obesity alterations, displaying significant increases in body mass and hepatosomatic and gonadosomatic indices, hyperglycemia and visceral and subcutaneous adipocyte hypertrophy. In addition, high mortality rates, morphologic deformities and high plasmatic glucose and triglyceride levels, with 100% mortality at 60 dpf, were observed for the offspring. Therefore, obesity induction in adults led to negative effects on their offspring, with a high occurrence of deformities and mortality.<\/jats:p>","DOI":"10.1530\/rep-20-0119","type":"journal-article","created":{"date-parts":[[2020,9,4]],"date-time":"2020-09-04T06:59:37Z","timestamp":1599202777000},"page":"833-842","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":20,"title":["Obesity induction in adult zebrafish leads to negative reproduction and offspring effects"],"prefix":"10.1093","volume":"160","author":[{"given":"B\u00e1rbara do Carmo Rodrigues","family":"Virote","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine , Central Animal Housing, Federal University of Lavras, Lavras, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amanda Maria Siqueira","family":"Moreira","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine , Central Animal Housing, Federal University of Lavras, Lavras, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Gilmar da","family":"Silva Souza","sequence":"additional","affiliation":[{"name":"Department of Animal Science , Central Animal Housing, Federal University of Lavras, Lavras, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u00e1ssia Fl\u00e1via Dias","family":"Castro","sequence":"additional","affiliation":[{"name":"Department of Animal Science , Central Animal Housing, Federal University of Lavras, Lavras, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naiara","family":"Melo","sequence":"additional","affiliation":[{"name":"Department of Animal Science , Central Animal Housing, Federal University of Lavras, Lavras, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William Franco","family":"Carneiro","sequence":"additional","affiliation":[{"name":"Department of Animal Science , Central Animal Housing, Federal University of Lavras, Lavras, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina Delarete","family":"Drummond","sequence":"additional","affiliation":[{"name":"Department of Health Science , Federal University of Lavras, Lavras, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 Rodrigues da Cunha Barreto","family":"Vianna","sequence":"additional","affiliation":[{"name":"Department of Biosciences , Federal University of Paran\u00e1, Palotina, Paran\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis David Solis","family":"Murgas","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine , Central Animal Housing, Federal University of Lavras, Lavras, Minas Gerais, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,12,1]]},"reference":[{"key":"2026010712240762400_bib1","doi-asserted-by":"publisher","DOI":"10.3791\/4196","article-title":"Regular care and maintenance of a zebrafish (Danio rerio) laboratory: an introduction","volume":"69","author":"Avdesh","year":"2012","journal-title":"Journal of Visualized Experiments"},{"key":"2026010712240762400_bib2","doi-asserted-by":"publisher","first-page":"1086","DOI":"10.1016\/j.envpol.2018.11.032","article-title":"Examining the responses of the zebrafish (Danio rerio) gastrointestinal system to the suspected obesogen diet ethylhexyl phthalate","volume":"245","author":"Buerger","year":"2019","journal-title":"Environmental Pollution"},{"key":"2026010712240762400_bib3","doi-asserted-by":"publisher","first-page":"595","DOI":"10.1016\/j.scitotenv.2018.07.331","article-title":"Parental exposure to a zoxystrobin causes developmental effects and disrupts gene expression in F1 embryonic zebrafish (Danio rerio)","volume":"646","author":"Cao","year":"2019","journal-title":"Science of the Total Environment"},{"key":"2026010712240762400_bib4","doi-asserted-by":"publisher","first-page":"140","DOI":"10.1016\/j.etap.2018.06.011","article-title":"Anxiety-associated behavior and genotoxicity found in adult Danio rerio exposed to tebuconazole-based commercial product","volume":"62","author":"Castro","year":"2018","journal-title":"Environmental Toxicology and Pharmacology"},{"key":"2026010712240762400_bib5","author":"Chen"},{"key":"2026010712240762400_bib6","doi-asserted-by":"publisher","first-page":"789","DOI":"10.1590\/S0100-204X2005000800009","article-title":"Indicadores quantitativos da biologia reprodutiva de f\u00eameas de piau-vermelho no Rio Para\u00edba do Sul","volume":"40","author":"Costa","year":"2005","journal-title":"Pesquisa Agropecu\u00e1ria Brasileira"},{"key":"2026010712240762400_bib7","first-page":"1565","article-title":"Oogonial proliferation. 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