{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T15:21:47Z","timestamp":1792596107965,"version":"4.5.0"},"reference-count":63,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2021,1,1]],"date-time":"2021-01-01T00:00:00Z","timestamp":1609459200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2021,1,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Preimplantation embryos are particularly vulnerable to environmental perturbations, including those related to assisted reproductive technologies. Invasive embryo manipulations, such as blastomere biopsy, are applied worldwide in clinical settings for preimplantation genetic testing. Mouse models have previously shown that blastomere biopsy may be associated with altered phenotypes in adult offspring. The aim of the present study was to investigate the specific contribution of blastomere removal to the physiological, behavioral, and molecular regulators of energy homeostasis, as compared to sham manipulation (re-introducing the blastomere into the embryo after its removal) and in vitro culture. Mice derived from 8-cell embryos subjected to blastomere removal displayed: (i) higher body weight and adiposity, (ii) increased food intake and sucrose preference, (iii) decreased time of immobility in the tail suspension test, and (iv) resistance to weight loss after social isolation or following 3 days of physical exercise \u2013 compared to mice derived from sham biopsy or from in vitro-cultured embryos. Mice generated after blastomere removal also had increased circulating leptin and leptin gene expression in adipose tissue, as well as increased ghrelin receptor gene expression in the hypothalamus, compared to control mice. The effects of blastomere biopsy on offspring phenotype were sexually dimorphic, with females not being affected. These results indicate that blastomere deprivation, rather than other perturbations of the blastomere biopsy procedure, programs male embryos to develop physiological, behavioral, and molecular dysregulation of energy homeostasis, leading to postnatal obesity.<\/jats:p>","DOI":"10.1530\/rep-20-0253","type":"journal-article","created":{"date-parts":[[2020,10,19]],"date-time":"2020-10-19T11:46:30Z","timestamp":1603107990000},"page":"61-72","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":3,"title":["Blastomere removal affects homeostatic control leading to obesity in male mice"],"prefix":"10.1093","volume":"161","author":[{"given":"Magdalena","family":"Kotlarska","sequence":"first","affiliation":[{"name":"Department of Experimental Embryology , Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Jastrz\u0119biec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawid","family":"Winiarczyk","sequence":"additional","affiliation":[{"name":"Department of Experimental Embryology , Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Jastrz\u0119biec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wies\u0142awa","family":"Florek","sequence":"additional","affiliation":[{"name":"Department of Experimental Embryology , Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Jastrz\u0119biec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Zi\u0119tek","sequence":"additional","affiliation":[{"name":"Department of Experimental Embryology , Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Jastrz\u0119biec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jolanta","family":"P\u0119czkowicz-Szyszka","sequence":"additional","affiliation":[{"name":"Department of Experimental Embryology , Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Jastrz\u0119biec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian Mateusz","family":"Stankiewicz","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology , Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Jastrz\u0119biec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafa\u0142 Rados\u0142aw","family":"Starzy\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology , Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Jastrz\u0119biec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberta","family":"Arena","sequence":"additional","affiliation":[{"name":"Department of Experimental Embryology , Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Jastrz\u0119biec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaspare","family":"Drago","sequence":"additional","affiliation":[{"name":"National Research Council of Italy , Institute of Biomedical Research and Innovation, Laboratory of Environmental Epidemiology, Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvestre","family":"Sampino","sequence":"additional","affiliation":[{"name":"Department of Experimental Embryology , Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Jastrz\u0119biec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacek Andrzej","family":"Modlinski","sequence":"additional","affiliation":[{"name":"Department of Experimental Embryology , Institute of Genetics and Animal Biotechnology of the Polish Academy of Sciences, Jastrz\u0119biec, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,1,1]]},"reference":[{"key":"2026010712050563500_bib1","doi-asserted-by":"publisher","first-page":"R1","DOI":"10.1530\/REP-11-0489","article-title":"Sex differences in developmental programming models","volume":"145","author":"Aiken","year":"2013","journal-title":"Reproduction"},{"key":"2026010712050563500_bib2","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clinical Chemistry"},{"key":"2026010712050563500_bib3","doi-asserted-by":"publisher","first-page":"1682","DOI":"10.1210\/jc.2007-2432","article-title":"Cardiometabolic differences in children born after in vitro fertilization: follow-up study","volume":"93","author":"Ceelen","year":"2008","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"key":"2026010712050563500_bib4","doi-asserted-by":"publisher","first-page":"292","DOI":"10.1056\/NEJM199602013340503","article-title":"Serum immunoreactive-leptin concentrations in normal-weight and obese humans","volume":"334","author":"Considine","year":"1996","journal-title":"New England Journal of Medicine"},{"key":"2026010712050563500_bib5","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1016\/j.neubiorev.2005.03.009","article-title":"The tail suspension test as a model for assessing antidepressant activity: review of pharmacological and genetic studies in mice","volume":"29","author":"Cryan","year":"2005","journal-title":"Neuroscience and Biobehavioral Reviews"},{"key":"2026010712050563500_bib6","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1016\/0092-8674(83)90379-3","article-title":"Identification and purification of a cell surface glycoprotein mediating intercellular adhesion in embryonic and adult tissue","volume":"34","author":"Damsky","year":"1983","journal-title":"Cell"},{"key":"2026010712050563500_bib7","doi-asserted-by":"publisher","first-page":"1803","DOI":"10.1056\/NEJMoa1008095","article-title":"Reproductive technologies and the risk of birth defects","volume":"366","author":"Davies","year":"2012","journal-title":"New England Journal of Medicine"},{"key":"2026010712050563500_bib8","doi-asserted-by":"publisher","first-page":"1974","DOI":"10.1093\/humrep\/dex265","article-title":"ESHRE PGD Consortium data collection XIV-XV: cycles from January 2011 to December 2012 with pregnancy follow-up to October 2013","volume":"32","author":"De Rycke","year":"2017","journal-title":"Human Reproduction"},{"key":"2026010712050563500_bib9","doi-asserted-by":"publisher","first-page":"288","DOI":"10.1093\/humrep\/der360","article-title":"Neonatal follow-up of 995 consecutively born children after embryo biopsy for PGD","volume":"27","author":"Desmyttere","year":"2012","journal-title":"Human Reproduction"},{"key":"2026010712050563500_bib10","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/0012-1606(75)90262-6","article-title":"Cell shape and membrane changes in the eight-cell mouse embryo: prerequisites for morphogenesis of the blastocyst","volume":"47","author":"Ducibella","year":"1975","journal-title":"Developmental Biology"},{"key":"2026010712050563500_bib11","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1093\/humrep\/dep456","article-title":"Effect of in vitro culture of human embryos on birthweight of newborns","volume":"25","author":"Dumoulin","year":"2010","journal-title":"Human Reproduction"},{"key":"2026010712050563500_bib12","doi-asserted-by":"publisher","first-page":"10","DOI":"10.1016\/j.cmet.2010.06.001","article-title":"Assessment of feeding behavior in laboratory mice","volume":"12","author":"Ellacott","year":"2010","journal-title":"Cell Metabolism"},{"key":"2026010712050563500_bib13","doi-asserted-by":"publisher","first-page":"2162","DOI":"10.1093\/humrep\/dey314","article-title":"Non-invasive pre-implantation genetic testing of human embryos: an emerging concept","volume":"33","author":"Farra","year":"2018","journal-title":"Human Reproduction"},{"key":"2026010712050563500_bib14","doi-asserted-by":"publisher","first-page":"1677","DOI":"10.2174\/1568026618666181115095920","article-title":"Depression assessment in clinical trials and pre-clinical tests: a critical review","volume":"18","author":"Ferreira","year":"2018","journal-title":"Current Topics in Medicinal Chemistry"},{"key":"2026010712050563500_bib15","doi-asserted-by":"publisher","first-page":"1311","DOI":"10.1038\/nm1295-1311","article-title":"Leptin levels reflect body lipid content in mice: evidence for diet-induced resistance to leptin action","volume":"1","author":"Frederich","year":"1995","journal-title":"Nature Medicine"},{"key":"2026010712050563500_bib16","doi-asserted-by":"publisher","first-page":"8","DOI":"10.1016\/j.mce.2009.02.015","article-title":"Sexual dimorphism in environmental epigenetic programming","volume":"304","author":"Gabory","year":"2009","journal-title":"Molecular and Cellular Endocrinology"},{"key":"2026010712050563500_bib17","doi-asserted-by":"publisher","DOI":"10.1186\/2042-6410-4-5","article-title":"Placental contribution to the origins of sexual dimorphism in health and diseases: sex chromosomes and epigenetics","volume":"4","author":"Gabory","year":"2013","journal-title":"Biology of Sex Differences"},{"key":"2026010712050563500_bib18","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0014398","article-title":"Sex- and diet-specific changes of imprinted gene expression and DNA methylation in mouse placenta under a high-fat diet","volume":"5","author":"Gallou-Kabani","year":"2010","journal-title":"PLoS ONE"},{"key":"2026010712050563500_bib19","doi-asserted-by":"publisher","first-page":"1899","DOI":"10.1002\/jcb.26351","article-title":"A potential high risk for fatty liver disease was found in mice generated after assisted reproductive techniques","volume":"119","author":"Gu","year":"2018","journal-title":"Journal of Cellular Biochemistry"},{"key":"2026010712050563500_bib20","doi-asserted-by":"publisher","first-page":"768","DOI":"10.1038\/344768a0","article-title":"Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification","volume":"344","author":"Handyside","year":"1990","journal-title":"Nature"},{"key":"2026010712050563500_bib21","doi-asserted-by":"publisher","first-page":"330","DOI":"10.1093\/humupd\/dmt006","article-title":"Assisted reproductive technology and birth defects: a systematic review and meta-analysis","volume":"19","author":"Hansen","year":"2013","journal-title":"Human Reproduction Update"},{"key":"2026010712050563500_bib22","doi-asserted-by":"publisher","first-page":"244","DOI":"10.1093\/humupd\/dmt002","article-title":"The longer-term health outcomes for children born as a result of IVF treatment. Part II \u2013 Mental health and development outcomes","volume":"19","author":"Hart","year":"2013","journal-title":"Human Reproduction Update"},{"key":"2026010712050563500_bib23","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1093\/humupd\/dms062","article-title":"The longer-term health outcomes for children born as a result of IVF treatment. Part I \u2013 General health outcomes","volume":"19","author":"Hart","year":"2013","journal-title":"Human Reproduction Update"},{"key":"2026010712050563500_bib24","doi-asserted-by":"publisher","first-page":"1995","DOI":"10.1007\/s10815-018-1286-2","article-title":"Perinatal follow-up of children born after preimplantation genetic diagnosis between 1995 and 2014","volume":"35","author":"Heijligers","year":"2018","journal-title":"Journal of Assisted Reproduction and Genetics"},{"key":"2026010712050563500_bib25","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1136\/bmj.37957.560278.EE","article-title":"Perinatal outcome of singletons and twins after assisted conception: a systematic review of controlled studies","volume":"328","author":"Helmerhorst","year":"2004","journal-title":"BMJ"},{"key":"2026010712050563500_bib26","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/j.bbr.2017.02.040","article-title":"Ghrelin alleviates anxiety- and depression-like behaviors induced by chronic unpredictable mild stress in rodents","volume":"326","author":"Huang","year":"2017","journal-title":"Behavioural Brain Research"},{"key":"2026010712050563500_bib27","first-page":"239","article-title":"The role of cell adhesion in the synchronization and orientation of polarization in 8-cell mouse blastomeres","volume":"93","author":"Johnson","year":"1986","journal-title":"Journal of Embryology and Experimental Morphology"},{"key":"2026010712050563500_bib28","doi-asserted-by":"publisher","first-page":"4352","DOI":"10.1210\/jc.2006-0701","article-title":"Serum insulin-like growth factor-I (IGF-I) and growth in children born after assisted reproduction","volume":"91","author":"Kai","year":"2006","journal-title":"Journal of Clinical Endocrinology and Metabolism"},{"key":"2026010712050563500_bib29","doi-asserted-by":"publisher","first-page":"2219","DOI":"10.1093\/humrep\/dew156","article-title":"Influence of embryo culture medium (G5 and HTF) on pregnancy and perinatal outcome after IVF: a multicenter RCT","volume":"31","author":"Kleijkers","year":"2016","journal-title":"Human Reproduction"},{"key":"2026010712050563500_bib30","doi-asserted-by":"publisher","first-page":"2328","DOI":"10.1093\/humrep\/deg445","article-title":"Growth, psychomotor development and morbidity up to 3 years of age in children born after IVF","volume":"18","author":"Koivurova","year":"2003","journal-title":"Human Reproduction"},{"key":"2026010712050563500_bib31","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1093\/humrep\/dex337","article-title":"Developmental outcome of 9-year-old children born after PGS: follow-up of a randomized trial","volume":"33","author":"Kuiper","year":"2018","journal-title":"Human Reproduction"},{"key":"2026010712050563500_bib32","doi-asserted-by":"publisher","first-page":"851","DOI":"10.1016\/0092-8674(87)90108-5","article-title":"Gap junctional communication and compaction during preimplantation stages of mouse development","volume":"51","author":"Lee","year":"1987","journal-title":"Cell"},{"key":"2026010712050563500_bib33","doi-asserted-by":"publisher","first-page":"399","DOI":"10.1016\/0092-8674(79)90059-x","article-title":"Gap junctional communication in the preimplantation mouse embryo","volume":"18","author":"Lo","year":"1979","journal-title":"Cell"},{"key":"2026010712050563500_bib34","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1016\/j.fertnstert.2015.11.018","article-title":"Preimplantation genetic testing: polar bodies, blastomeres, trophectoderm cells, or blastocoelic fluid?","volume":"105","author":"Magli","year":"2016","journal-title":"Fertility and Sterility"},{"key":"2026010712050563500_bib35","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/j.fertnstert.2011.04.081","article-title":"Mental, psychomotor, neurologic, and behavioral outcomes of 2-year-old children born after preimplantation genetic screening: follow-up of a randomized controlled trial","volume":"96","author":"Middelburg","year":"2011","journal-title":"Fertility and Sterility"},{"key":"2026010712050563500_bib36","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.170210","article-title":"The first cell-fate decision of mouse preimplantation embryo development: integrating cell position and polarity","volume":"7","author":"Mihajlovi\u0107","year":"2017","journal-title":"Open Biology"},{"key":"2026010712050563500_bib37","author":"Nagy","year":"2003","journal-title":"Manipulating the Mouse Embryo: A Laboratory Manual"},{"key":"2026010712050563500_bib38","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/0140-6736(92)92425-f","article-title":"Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte","volume":"340","author":"Palermo","year":"1992","journal-title":"Lancet"},{"key":"2026010712050563500_bib39","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1242\/dev.01602","article-title":"Four-cell stage mouse blastomeres have different developmental properties","volume":"132","author":"Piotrowska-Nitsche","year":"2005","journal-title":"Development"},{"key":"2026010712050563500_bib40","doi-asserted-by":"publisher","first-page":"458","DOI":"10.1080\/09297049.2015.1014900","article-title":"Developmental neuropsychological assessment of 4- to 5-year-old children born following preimplantation genetic diagnosis (PGD): a pilot study","volume":"22","author":"Sacks","year":"2016","journal-title":"Child Neuropsychology"},{"key":"2026010712050563500_bib41","doi-asserted-by":"publisher","first-page":"1693","DOI":"10.1016\/j.fertnstert.2009.09.049","article-title":"Absence of insulin resistance and low-grade inflammation despite early metabolic syndrome manifestations in children born after in vitro fertilization","volume":"94","author":"Sakka","year":"2010","journal-title":"Fertility and Sterility"},{"key":"2026010712050563500_bib42","doi-asserted-by":"publisher","first-page":"1875","DOI":"10.1093\/humrep\/deu145","article-title":"Effects of blastomere biopsy on post-natal growth and behavior in mice","volume":"29","author":"Sampino","year":"2014","journal-title":"Human Reproduction"},{"key":"2026010712050563500_bib43","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-66222-3","article-title":"Placental metabolomics for assessment of sex-specific differences in fetal development during normal gestation","volume":"10","author":"Saoi","year":"2020","journal-title":"Scientific Reports"},{"key":"2026010712050563500_bib62","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.1093\/molehr\/gau072","article-title":"Single blastomere removal from murine embryos is associated with activation of matrix metalloproteinases and Janus kinase\/signal transducers and activators of transcription pathways of placental inflammations","author":"Sato","year":"2014","journal-title":"Molecular Human Reproduction 20"},{"key":"2026010712050563500_bib44","doi-asserted-by":"publisher","first-page":"1508","DOI":"10.1093\/humrep\/det073","article-title":"The effect of preimplantation genetic screening on neurological, cognitive and behavioural development in 4-year-old children: follow-up of a RCT","volume":"28","author":"Schendelaar","year":"2013","journal-title":"Human Reproduction"},{"key":"2026010712050563500_bib45","doi-asserted-by":"publisher","first-page":"807","DOI":"10.1530\/REP-17-0514","article-title":"Embryo cell allocation patterns are not altered by biopsy but can be linked with further development","volume":"154","author":"Sepulveda-Rincon","year":"2017","journal-title":"Reproduction"},{"key":"2026010712050563500_bib46","doi-asserted-by":"publisher","first-page":"1312","DOI":"10.1016\/j.fertnstert.2016.07.1112","article-title":"Proof of concept: preimplantation genetic screening without embryo biopsy through analysis of cell-free DNA in spent embryo culture media","volume":"106","author":"Shamonki","year":"2016","journal-title":"Fertility and Sterility"},{"key":"2026010712050563500_bib47","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(78)92957-4","article-title":"Birth after the reimplantation of a human embryo","volume":"2","author":"Steptoe","year":"1978","journal-title":"Lancet"},{"key":"2026010712050563500_bib48","doi-asserted-by":"publisher","first-page":"234","DOI":"10.1016\/j.theriogenology.2012.08.007","article-title":"Biopsy of embryos produced by in vitro fertilization affects development in C57BL\/6 mouse strain","volume":"79","author":"Sugawara","year":"2013","journal-title":"Theriogenology"},{"key":"2026010712050563500_bib49","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1095\/biolreprod.111.097444","article-title":"Blastomere removal from cleavage-stage mouse embryos alters steroid metabolism during pregnancy","volume":"87","author":"Sugawara","year":"2012","journal-title":"Biology of Reproduction"},{"key":"2026010712050563500_bib50","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0021208","article-title":"Deterministic and stochastic allele specific gene expression in single mouse blastomeres","volume":"6","author":"Tang","year":"2011","journal-title":"PLoS ONE"},{"key":"2026010712050563500_bib51","doi-asserted-by":"publisher","first-page":"190","DOI":"10.1016\/j.ydbio.2010.09.022","article-title":"Individual blastomeres of 16- and 32-cell mouse embryos are able to develop into foetuses and mice","volume":"348","author":"Tarkowski","year":"2010","journal-title":"Developmental Biology"},{"key":"2026010712050563500_bib52","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1007\/s12519-012-0374-0","article-title":"Psychomotor development of children born after preimplantation genetic diagnosis and parental stress evaluation","volume":"8","author":"Thomaidis","year":"2012","journal-title":"World Journal of Pediatrics"},{"key":"2026010712050563500_bib53","doi-asserted-by":"publisher","first-page":"214","DOI":"10.1038\/nature05458","article-title":"Histone arginine methylation regulates pluripotency in the early mouse embryo","volume":"445","author":"Torres-Padilla","year":"2007","journal-title":"Nature"},{"key":"2026010712050563500_bib54","doi-asserted-by":"publisher","first-page":"1331.e1","DOI":"10.1016\/j.fertnstert.2012.02.053","article-title":"Birth defects in children conceived by in vitro fertilization and intracytoplasmic sperm injection: a meta-analysis","volume":"97","author":"Wen","year":"2012","journal-title":"Fertility and Sterility"},{"key":"2026010712050563500_bib55","doi-asserted-by":"publisher","first-page":"1122","DOI":"10.1093\/humrep\/dev036","article-title":"Psychosocial development of full term singletons, born after preimplantation genetic diagnosis (PGD) at preschool age and family functioning: a prospective case-controlled study and multi-informant approach","volume":"30","author":"Winter","year":"2015","journal-title":"Human Reproduction"},{"key":"2026010712050563500_bib56","doi-asserted-by":"publisher","first-page":"1761","DOI":"10.1007\/s00018-013-1466-2","article-title":"Blastomere biopsy influences epigenetic reprogramming during early embryo development, which impacts neural development and function in resulting mice","volume":"71","author":"Wu","year":"2014","journal-title":"Cellular and Molecular Life Sciences"},{"key":"2026010712050563500_bib57","doi-asserted-by":"publisher","first-page":"2634","DOI":"10.1210\/en.2011-0004","article-title":"Impaired CNS leptin action is implicated in depression associated with obesity","volume":"152","author":"Yamada","year":"2011","journal-title":"Endocrinology"},{"key":"2026010712050563500_bib63","doi-asserted-by":"publisher","first-page":"25023","DOI":"10.1038\/srep25023","article-title":"Blastomere removal from cleavage-stage mouse embryos alters placental function, which is associated with placental oxidative stress and inflammation","volume":"6","author":"Yao","year":"2016","journal-title":"Scientific Reports"},{"key":"2026010712050563500_bib58","doi-asserted-by":"publisher","first-page":"4837","DOI":"10.3390\/ijms15034837","article-title":"The growth hormone secretagogue receptor: its intracellular signaling and regulation","volume":"15","author":"Yin","year":"2014","journal-title":"International Journal of Molecular Sciences"},{"key":"2026010712050563500_bib59","doi-asserted-by":"publisher","first-page":"1490","DOI":"10.1074\/mcp.M800273-MCP200","article-title":"Evaluation of blastomere biopsy using a mouse model indicates the potential high risk of neurodegenerative disorders in the offspring","volume":"8","author":"Yu","year":"2009","journal-title":"Molecular and Cellular Proteomics"},{"key":"2026010712050563500_bib60","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1007\/s00441-013-1728-1","article-title":"Preimplantation genetic diagnosis (PGD) influences adrenal development and response to cold stress in resulting mice","volume":"354","author":"Zeng","year":"2013","journal-title":"Cell and Tissue Research"},{"key":"2026010712050563500_bib100","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.113.109926","article-title":"Aberrant epigenetic modification in murine brain tissues of offspring from preimplantation genetic diagnosis blastomere biopsies","volume":"89","author":"Zhao","year":"2013","journal-title":"Biology of Reproduction"}],"container-title":["Reproduction"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/rep.bioscientifica.com\/view\/journals\/rep\/161\/1\/REP-20-0253.xml","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/161\/1\/61\/64063185\/rep-20-0253.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/reproduction\/article-pdf\/161\/1\/61\/64063185\/rep-20-0253.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T17:05:41Z","timestamp":1767805541000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/reproduction\/article\/161\/1\/61\/8216764"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,1]]},"references-count":63,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2021,1,1]]}},"URL":"https:\/\/doi.org\/10.1530\/rep-20-0253","relation":{},"ISSN":["1470-1626","1741-7899"],"issn-type":[{"value":"1470-1626","type":"print"},{"value":"1741-7899","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,1,1]]},"published":{"date-parts":[[2021,1,1]]}}}