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We hypothesized that the nature and intensity of reciprocal embryo-endometrium interactions depend on the extent of their physical proximity. Bovine endometrial epithelial cells (bEECs) and morulae were co-cultured in juxtacrine (Contact+) or non-juxtacrine (Contact\u2212) apposition. Co-culture with bEECs improved blastocyst rates on day 7.5, regardless of juxtaposition. Contact+ regulated transcription of 1797 endometrial genes vs only 230 in the Contact\u2212 group compared to their control (no embryos) counterparts. A subset of 50 overlapping differentially expressed genes (DEGs) defined embryo-induced effects on bEEC transcriptome irrespective of juxtaposition. Functional analysis revealed pathways associated with interferon signaling and prostanoid biosynthesis. A total of 175 genes displayed a graded expression level depending on Contact+ or Contact\u2212. These genes were involved in interferon-related and antigen presentation pathways. Biological processes enriched exclusively in Contact+ included regulation of cell cycle and sex-steroid biosynthesis. We speculate that, in vivo, embryonic signals fine-tune the function of surrounding cells to ultimately maximize pregnancy success.<\/jats:p>","DOI":"10.1530\/rep-20-0344","type":"journal-article","created":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T14:44:02Z","timestamp":1600353842000},"page":"887-903","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":24,"title":["Molecular interactions at the bovine embryo\u2013endometrial epithelium interface"],"prefix":"10.1093","volume":"160","author":[{"given":"Mariana","family":"Sponchiado","sequence":"first","affiliation":[{"name":"Department of Veterinary Sciences , Gamete Research Centre, University of Antwerp, Wilrijk, Belgium"},{"name":"Department of Animal Reproduction , School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, Pirassununga, S\u00e3o Paulo, Brazil"},{"name":"Department of Animal Sciences , University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8498-8903","authenticated-orcid":false,"given":"Waleed F A","family":"Marei","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences , Gamete Research Centre, University of Antwerp, Wilrijk, Belgium"},{"name":"Department of Theriogenology , Faculty of Veterinary Medicine, Cairo University, Giza, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerrit T S","family":"Beemster","sequence":"additional","affiliation":[{"name":"Department of Biology , Laboratory for Integrated Plant Physiology Research (IMPRES), University of Antwerp, Antwerp, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter E J","family":"Bols","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences , Gamete Research Centre, University of Antwerp, Wilrijk, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario","family":"Binelli","sequence":"additional","affiliation":[{"name":"Department of Animal Reproduction , School of Veterinary Medicine and Animal Science, University of S\u00e3o Paulo, Pirassununga, S\u00e3o Paulo, Brazil"},{"name":"Department of Animal Sciences , University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo L M R","family":"Leroy","sequence":"additional","affiliation":[{"name":"Department of Veterinary Sciences , Gamete Research Centre, University of Antwerp, Wilrijk, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,12,1]]},"reference":[{"key":"2026010712231242100_bib1","doi-asserted-by":"publisher","first-page":"1657","DOI":"10.2527\/jas1984.5961657x","article-title":"In vitro development of porcine embryos in coculture with endometrial cell monolayers or culture 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