{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T13:14:23Z","timestamp":1768742063573,"version":"3.49.0"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"4","content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2022,10,1]]},"abstract":"<jats:sec>\n                    <jats:title>In brief<\/jats:title>\n                    <jats:p>Changes in the endometrium prior to implantation may be critical in predicting pregnancy outcomes. This study shows that the endocrine system directs positional changes in CCR7+ cells before implantation, which may be critical for developing maternal tolerance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Suppression of the maternal immune system is vital for the implantation of the semi-allogeneic embryo. Although progress in understanding the dialogue between mother and embryo has been made, key interactions between maternal immune cells, hormones, and chemokines remain elusive. Uterine expression of the C-C chemokine receptor type 7 (CCR7) could recruit T regulatory cells and facilitate localized immune suppression. To test this concept,\n                      <jats:italic>Ccr7<\/jats:italic>\n                      mRNA and protein were assessed in uterine tissue.\n                      <jats:italic>Ccr7<\/jats:italic>\n                      mRNA expression peaked at day 4 in pregnant rat uteri and then declined at days 5 and 6. CCR7 protein showed similar quantitative changes. To test if female sex steroids affected the spatial distribution of CCR7-expressing cells, uteri from ovariectomized rats, progesterone-pretreated rats (2 mg daily), and progesterone-pretreated rats injected with estradiol (0.2 \u00b5g) were analyzed. Progesterone increased CCR7-positive (+) cells in the antimesometrial stroma. Progesterone and estradiol increased CCR7+ cells in the mesometrial stroma. Estradiol increased the density of cluster of differentiation 4 (CD4) positive cells in the mesometrial stromal region over progesterone alone. The density of cells expressing the T regulatory cell marker, forkhead box protein 3 (FOXP3), increased in the antimesometrial stroma in response to progesterone alone. Progesterone and estradiol increased FOXP3+ cells in the antimesometrial region of the stroma. Co-localization of CCR7, CD4, and FOXP3 in the stroma suggests CCR7+ cells are T regulatory cells. Polarization of CCR7+ cells in the endometrial stroma was an intrinsic response regulated by sex steroids and did not require the presence of an embryo.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/rep-22-0074","type":"journal-article","created":{"date-parts":[[2022,8,12]],"date-time":"2022-08-12T09:10:38Z","timestamp":1660295438000},"page":"183-193","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":2,"title":["Pregnancy preparation: redistribution of CCR7-positive cells in the rat uterus"],"prefix":"10.1093","volume":"164","author":[{"given":"Hannah","family":"Thomas","sequence":"first","affiliation":[{"name":"Department of Biology, Pittsburg State University, Pittsburg, Kansas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4918-1012","authenticated-orcid":true,"given":"Erick","family":"McCloskey","sequence":"additional","affiliation":[{"name":"Department of Biology, Pittsburg State University, Pittsburg, Kansas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9226-1253","authenticated-orcid":true,"given":"Virginia","family":"Rider","sequence":"additional","affiliation":[{"name":"Department of Biology, Pittsburg State University, Pittsburg, Kansas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","reference":[{"key":"bib1","series-title":"Medicina Clinica","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1016\/j.medcli.2014.01.033","article-title":"Regulatory T cells, maternal-foetal immune tolerance and recurrent miscarriage: new therapeutic challenging opportunities","volume":"144","author":"Alijotas-Reig","year":"2015","unstructured":"Alijotas-Reig JMelnychuk TGris JM2015Regulatory T cells, maternal-foetal immune tolerance and recurrent miscarriage: new therapeutic challenging opportunities. 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