{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T22:04:09Z","timestamp":1783634649131,"version":"3.55.0"},"reference-count":64,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2023,6,21]],"date-time":"2023-06-21T00:00:00Z","timestamp":1687305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,8,1]]},"abstract":"<jats:title>\u2003<\/jats:title>\n                  <jats:sec>\n                    <jats:title>In brief<\/jats:title>\n                    <jats:p>The concentration of progesterone through the estrous cycle modulates uterine function to affect the luminal metabolome. This paper reports that the dynamic changes in the bovine uterine luminal metabolome during diestrus are independent of the concentration of progesterone in the previous cycle.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>In cattle, the concentration of sex steroids modulates uterine function, which is reflected in the composition of the luminal metabolome. Ultimately, the uterine luminal metabolome influences embryonic growth and development. Our objectives were (i) to compare the luminal metabolome 4, 7, and 14 days after estrus of cows that were exposed to greater (HP4; n\u2009=\u200916) vs lower (LP4; n\u2009=\u200924) concentrations of progesterone before displaying estrus and ovulating spontaneously and (ii) to identify changes in the luminal concentration of metabolites across these time points. Luminal epithelial cells and fluid were collected using a cytology brush, and gene expression and metabolite concentrations were assessed by RNAseq and targeted mass spectrometry, respectively. Metabolome profile was similar between treatments within each of days 4, 7, and 14 (false discovery rate (FDR): \u2265 0.1). Concentrations of 53 metabolites changed, independent of treatment, across the diestrus. Metabolites were mostly lipids (40 out 53) and the greatest concentrations were at day 14 (FDR: \u2264 0.1). On day 7, the concentration of putrescine and the gene expression of ODC1, PAOX, SLC3A2, and SAT1 increased (P \u2264 0.05). On day 14, the concentration of 3 ceramides, 4 glucosylceramides, and 12 sphingomyelins and the expression of SGMS2 were increased, in addition to the concentration of choline and 20 phosphatidylcholines. Collectively, the post-estrus concentration of luminal metabolites changed dynamically, independent of the concentration of sex steroids on the previous cycle, and the greatest magnitude changes were on day 14 when lipid metabolism was the most enriched pathway.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/rep-22-0372","type":"journal-article","created":{"date-parts":[[2023,5,24]],"date-time":"2023-05-24T13:07:51Z","timestamp":1684933671000},"page":"99-116","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":8,"title":["Pre-estrus progesterone does not affect post-estrus luminal metabolome in cross-bred beef cows"],"prefix":"10.1093","volume":"166","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9189-0912","authenticated-orcid":false,"given":"Felipe A C C","family":"Silva","sequence":"first","affiliation":[{"name":"Department of Animal Sciences , University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago","family":"Martins","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences , University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Sponchiado","sequence":"additional","affiliation":[{"name":"Department of Physiological Sciences , University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia C","family":"Rocha","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences , University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia","family":"Ashrafi","sequence":"additional","affiliation":[{"name":"Metabolomics Department , Beaumont Research Institute, Royal Oak, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stewart F","family":"Graham","sequence":"additional","affiliation":[{"name":"Metabolomics Department , Beaumont Research Institute, Royal Oak, Michigan, USA"},{"name":"Oakland University-William Beaumont School of Medicine , Rochester, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ky G","family":"Pohler","sequence":"additional","affiliation":[{"name":"Department of Animal Science , Texas A&M University, College Station, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Pe\u00f1agaricano","sequence":"additional","affiliation":[{"name":"Department of Animal and Dairy Sciences , University of Wisconsin-Madison, Madison, Wisconsin, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7446-3831","authenticated-orcid":false,"given":"Angela M","family":"Gonella-Diaza","sequence":"additional","affiliation":[{"name":"North Florida Research and Education Center , University of 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