{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T22:30:19Z","timestamp":1785364219930,"version":"3.55.0"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2023,6,19]],"date-time":"2023-06-19T00:00:00Z","timestamp":1687132800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,8,1]]},"abstract":"<jats:title>\u2003<\/jats:title>\n                  <jats:sec>\n                    <jats:title>In brief<\/jats:title>\n                    <jats:p>Inappropriate uterine contractions are a matter of concern during pregnancy or menses. We identified the transient receptor potential melastatin 4 (TRPM4) ion channel as a new actor in mouse uterine contractions highlighting this protein as a potential pharmacological target for a better control of myometrial activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>Control of uterine contractions is of interest in the context of inappropriate myometrial activity during pregnancy and at time of delivery, but it is also a matter for menstrual pain. While several molecular determinants of myometrial contractions have been described, the complete distribution of roles to the various actors is far from understood. A key phenomenon is a variation in cytoplasmic Ca2+ which leads to the activation of calmodulin in smooth muscle and also in the phosphorylation of myosin allowing contraction. The Ca2+ \u2013 TRPM4 channel which is known to modulate Ca2+\u2013 fluxes in several cell types was shown to participate in vascular as well as detrusor muscle contraction. We thus designed a study to determine whether it also participates in myometrial contraction. Uterine rings were isolated from Trpm4+\/+ and Trpm4\u2212\/\u2212 non-pregnant adult mice and contractions were recorded using an isometric force transducer. In basal conditions, spontaneous contractions were similar in both groups. Application of 9-phenanthrol, a pharmacological TRPM4 inhibitor, dose-dependently reduced contraction parameters in Trpm4+\/+ rings with an IC50 around 2.10\u22126 mol\/L. The effect of 9-phenanthrol was significantly reduced in Trpm4\u2212\/\u2212 rings. The effect of oxytocin was tested and was found to be stronger in Trpm4+\/+ rings compared to Trpm4\u2212\/\u2212. Under a constant stimulation by oxytocin, 9-phenanthrol still reduced contraction parameters in Trpm4+\/+ rings with a smaller effect on Trpm4\u2212\/\u2212. Altogether it indicates that TRPM4 participates in uterine contractions in mice and may thus be evaluated as a new target to control such contractions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/rep-22-0484","type":"journal-article","created":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T08:47:27Z","timestamp":1684486047000},"page":"77-87","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":4,"title":["TRPM4 contribution in mouse uterine contractions"],"prefix":"10.1093","volume":"166","author":[{"given":"Alexandre","family":"Fouchet","sequence":"first","affiliation":[{"name":"Normandie Universit\u00e9 , UR 4650, Physiopathologie et Strat\u00e9gies d'Imagerie du Remodelage cardiovasculaire, GIP Cyceron, Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harlyne Mpweme","family":"Bangando","sequence":"additional","affiliation":[{"name":"Normandie Universit\u00e9 , UR 4650, Physiopathologie et Strat\u00e9gies d'Imagerie du Remodelage cardiovasculaire, GIP Cyceron, Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margaux","family":"Aize","sequence":"additional","affiliation":[{"name":"Normandie Universit\u00e9 , UR 4650, Physiopathologie et Strat\u00e9gies d'Imagerie du Remodelage cardiovasculaire, GIP Cyceron, Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe","family":"Simard","sequence":"additional","affiliation":[{"name":"Normandie Universit\u00e9 , UR 4650, Physiopathologie et Strat\u00e9gies d'Imagerie du Remodelage cardiovasculaire, GIP Cyceron, Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0276-8884","authenticated-orcid":false,"given":"Romain","family":"Guinamard","sequence":"additional","affiliation":[{"name":"Normandie Universit\u00e9 , UR 4650, Physiopathologie et Strat\u00e9gies d'Imagerie du Remodelage cardiovasculaire, GIP Cyceron, Caen, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,6,19]]},"reference":[{"key":"2026010712094746900_bib1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2021.712354","article-title":"Species-specific effects of cation channel TRPM4 small-molecule inhibitors","volume":"12","author":"Arullampalam","year":"2021","journal-title":"Frontiers in Pharmacology"},{"key":"2026010712094746900_bib2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19051413","article-title":"Beta-estradiol regulates voltage-gated calcium channels and estrogen receptors in telocytes from human myometrium","volume":"19","author":"Banciu","year":"2018","journal-title":"International Journal of Molecular Sciences"},{"key":"2026010712094746900_bib3","doi-asserted-by":"publisher","first-page":"1148","DOI":"10.1038\/ni.1648","article-title":"The calcium-activated nonselective cation channel TRPM4 is essential for the migration but not the maturation of dendritic cells","volume":"9","author":"Barbet","year":"2008","journal-title":"Nature Immunology"},{"key":"2026010712094746900_bib4","doi-asserted-by":"publisher","first-page":"688.e1","DOI":"10.1016\/j.ajog.2014.06.018","article-title":"Calcium-activated chloride channels anoctamin 1 and 2 promote murine uterine smooth muscle contractility","volume":"211","author":"Bernstein","year":"2014","journal-title":"American Journal of Obstetrics and Gynecology"},{"key":"2026010712094746900_bib5","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1113\/jphysiol.2007.132126","article-title":"Characterization of the molecular and electrophysiological properties of the T-type calcium channel in human myometrium","volume":"581","author":"Blanks","year":"2007","journal-title":"Journal of Physiology"},{"key":"2026010712094746900_bib6","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1007\/BF00586350","article-title":"Responses to catecholamines of the rat isolated uterus throughout the natural oestrous cycle","volume":"321","author":"Boyle","year":"1982","journal-title":"Naunyn-Schmiedeberg\u2019s Archives of Pharmacology"},{"key":"2026010712094746900_bib7","doi-asserted-by":"publisher","first-page":"C832","DOI":"10.1152\/ajpcell.00268.2006","article-title":"Myometrial expression of small conductance Ca2+-activated K+ channels depresses phasic uterine contraction","volume":"292","author":"Brown","year":"2007","journal-title":"American Journal of Physiology. 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