{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T05:17:42Z","timestamp":1782364662054,"version":"3.54.5"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T00:00:00Z","timestamp":1695945600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2023,11,1]]},"abstract":"<jats:title>\u2003<\/jats:title>\n                  <jats:sec>\n                    <jats:title>In brief<\/jats:title>\n                    <jats:p>Autophagy is important for trophoblast cells at the maternal\u2013fetal interface during early pregnancy. This study suggests that trophoblast cells can promote the autophagy under a regulation of the LPA\/LPAR 1\u2013NHE1 axis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>The autophagy of trophoblasts is necessary for developing and maintaining a healthy pregnancy. Autophagy dysfunction in trophoblast cells is linked to recurrent spontaneous abortion (RSA). However, the mechanism underlying trophoblast autophagy is unknown. In this study, we investigated the expression of autophagy-related genes in both normal and RSA villi. We also examined the production of LPA and LPAR1 in trophoblast cells during early pregnancy. We found that the activation of the LPA\u2013LPAR1 axis triggered the autophagy of trophoblast cells and increased the expression of NHE1. Inhibition of NHE1 suppressed the autophagy in trophoblast cells and we confirmed that NHE1 regulates LPA production in trophoblast cells. Additionally, we found decreased expression of autophagy-related genes and LPAR1 in villi from RSA patients. These observations indicate that the LPA\/LPAR1\u2013NHE1 axis regulates the autophagy of trophoblast cells during pregnancy. Insufficient autophagy and poor expression of LPAR1 in trophoblast cells may result in the dysfunction of the trophoblasts and an increased risk of spontaneous abortion. Overall, our research elucidated that a positive LPA\/LPAR1\u2013NHE1 axis can promote the autophagy of trophoblast cells and the abnormal axis leads to the autophagy deficiency of trophoblast cells in recurrent spontaneous abortion.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/rep-23-0224","type":"journal-article","created":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T14:01:11Z","timestamp":1694527271000},"page":"357-368","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":9,"title":["An abnormal LPA\/LPAR1\u2013NHE1 axis leads to the autophagy deficiency of trophoblast cells in recurrent spontaneous abortion"],"prefix":"10.1093","volume":"166","author":[{"given":"Min","family":"Chen","sequence":"first","affiliation":[{"name":"Laboratory for Reproductive Immunology , Hospital of Obstetrics and Gynecology, Shanghai Medical School, Fudan University, Shanghai, People\u2019s Republic of 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