{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T23:42:24Z","timestamp":1784936544387,"version":"3.55.0"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2024,5,2]],"date-time":"2024-05-02T00:00:00Z","timestamp":1714608000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,6,1]]},"abstract":"<jats:title>\u2003<\/jats:title>\n                  <jats:sec>\n                    <jats:title>In brief<\/jats:title>\n                    <jats:p>The mechanism underlying the accumulation of \u03b3\u03b4T cells in the decidua, which helps maintain maternal\u2013fetal immunotolerance in early pregnancy, is unknown. This study reveals that DSC-derived RANKL upregulates ICAM-1 expression via the NF-\u03baB pathway to enable \u03b3\u03b4T cell accumulation in the early decidua.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>Decidual \u03b3\u03b4T (d\u03b3\u03b4T) cells help maintain maternal\u2013fetal immunotolerance in early pregnancy. However, the mechanism underlying the accumulation of \u03b3\u03b4T cells in the decidua is unknown. Previous work showed that RANKL upregulated intercellular adhesion molecule 1 (ICAM-1) in decidual stromal cells (DSCs), and Rankl knockout mice had limited d\u03b3\u03b4T cell populations. In this study, we measured the expression levels of RANKL\/RANK and ICAM-1 in DSCs, in addition to the integrins of ICAM-1 on d\u03b3\u03b4T cells, and the number of d\u03b3\u03b4T cells from patients with recurrent spontaneous abortion (RSA) and normal pregnant women in the first trimester. RSA patients showed significantly decreased RANKL\/RANK and ICAM-1\/CD11a signaling in decidua, and a decreased percentage of d\u03b3\u03b4T cells, which was positively correlated with DSC-derived RANKL and ICAM-1. Next, an in vitro adhesion experiment showed that the enhanced attraction of human DSCs to d\u03b3\u03b4T cells after RANKL overexpression was almost completely aborted by anti-ICAM-1. Furthermore, Rankl knockout mice showed a significant reduction in NF-\u03baB activity compared with wild-type controls. Finally, we applied a selective NF-\u03baB inhibitor named PDTC to validate the role of NF-\u03baB in RANKL-mediated ICAM-1 upregulation. Taken together, our data show that DSC-derived RANKL upregulates ICAM-1 expression via the NF-\u03baB pathway to enable \u03b3\u03b4T cell accumulation in the early decidua. A reduction in RANKL\/ICAM-1 signaling in DSCs may result in insufficient accumulation of \u03b3\u03b4T cells in decidua and, in turn, RSA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/rep-23-0262","type":"journal-article","created":{"date-parts":[[2024,4,10]],"date-time":"2024-04-10T13:56:51Z","timestamp":1712757411000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":1,"title":["Accumulation of \u03b3\u03b4T cells in the decidua is promoted by RANKL which upregulates ICAM-1 via NF-\u03baB"],"prefix":"10.1093","volume":"167","author":[{"given":"Rui-Qi","family":"Chang","sequence":"first","affiliation":[{"name":"The Center for Reproductive Medicine , Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, People\u2019s Republic of China"},{"name":"Joint International Research Lab for Reproduction and Development , Ministry of Education, Chongqing, People\u2019s Republic of China"},{"name":"Reproduction and Stem Cell Therapy Research Center of Chongqing , Chongqing, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing-Cong","family":"Dai","sequence":"additional","affiliation":[{"name":"The Center for Reproductive Medicine , Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Han","family":"Qiu","sequence":"additional","affiliation":[{"name":"The Center for Reproductive Medicine , Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Liang","sequence":"additional","affiliation":[{"name":"The Center for Reproductive Medicine , Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Yu","family":"Hu","sequence":"additional","affiliation":[{"name":"The Center for Reproductive Medicine , Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9276-0722","authenticated-orcid":false,"given":"Ming-Qing","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory for Reproductive Immunology , Hospital of Obstetrics and Gynecology, Fudan University, Shanghai, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2000-7894","authenticated-orcid":false,"given":"Fan","family":"He","sequence":"additional","affiliation":[{"name":"The Center for Reproductive Medicine , Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, People\u2019s Republic of China"},{"name":"Joint International Research Lab for Reproduction and Development , Ministry of Education, Chongqing, People\u2019s Republic of China"},{"name":"Reproduction and Stem Cell Therapy Research Center of Chongqing , Chongqing, People\u2019s Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,5,2]]},"reference":[{"key":"2026010712120422600_bib1","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/s41571-018-0095-y","article-title":"Roles of the RANKL-RANK axis in antitumour immunity - implications for therapy","volume":"15","author":"Ahern","year":"2018","journal-title":"Nature Reviews. 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