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This study suggests that an adipokine, FNDC3A, reduces IGF1-dependent glycolysis and may contribute to postpartum infertility.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>Dairy cows go through a period of subfertility after parturition, triggered in part by a disruption of energy homeostasis. The mobilization of body fat alters the secretion of adipokines, which have been shown to impact ovarian function. Fibronectin type III domain-containing 3A (FNDC3A) is a recently discovered adipokine-myokine, and FNDC3A mRNA abundance in subcutaneous adipose tissue is increased postpartum in cattle. In this study, we hypothesized that FNDC3A may compromise granulosa cell function in cattle and investigated this using a well-established in vitro cell culture model. Here, we demonstrate the presence of FNDC3A protein associated with extracellular vesicles in follicular fluid and in plasma, suggesting an endocrine role for this adipokine. FNDC3A protein and mRNA was also detected in the bovine ovary (cortex, granulosa and theca cells, cumulus, oocyte and corpus luteum). Abundance of FNDC3A mRNA in granulosa cells from small follicles was increased by in vitro treatment with the adipokines leptin and TNF but not by visfatin, resistin, adiponectin, chemerin or IGF1. Addition of recombinant FNDC3A at physiological doses (10 ng\/mL) to granulosa cells decreased IGF1-dependent progesterone but not estradiol secretion and IGF1-dependent lactate secretion and abundance of GLUT3 and GLUT4 mRNA. This concentration of FNDC3A increased cell viability, abundance of mRNA encoding a putative receptor FOLR1, and increased phosphorylation of Akt. Collectively, these data suggest that FNDC3A may regulate folliculogenesis in cattle by modulating IGF1-dependent granulosa cell steroidogenesis and glucose metabolism.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/rep-23-0451","type":"journal-article","created":{"date-parts":[[2024,3,18]],"date-time":"2024-03-18T13:55:15Z","timestamp":1710770115000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":4,"title":["Exosomal or follicular FNDC3A decreases FOLR1 mRNA abundance and progesterone and lactate synthesis in bovine granulosa cells"],"prefix":"10.1093","volume":"167","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9998-0421","authenticated-orcid":false,"given":"Mathilde","family":"Daudon","sequence":"first","affiliation":[{"name":"CNRS , IFCE, INRAE, Universit\u00e9 de Tours, PRC, Nouzilly, France"},{"name":"Centre de recherche en reproduction et fertilit\u00e9 , Facult\u00e9 de m\u00e9decine v\u00e9t\u00e9rinaire, Universit\u00e9 de Montr\u00e9al, Saint Hyacinthe, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Ram\u00e9","sequence":"additional","affiliation":[{"name":"CNRS , IFCE, INRAE, Universit\u00e9 de Tours, PRC, Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6727-8280","authenticated-orcid":false,"given":"Christopher A","family":"Price","sequence":"additional","affiliation":[{"name":"Centre de recherche en reproduction et fertilit\u00e9 , Facult\u00e9 de m\u00e9decine v\u00e9t\u00e9rinaire, Universit\u00e9 de Montr\u00e9al, Saint Hyacinthe, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8046-7705","authenticated-orcid":false,"given":"Jo\u00eblle","family":"Dupont","sequence":"additional","affiliation":[{"name":"CNRS , IFCE, INRAE, Universit\u00e9 de Tours, PRC, Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,4,17]]},"reference":[{"key":"2026010712105517700_bib1","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1677\/joe.0.1710339","article-title":"Decreased concentration of plasma leptin in periparturient dairy cows is caused by negative energy balance","volume":"171","author":"Block","year":"2001","journal-title":"Journal of Endocrinology"},{"key":"2026010712105517700_bib2","doi-asserted-by":"publisher","first-page":"1344","DOI":"10.1017\/S1751731113000475","article-title":"Selection of reference genes for quantitative real-time PCR normalisation in adipose tissue, muscle, liver and mammary gland from ruminants","volume":"7","author":"Bonnet","year":"2013","journal-title":"Animal"},{"key":"2026010712105517700_bib3","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1038\/nature10777","article-title":"A PGC1-\u03b1-dependent myokine that drives brown-fat-like development of white fat and thermogenesis","volume":"481","author":"Bostr\u00f6m","year":"2012","journal-title":"Nature"},{"key":"2026010712105517700_bib4","doi-asserted-by":"publisher","first-page":"e1700082","DOI":"10.1002\/prca.201700082","article-title":"Metabolic signature of microvesicles from umbilical cord mesenchymal stem cells of preterm and term infants","volume":"12","author":"Bruschi","year":"2018","journal-title":"Proteomics. 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