{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T23:17:45Z","timestamp":1767568665977,"version":"3.48.0"},"reference-count":64,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T00:00:00Z","timestamp":1747353600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,5,16]]},"abstract":"<jats:sec>\n                    <jats:title>In brief<\/jats:title>\n                    <jats:p>During uterine receptivity, exocytosis from uterine epithelial cells (UECs) contributes to uterine fluid composition, playing a role in communication with an implanting blastocyst. SNAREs, a family of proteins involved in exocytosis, are increased in the receptive uterine epithelium of the rat.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>Uterine luminal fluid is composed of secretions from the uterine luminal and glandular epithelial cells. The fluid composition plays a role in cell-to-cell communication between the receptive endometrium and an invading blastocyst. Part of this fluid is released from the epithelial cells via exocytosis, mechanisms regulating this are not yet understood. Using transmission electron microscopy, this study identified extracellular vesicles in the uterine lumen at the time of fertilisation and uterine receptivity. Immunofluorescence microscopy showed SNARE proteins syntaxin 2 and SNAP23 in the apical area of UECs at the time of receptivity. SNAP23 was also found in the uterine fluid on day 5.5 of early pregnancy. Western blotting of isolated UECs demonstrated a significant increase in syntaxin 2 and SNAP23 at the time of uterine receptivity compared to the time of fertilisation. The morphological evidence of extracellular vesicles in the uterine lumen and the presence of SNARE proteins syntaxin 2 and SNAP23 in the apical part of the luminal epithelium at the time of uterine receptivity suggests that exocytosis contributes to the composition of the uterine luminal fluid, a potential component of maternal\u2013foetal communication during early pregnancy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/rep-24-0188","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T16:20:02Z","timestamp":1747412402000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":0,"title":["Extracellular vesicles, syntaxin 2 and SNAP23 in the uterine microenvironment of the rat"],"prefix":"10.1093","volume":"169","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8178-6401","authenticated-orcid":true,"given":"Sadaf N","family":"Kalam","sequence":"first","affiliation":[{"name":"School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samson N","family":"Dowland","sequence":"additional","affiliation":[{"name":"School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Louise","family":"Cole","sequence":"additional","affiliation":[{"name":"Australian Institute for Microbiology and Infection, The Ithree Institute, Faculty of Science, University of Technology Sydney, Sydney, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura A","family":"Lindsay","sequence":"additional","affiliation":[{"name":"School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher R","family":"Murphy","sequence":"additional","affiliation":[{"name":"School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","reference":[{"key":"bib1","series-title":"Biochemistry","first-page":"3499","article-title":"Syntaxin 2 and SNAP-23 are required for regulated surfactant secretion","volume":"43","author":"Abonyo BO","year":"2004","unstructured":"Abonyo BO\n, \nGou D\n, \nWang P\n, et al.\n 2004 Syntaxin 2 and SNAP-23 are required for regulated surfactant secretion. Biochemistry 43 3499\u20133506. (https:\/\/doi.org\/10.1021\/bi036338y)"},{"key":"bib2","series-title":"Semin Cell Dev Biol","first-page":"204","article-title":"Uterine receptivity to human embryonic implantation: histology, biomarkers, and transcriptomics","volume":"19","author":"Aghajanova L","year":"2008","unstructured":"Aghajanova L\n, \nHamilton AE\n & \nGiudice LC\n\n 2008 Uterine receptivity to human embryonic implantation: histology, biomarkers, and transcriptomics. Semin Cell Dev Biol 19 204\u2013211. (https:\/\/doi.org\/10.1016\/j.semcdb.2007.10.008)"},{"key":"bib3","series-title":"Sci Rep","article-title":"Meta-signature of human endometrial receptivity: a meta-analysis and validation study of transcriptomic biomarkers","volume":"7","author":"Altm\u00e4e S","year":"2017","unstructured":"Altm\u00e4e S\n, \nKoel M\n, \nV\u00f5sa U\n, et al.\n 2017 Meta-signature of human endometrial receptivity: a meta-analysis and validation study of transcriptomic biomarkers. Sci Rep 7 10077. (https:\/\/doi.org\/10.1038\/s41598-017-10098-3)"},{"key":"bib4","series-title":"Placenta","first-page":"269","article-title":"The cell biology of human implantation","volume":"17","author":"Aplin JD","year":"1996","unstructured":"Aplin JD\n\n 1996 The cell biology of human implantation. Placenta 17 269\u2013275. (https:\/\/doi.org\/10.1016\/s0143-4004(96)90050-8)"},{"key":"bib5","series-title":"Rev Reprod","first-page":"84","article-title":"Adhesion molecules in implantation","volume":"2","author":"Aplin JD","year":"1997","unstructured":"Aplin JD\n\n 1997 Adhesion molecules in implantation. Rev Reprod 2 84\u201393. (https:\/\/doi.org\/10.1530\/ror.0.0020084)"},{"key":"bib6","series-title":"PLoS One","article-title":"Extracellular vesicles in luminal fluid of the ovine uterus","volume":"9","author":"Burns G","year":"2014","unstructured":"Burns G\n, \nBrooks K\n, \nWildung M\n, et al.\n 2014 Extracellular vesicles in luminal fluid of the ovine uterus. PLoS One 9 e90913. (https:\/\/doi.org\/10.1371\/journal.pone.0090913)"},{"key":"bib7","series-title":"Dev Biol","first-page":"217","article-title":"Embryo implantation","volume":"223","author":"Carson DD","year":"2000","unstructured":"Carson DD\n, \nBagchi I\n, \nDey SK\n, et al.\n 2000 Embryo implantation. Dev Biol 223 217\u2013237. (https:\/\/doi.org\/10.1006\/dbio.2000.9767)"},{"key":"bib8","series-title":"Brain Res","first-page":"11","article-title":"Organization of the secretory machinery in the rodent brain: distribution of the t-SNAREs, SNAP-25 and SNAP-23","volume":"831","author":"Chen D","year":"1999","unstructured":"Chen D\n, \nMinger SL\n, \nHoner WG\n, et al.\n 1999 Organization of the secretory machinery in the rodent brain: distribution of the t-SNAREs, SNAP-25 and SNAP-23. Brain Res 831 11\u201324. (https:\/\/doi.org\/10.1016\/s0006-8993(99)01371-2)"},{"key":"bib9","series-title":"Blood","first-page":"921","article-title":"Molecular mechanisms of platelet exocytosis: role of SNAP-23 and syntaxin 2 in dense core granule release","volume":"95","author":"Chen D","year":"2000","unstructured":"Chen D\n, \nBernstein AM\n, \nLemons PP\n, et al.\n 2000 Molecular mechanisms of platelet exocytosis: role of SNAP-23 and syntaxin 2 in dense core granule release. Blood 95 921\u2013929. (https:\/\/doi.org\/10.1182\/blood.v95.3.921.003k17_921_929)"},{"key":"bib10","series-title":"Am J Respir Cell Mol Biol","first-page":"677","article-title":"Effect of cholesterol depletion on exocytosis of alveolar type II cells","volume":"34","author":"Chintagari NR","year":"2006","unstructured":"Chintagari NR\n, \nJin N\n, \nWang P\n, et al.\n 2006 Effect of cholesterol depletion on exocytosis of alveolar type II cells. Am J Respir Cell Mol Biol 34 677\u2013687. (https:\/\/doi.org\/10.1165\/rcmb.2005-0418oc)"},{"key":"bib11","series-title":"Eur J Cell Biol","article-title":"Extracellular vesicles: mediators of embryo-maternal crosstalk during pregnancy and a new weapon to fight against infertility","volume":"99","author":"Das M","year":"2020","unstructured":"Das M\n & \nKale V\n\n 2020 Extracellular vesicles: mediators of embryo-maternal crosstalk during pregnancy and a new weapon to fight against infertility. Eur J Cell Biol 99 151125. (https:\/\/doi.org\/10.1016\/j.ejcb.2020.151125)"},{"key":"bib12","series-title":"Biochim Biophys Acta","first-page":"175","article-title":"Actin remodeling to facilitate membrane fusion","volume":"1641","author":"Eitzen G","year":"2003","unstructured":"Eitzen G\n\n 2003 Actin remodeling to facilitate membrane fusion. Biochim Biophys Acta 1641 175\u2013181. (https:\/\/doi.org\/10.1016\/s0167-4889(03)00087-9)"},{"key":"bib13","series-title":"J Lipid Res","first-page":"2185","article-title":"Megalin and cubilin expression in gallbladder epithelium and regulation by bile acids","volume":"45","author":"Erranz B","year":"2004","unstructured":"Erranz B\n, \nMiquel JF\n, \nArgraves WS\n, et al.\n 2004 Megalin and cubilin expression in gallbladder epithelium and regulation by bile acids. J Lipid Res 45 2185\u20132198. (https:\/\/doi.org\/10.1194\/jlr.m400235-jlr200)"},{"key":"bib14","series-title":"Assisted Reproductive Technology in Australia and New Zealand 2016","author":"Fitzgerald O","year":"2018","unstructured":"Fitzgerald O\n, \nPaul RC\n, \nHarris K\n, et al\n. 2018 Assisted Reproductive Technology in Australia and New Zealand 2016. Sydney: National Perinatal Epidemiology and Statistics Unit, the University of New South Wales. (https:\/\/www.unsw.edu.au\/content\/dam\/pdfs\/medicine-health\/npesu\/research-reports\/2023-12-npesu\/2024-01-Assisted-Reproductive-Technology-in-Australia-and-New-Zealand-2016.pdf)"},{"key":"bib15","series-title":"Mol Hum Reprod","first-page":"833","article-title":"Uterosome-like vesicles prompt human sperm fertilizing capability","volume":"22","author":"Franchi A","year":"2016","unstructured":"Franchi A\n, \nCubilla M\n, \nGuidobaldi HA\n, et al.\n 2016 Uterosome-like vesicles prompt human sperm fertilizing capability. Mol Hum Reprod 22 833\u2013841. (https:\/\/doi.org\/10.1093\/molehr\/gaw066)"},{"key":"bib16","series-title":"Environ Health Perspect","first-page":"129","article-title":"Biological markers during early pregnancy: trophoblastic signals of the peri-implantation period","volume":"74","author":"Glasser SR","year":"1987","unstructured":"Glasser SR\n, \nJulian J\n, \nMunir MI\n, et al.\n 1987 Biological markers during early pregnancy: trophoblastic signals of the peri-implantation period. Environ Health Perspect 74 129\u2013147. (https:\/\/doi.org\/10.2307\/3430443)"},{"key":"bib17","series-title":"Mol Reprod Dev","first-page":"1627","article-title":"Investigating the role of murine epididymosomes and uterosomes in GPI-linked protein transfer to sperm using SPAM1 as a model","volume":"75","author":"Griffiths GS","year":"2008","unstructured":"Griffiths GS\n, \nGalileo DS\n, \nReese K\n, et al.\n 2008 Investigating the role of murine epididymosomes and uterosomes in GPI-linked protein transfer to sperm using SPAM1 as a model. Mol Reprod Dev 75 1627\u20131636. (https:\/\/doi.org\/10.1002\/mrd.20907)"},{"key":"bib18","series-title":"Reprod Biol","article-title":"The role of extracellular vesicles in endometrial receptivity and their potential in reproductive therapeutics and diagnosis","volume":"22","author":"Hart AR","year":"2022","unstructured":"Hart AR\n, \nKhan NLA\n, \nGodakumara K\n, et al.\n 2022 The role of extracellular vesicles in endometrial receptivity and their potential in reproductive therapeutics and diagnosis. Reprod Biol 22 100645. (https:\/\/doi.org\/10.1016\/j.repbio.2022.100645)"},{"key":"bib19","series-title":"Biochim Biophys Acta","first-page":"120","article-title":"SNAREs and traffic","volume":"1744","author":"Hong W","year":"2005","unstructured":"Hong W\n\n 2005 SNAREs and traffic. Biochim Biophys Acta 1744 120\u2013144. (https:\/\/doi.org\/10.1016\/j.bbamcr.2005.03.014)"},{"key":"bib20","series-title":"J Nanobiotechnology","article-title":"Uterine luminal-derived extracellular vesicles: potential nanomaterials to improve embryo implantation","volume":"21","author":"Hong L","year":"2023","unstructured":"Hong L\n, \nZang X\n, \nHu Q\n, et al.\n 2023 Uterine luminal-derived extracellular vesicles: potential nanomaterials to improve embryo implantation. J Nanobiotechnology 21 79. (https:\/\/doi.org\/10.1186\/s12951-023-01834-1)"},{"key":"bib21","series-title":"Nature","first-page":"646","article-title":"Vesicular restriction of synaptobrevin suggests a role for calcium in membrane fusion","volume":"415","author":"Hu K","year":"2002","unstructured":"Hu K\n, \nCarroll J\n, \nFedorovich S\n, et al.\n 2002 Vesicular restriction of synaptobrevin suggests a role for calcium in membrane fusion. Nature 415 646\u2013650. (https:\/\/doi.org\/10.1038\/415646a)"},{"key":"bib22","series-title":"Reprod Sci","first-page":"1683","article-title":"Ovarian stimulation altered uterine fluid extracellular vesicles miRNA affecting implantation in rats","volume":"31","author":"Huang X","year":"2024","unstructured":"Huang X\n, \nZhao J\n, \nZhang Q\n, et al.\n 2024 Ovarian stimulation altered uterine fluid extracellular vesicles miRNA affecting implantation in rats. Reprod Sci 31 1683\u20131694. (https:\/\/doi.org\/10.1007\/s43032-023-01448-w)"},{"key":"bib23","series-title":"Arch Oral Biol","first-page":"597","article-title":"Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane","volume":"48","author":"Imai A","year":"2003","unstructured":"Imai A\n, \nNashida T\n, \nYoshie S\n, et al.\n 2003 Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane. Arch Oral Biol 48 597\u2013604. (https:\/\/doi.org\/10.1016\/s0003-9969(03)00116-x)"},{"key":"bib24","series-title":"Nat Rev Mol Cell Biol","first-page":"631","article-title":"SNAREs \u2013 engines for membrane fusion","volume":"7","author":"Jahn R","year":"2006","unstructured":"Jahn R\n & \nScheller RH\n\n 2006 SNAREs \u2013 engines for membrane fusion. Nat Rev Mol Cell Biol 7 631\u2013643. (https:\/\/doi.org\/10.1038\/nrm2002)"},{"key":"bib25","series-title":"Cell Tissue Res","first-page":"1","article-title":"Microtubules are reorganised and fragmented for uterine receptivity","volume":"374","author":"Kalam SN","year":"2018","unstructured":"Kalam SN\n, \nDowland S\n, \nLindsay L\n, et al.\n 2018 Microtubules are reorganised and fragmented for uterine receptivity. Cell Tissue Res 374 1\u201311. (https:\/\/doi.org\/10.1007\/s00441-018-2887-x)"},{"key":"bib26","series-title":"Reproduction","first-page":"533","article-title":"Membrane trafficking directed by VAMP2 and syntaxin 3 in uterine epithelial cells","volume":"160","author":"Kalam SN","year":"2020","unstructured":"Kalam SN\n, \nCole L\n, \nLindsay L\n, et al.\n 2020 Membrane trafficking directed by VAMP2 and syntaxin 3 in uterine epithelial cells. Reproduction 160 533\u2013546. (https:\/\/doi.org\/10.1530\/REP-20-0233)"},{"key":"bib27","series-title":"J Morphol","first-page":"1381","article-title":"Porosomes in uterine epithelial cells: ultrastructural identification and characterisation during early pregnancy","volume":"283","author":"Kalam SN","year":"2022","unstructured":"Kalam SN\n, \nDowland S\n, \nLindsay L\n, et al.\n 2022 Porosomes in uterine epithelial cells: ultrastructural identification and characterisation during early pregnancy. J Morphol 283 1381\u20131389. (https:\/\/doi.org\/10.1002\/jmor.21504)"},{"key":"bib28","series-title":"Reprod Fertil Dev","first-page":"892","article-title":"Focal adhesions disassemble during early pregnancy in rat uterine epithelial cells","volume":"20","author":"Kaneko Y","year":"2008","unstructured":"Kaneko Y\n, \nLindsay LA\n & \nMurphy CR\n\n 2008 Focal adhesions disassemble during early pregnancy in rat uterine epithelial cells. Reprod Fertil Dev 20 892\u2013899. (https:\/\/doi.org\/10.1071\/rd08148)"},{"key":"bib29","series-title":"Sci Rep","first-page":"1","article-title":"Uterine glands impact uterine receptivity, luminal fluid homeostasis and blastocyst implantation","volume":"6","author":"Kelleher AM","year":"2016","unstructured":"Kelleher AM\n, \nBurns GW\n, \nBehura S\n, et al.\n 2016 Uterine glands impact uterine receptivity, luminal fluid homeostasis and blastocyst implantation. Sci Rep 6 1\u201318. (https:\/\/doi.org\/10.1038\/srep38078)"},{"key":"bib30","series-title":"Biophys J","first-page":"2863","article-title":"Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells","volume":"78","author":"Lang T","year":"2000","unstructured":"Lang T\n, \nWacker I\n, \nWunderlich I\n, et al.\n 2000 Role of actin cortex in the subplasmalemmal transport of secretory granules in PC-12 cells. Biophys J 78 2863\u20132877. (https:\/\/doi.org\/10.1016\/s0006-3495(00)76828-7)"},{"key":"bib31","series-title":"Baillieres Best Pract Res Clin Obstet Gynaecol","first-page":"775","article-title":"Endometrial receptivity and the window of implantation","volume":"14","author":"Lessey BA","year":"2000","unstructured":"Lessey BA\n\n 2000 Endometrial receptivity and the window of implantation. Baillieres Best Pract Res Clin Obstet Gynaecol 14 775\u2013788. (https:\/\/doi.org\/10.1053\/beog.2000.0118)"},{"key":"bib32","series-title":"Reprod Biol Endocrinol","first-page":"90","article-title":"Role of microRNAs in embryo implantation","volume":"15","author":"Liang J","year":"2017","unstructured":"Liang J\n, \nWang S\n & \nWang Z\n\n 2017 Role of microRNAs in embryo implantation. Reprod Biol Endocrinol 15 90. (https:\/\/doi.org\/10.1186\/s12958-017-0309-7)"},{"key":"bib33","series-title":"Reprod Fertil Dev","first-page":"982","article-title":"Ovarian hyperstimulation affects fluid transporters in the uterus: a potential mechanism in uterine receptivity","volume":"26","author":"Lindsay LA","year":"2014","unstructured":"Lindsay LA\n & \nMurphy CR\n\n 2014 Ovarian hyperstimulation affects fluid transporters in the uterus: a potential mechanism in uterine receptivity. Reprod Fertil Dev 26 982\u2013990. (https:\/\/doi.org\/10.1071\/rd12396)"},{"key":"bib34","series-title":"Reproduction","first-page":"753","article-title":"Uterine focal adhesions are retained at implantation after rat ovarian hyperstimulation","volume":"152","author":"Lindsay LA","year":"2016","unstructured":"Lindsay LA\n, \nDowland SN\n & \nMurphy CR\n\n 2016 Uterine focal adhesions are retained at implantation after rat ovarian hyperstimulation. Reproduction 152 753\u2013763. (https:\/\/doi.org\/10.1530\/rep-16-0331)"},{"key":"bib35","series-title":"Mol Cell Proteomics","article-title":"Quantitative proteomics Identifies proteins enriched in large and small extracellular vesicles","volume":"21","author":"Lischnig A","year":"2022","unstructured":"Lischnig A\n, \nBergqvist M\n, \nOchiya T\n, et al.\n 2022 Quantitative proteomics Identifies proteins enriched in large and small extracellular vesicles. Mol Cell Proteomics 21 100273. (https:\/\/doi.org\/10.1016\/j.mcpro.2022.100273)"},{"key":"bib36","series-title":"J Biol Chem","first-page":"3422","article-title":"Targeting of SNAP-23 and SNAP-25 in polarized epithelial cells","volume":"273","author":"Low SH","year":"1998","unstructured":"Low SH\n, \nRoche PA\n, \nAnderson HA\n, et al.\n 1998 Targeting of SNAP-23 and SNAP-25 in polarized epithelial cells. J Biol Chem 273 3422\u20133430. (https:\/\/doi.org\/10.1074\/jbc.273.6.3422)"},{"key":"bib37","series-title":"Micron","first-page":"61","article-title":"Correlated light and electron microscopy observations of the uterine epithelial cell actin cytoskeleton using fluorescently labeled resin-embedded sections","volume":"84","author":"Moore CL","year":"2016","unstructured":"Moore CL\n, \nCheng D\n, \nShami GJ\n, et al.\n 2016 Correlated light and electron microscopy observations of the uterine epithelial cell actin cytoskeleton using fluorescently labeled resin-embedded sections. Micron 84 61\u201366. (https:\/\/doi.org\/10.1016\/j.micron.2016.02.010)"},{"key":"bib38","series-title":"Prog Histochem Cytochem","first-page":"1","article-title":"The plasma membrane of uterine epithelial cells: structure and histochemistry","volume":"27","author":"Murphy CR","year":"1993","unstructured":"Murphy CR\n\n 1993 The plasma membrane of uterine epithelial cells: structure and histochemistry. Prog Histochem Cytochem 27 1\u201366. (https:\/\/doi.org\/10.1016\/s0079-6336(11)80004-5)"},{"key":"bib39","series-title":"Cell Biol Int","first-page":"1115","article-title":"Plasma membrane transformation: a common response of uterine epithelial cells during the peri-implantation period","volume":"18","author":"Murphy CR","year":"1994","unstructured":"Murphy CR\n\n 1994 Plasma membrane transformation: a common response of uterine epithelial cells during the peri-implantation period. Cell Biol Int 18 1115\u20131128. (https:\/\/doi.org\/10.1006\/cbir.1994.1038)"},{"key":"bib40","series-title":"Cell Res","first-page":"259","article-title":"Uterine receptivity and the plasma membrane transformation","volume":"14","author":"Murphy CR","year":"2004","unstructured":"Murphy CR\n\n 2004 Uterine receptivity and the plasma membrane transformation. Cell Res 14 259\u2013267. (https:\/\/doi.org\/10.1038\/sj.cr.7290227)"},{"key":"bib42","series-title":"J Cell Sci","first-page":"163","article-title":"Cholesterol in the plasma membrane of uterine epithelial cells: a freeze-fracture cytochemical study with digitonin","volume":"78","author":"Murphy CR","year":"1985","unstructured":"Murphy CR\n & \nMartin B\n\n 1985 Cholesterol in the plasma membrane of uterine epithelial cells: a freeze-fracture cytochemical study with digitonin. J Cell Sci 78 163\u2013172. (https:\/\/doi.org\/10.1242\/jcs.78.1.163)"},{"key":"bib41","series-title":"Acta Anat","first-page":"76","article-title":"Increase in cholesterol in the apical plasma membrane of uterine epithelial cells during early pregnancy in the rat","volume":"128","author":"Murphy CR","year":"1987","unstructured":"Murphy CR\n & \nDwarte DM\n\n 1987 Increase in cholesterol in the apical plasma membrane of uterine epithelial cells during early pregnancy in the rat. Acta Anat 128 76\u201379. (https:\/\/doi.org\/10.1159\/000146319)"},{"key":"bib43","series-title":"Ochsner J","first-page":"439","article-title":"Circulating placental extracellular vesicles and their potential roles during pregnancy","volume":"20","author":"Nakahara A","year":"2020","unstructured":"Nakahara A\n, \nNair S\n, \nOrmazabal V\n, et al.\n 2020 Circulating placental extracellular vesicles and their potential roles during pregnancy. Ochsner J 20 439. (https:\/\/doi.org\/10.31486\/toj.20.0049)"},{"key":"bib44","series-title":"Sci Rep","article-title":"Uterine epithelial Gp130 orchestrates hormone response and epithelial remodeling for successful embryo attachment in mice","volume":"13","author":"Namiki T","year":"2023","unstructured":"Namiki T\n, \nTerakawa J\n, \nKarakama H\n, et al.\n 2023 Uterine epithelial Gp130 orchestrates hormone response and epithelial remodeling for successful embryo attachment in mice. Sci Rep 13 854. (https:\/\/doi.org\/10.1038\/s41598-023-27859-y)"},{"key":"bib45","series-title":"PLoS One","article-title":"Endometrial exosomes\/microvesicles in the uterine microenvironment: a new paradigm for embryo-endometrial cross talk at implantation","volume":"8","author":"Ng YH","year":"2013","unstructured":"Ng YH\n, \nRome S\n, \nJalabert A\n, et al.\n 2013 Endometrial exosomes\/microvesicles in the uterine microenvironment: a new paradigm for embryo-endometrial cross talk at implantation. PLoS One 8 e58502. (https:\/\/doi.org\/10.1371\/journal.pone.0058502)"},{"key":"bib46","series-title":"Biol Reprod","first-page":"109","article-title":"Extracellular vesicles in the intrauterine environment: challenges and potential functions","volume":"95","author":"Nguyen HPT","year":"2016","unstructured":"Nguyen HPT\n, \nSimpson RJ\n, \nSalamonsen LA\n, et al.\n 2016 Extracellular vesicles in the intrauterine environment: challenges and potential functions. Biol Reprod 95 109. (https:\/\/doi.org\/10.1095\/biolreprod.116.143503)"},{"key":"bib47","series-title":"Acta Histochem","first-page":"42","article-title":"Ovarian hormones control the changing expression of claudins and occludin in rat uterine epithelial cells during early pregnancy","volume":"112","author":"Nicholson MDO","year":"2010","unstructured":"Nicholson MDO\n, \nLindsay LA\n & \nMurphy CR\n\n 2010 Ovarian hormones control the changing expression of claudins and occludin in rat uterine epithelial cells during early pregnancy. Acta Histochem 112 42\u201352. (https:\/\/doi.org\/10.1016\/j.acthis.2008.07.003)"},{"key":"bib48","series-title":"Biol Reprod","first-page":"915","article-title":"Apical vesicles in the rat uterine epithelium during early pregnancy: a morphometric study","volume":"26","author":"Parr MM","year":"1982","unstructured":"Parr MM\n\n 1982 Apical vesicles in the rat uterine epithelium during early pregnancy: a morphometric study. Biol Reprod 26 915\u2013924. (https:\/\/doi.org\/10.1095\/biolreprod26.5.915)"},{"key":"bib49","series-title":"Placenta","first-page":"30","article-title":"Extracellular vesicles-crucial players in human pregnancy","volume":"140","author":"Paul N","year":"2023","unstructured":"Paul N\n, \nSultana Z\n, \nFisher JJ\n, et al.\n 2023 Extracellular vesicles-crucial players in human pregnancy. Placenta 140 30\u201338. (https:\/\/doi.org\/10.1016\/j.placenta.2023.07.006)"},{"key":"bib50","series-title":"Cell Motil Cytoskeleton","first-page":"37","article-title":"Effects of latrunculin reveal requirements for the actin cytoskeleton during secretion from mast cells","volume":"48","author":"Pendleton A","year":"2001","unstructured":"Pendleton A\n & \nKoffer A\n\n 2001 Effects of latrunculin reveal requirements for the actin cytoskeleton during secretion from mast cells. Cell Motil Cytoskeleton 48 37\u201351. (https:\/\/doi.org\/10.1002\/1097-0169(200101)48:1<37::aid-cm4>3.0.co;2-0)"},{"key":"bib51","series-title":"J Biol Chem","first-page":"1506","article-title":"The plasma membrane Q-SNARE syntaxin 2 enters the zymogen granule membrane during exocytosis in the pancreatic acinar cell","volume":"280","author":"Pickett JA","year":"2005","unstructured":"Pickett JA\n, \nThorn P\n & \nEdwardson JM\n\n 2005 The plasma membrane Q-SNARE syntaxin 2 enters the zymogen granule membrane during exocytosis in the pancreatic acinar cell. J Biol Chem 280 1506\u20131511. (https:\/\/doi.org\/10.1074\/jbc.m411967200)"},{"key":"bib52","series-title":"Mol Cell Proteomics","article-title":"Uterine fluid extracellular vesicles proteome is altered during the estrous cycle","volume":"22","author":"Piibor J","year":"2023","unstructured":"Piibor J\n, \nWaldmann A\n, \nDissanayake K\n, et al.\n 2023 Uterine fluid extracellular vesicles proteome is altered during the estrous cycle. Mol Cell Proteomics 22 100642. (https:\/\/doi.org\/10.1016\/j.mcpro.2023.100642)"},{"key":"bib53","series-title":"Am J Reprod Immunol","first-page":"218","article-title":"The microenvironment of human implantation: determinant of reproductive success","volume":"75","author":"Salamonsen LA","year":"2016","unstructured":"Salamonsen LA\n, \nEvans J\n, \nNguyen HPT\n, et al.\n 2016 The microenvironment of human implantation: determinant of reproductive success. Am J Reprod Immunol 75 218\u2013225. (https:\/\/doi.org\/10.1111\/aji.12450)"},{"key":"bib54","series-title":"J Membr Biol","first-page":"17","article-title":"The hormonal control of uterine luminal fluid secretion and absorption","volume":"206","author":"Salleh N","year":"2005","unstructured":"Salleh N\n, \nBaines DL\n, \nNaftalin RJ\n, et al.\n 2005 The hormonal control of uterine luminal fluid secretion and absorption. J Membr Biol 206 17\u201328. (https:\/\/doi.org\/10.1007\/s00232-005-0770-7)"},{"key":"bib55","series-title":"J Anat","first-page":"3","article-title":"Adhesion molecules in endometrial epithelium: tissue integrity and embryo implantation","volume":"215","author":"Singh H","year":"2009","unstructured":"Singh H\n & \nAplin JD\n\n 2009 Adhesion molecules in endometrial epithelium: tissue integrity and embryo implantation. J Anat 215 3\u201313. (https:\/\/doi.org\/10.1111\/j.1469-7580.2008.01034.x)"},{"key":"bib56","series-title":"Nature","first-page":"318","article-title":"SNAP receptors implicated in vesicle targeting and fusion","volume":"362","author":"S\u00f6llner T","year":"1993","unstructured":"S\u00f6llner T\n, \nWhiteheart SW\n, \nBrunner M\n, et al.\n 1993 SNAP receptors implicated in vesicle targeting and fusion. Nature 362 318\u2013324. (https:\/\/doi.org\/10.1038\/362318a0)"},{"key":"bib57","series-title":"Eur J Med Res","article-title":"Current knowledge on the role of extracellular vesicles in endometrial receptivity","volume":"28","author":"Sui C","year":"2023","unstructured":"Sui C\n, \nLiao Z\n, \nBai J\n, et al.\n 2023 Current knowledge on the role of extracellular vesicles in endometrial receptivity. Eur J Med Res 28 471. (https:\/\/doi.org\/10.1186\/s40001-023-01459-y)"},{"key":"bib58","series-title":"Nature","first-page":"347","article-title":"Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 a resolution","volume":"395","author":"Sutton RB","year":"1998","unstructured":"Sutton RB\n, \nFasshauer D\n, \nJahn R\n, et al.\n 1998 Crystal structure of a SNARE complex involved in synaptic exocytosis at 2.4 a resolution. Nature 395 347\u2013353. (https:\/\/doi.org\/10.1038\/26412)"},{"key":"bib59","series-title":"Cells","first-page":"645","article-title":"MicroRNAs in small extracellular vesicles indicate successful embryo implantation during early pregnancy","volume":"9","author":"Tan Q","year":"2020","unstructured":"Tan Q\n, \nShi S\n, \nLiang J\n, et al.\n 2020 MicroRNAs in small extracellular vesicles indicate successful embryo implantation during early pregnancy. Cells 9 645. (https:\/\/doi.org\/10.3390\/cells9030645)"},{"key":"bib60","series-title":"Cell Mol Immunol","first-page":"548","article-title":"Extracellular vesicles and reproduction\u2013promotion of successful pregnancy","volume":"11","author":"Tannetta D","year":"2014","unstructured":"Tannetta D\n, \nDragovic R\n, \nAlyahyaei Z\n, et al.\n 2014 Extracellular vesicles and reproduction\u2013promotion of successful pregnancy. Cell Mol Immunol 11 548\u2013563. (https:\/\/doi.org\/10.1038\/cmi.2014.42)"},{"key":"bib61","series-title":"J Extracell Vesicles","article-title":"Minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches","volume":"13","author":"Welsh JA","year":"2024","unstructured":"Welsh JA\n, \nGoberdhan DCI\n, \nO\u2019Driscoll L\n, et al.\n 2024 Minimal information for studies of extracellular vesicles (MISEV2023): from basic to advanced approaches. J Extracell Vesicles 13 e12404. (https:\/\/doi.org\/10.1002\/jev2.12404)"},{"key":"bib62","series-title":"Trends Mol Med","first-page":"604","article-title":"Uterine fluid in pregnancy: a biological and clinical outlook","volume":"23","author":"Zhang Y","year":"2017","unstructured":"Zhang Y\n, \nWang Q\n, \nWang H\n, et al.\n 2017 Uterine fluid in pregnancy: a biological and clinical outlook. Trends Mol Med 23 604\u2013614. (https:\/\/doi.org\/10.1016\/j.molmed.2017.05.002)"},{"key":"bib63","series-title":"Proc Natl Acad Sci U S A","article-title":"VAMP3 and SNAP23 mediate the disturbed flow-induced endothelial microRNA secretion and smooth muscle hyperplasia","volume":"114","author":"Zhu J-J","year":"2017","unstructured":"Zhu J-J\n, \nLiu Y-F\n, \nZhang Y-P\n, et al.\n 2017 VAMP3 and SNAP23 mediate the disturbed flow-induced endothelial microRNA secretion and smooth muscle hyperplasia. Proc Natl Acad Sci U S A 114 201700561. (https:\/\/doi.org\/10.1073\/pnas.1700561114)"},{"key":"bib64","series-title":"Sci Rep","first-page":"1","article-title":"Extracellular vesicles are dynamic regulators of maternal glucose homeostasis during pregnancy","volume":"13","author":"Zierden HC","year":"2023","unstructured":"Zierden HC\n, \nMarx-Rattner R\n, \nRock KD\n, et al.\n 2023 Extracellular vesicles are dynamic regulators of maternal glucose homeostasis during pregnancy. Sci Rep 13 1\u201314. 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