{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T20:36:43Z","timestamp":1783111003857,"version":"3.54.6"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"4","funder":[{"name":"National Key Research and Development Program of China","award":["2021YFA0910103"],"award-info":[{"award-number":["2021YFA0910103"]}]},{"name":"Changsha Natural Science Foundation","award":["kq2402085"],"award-info":[{"award-number":["kq2402085"]}]},{"name":"The open research fund of the Hunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control","award":["HPKL2023035; HPKL2023002"],"award-info":[{"award-number":["HPKL2023035; HPKL2023002"]}]}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,2,4]]},"abstract":"<jats:sec>\n                    <jats:title>In brief<\/jats:title>\n                    <jats:p>\n                      The dysregulation of spermatogonial proliferation is one of the causes of male infertility. Using mice as an animal model, this study reveals the function and mechanism of m\n                      <jats:sup>6<\/jats:sup>\n                      A methylation reader insulin-like growth factor-2 mRNA-binding protein 1 (IGF2BP1) and its target\n                      <jats:italic>Akt2<\/jats:italic>\n                      in spermatogonia.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      IGF2BP1, as a newly identified N6-methyladenosine (m\n                      <jats:sup>6<\/jats:sup>\n                      A) methylation reader, plays a key role in mRNA stability, alternative splicing and translation. However, the function of IGF2BP1 and its target genes in the development of male germ cells remains unclear. In the present study, Western blotting, immunofluorescence and other cellular methods were used to confirm that IGF2BP1 is expressed throughout the male germline with high abundance in spermatogonia of mice, and that its downregulation inhibits spermatogonia proliferation\n                      <jats:italic>in vitro<\/jats:italic>\n                      . Through RNA sequencing, dual luciferase reporter assays, RIP-qPCR and mRNA stability experiments, it was identified that\n                      <jats:italic>Akt2<\/jats:italic>\n                      is one of the target genes of IGF2BP1. By stabilizing\n                      <jats:italic>Akt2<\/jats:italic>\n                      mRNA in an m\n                      <jats:sup>6<\/jats:sup>\n                      A-dependent manner, IGF2BP1 maintains spermatogonial proliferation. Furthermore, co-immunoprecipitation and mass spectrometry analyses revealed that PABPC1, DDX5 and FXR1 interact with IGF2BP1, and that the interaction between IGF2BP1 and PABPC1 promotes AKT2 expression. Finally, following the knockdown of both IGF2BP1 and AKT2, glucose and ATP levels in C18-4 and GC-1 spermatogonia cells were found to be reduced, indicating that IGF2BP1 regulates glucose metabolism homeostasis by stabilizing\n                      <jats:italic>Akt2<\/jats:italic>\n                      expression, thereby influencing spermatogonia proliferation. In addition, analysis of the cryptorchidism database from NCBI revealed that the expression of\n                      <jats:italic>IGF2BP1<\/jats:italic>\n                      and\n                      <jats:italic>AKT2<\/jats:italic>\n                      is significantly downregulated in cryptorchid patients with oligospermia or azoospermia. In conclusion, our study elucidates the role and underlying mechanism of IGF2BP1 in spermatogonia, providing a candidate target for male infertility.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/rep-24-0202","type":"journal-article","created":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T07:55:14Z","timestamp":1738655714000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":4,"title":["IGF2BP1 stabilizes Akt2 mRNA to promote glucose metabolism and maintain spermatogonial proliferation"],"prefix":"10.1093","volume":"169","author":[{"given":"Yanan","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Life Science, College of Biology, Hunan University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Life Science, College of Biology, Hunan University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiahui","family":"Fan","sequence":"additional","affiliation":[{"name":"Department of Life Science, College of Biology, Hunan University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziqian","family":"Min","sequence":"additional","affiliation":[{"name":"Department of Life Science, College of Biology, Hunan University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowen","family":"Liu","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control, Changsha Hospital for Maternal & Child Health Care Affiliated to Hunan Normal University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zenghui","family":"Mao","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control, Changsha Hospital for Maternal & Child Health Care Affiliated to Hunan Normal University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anji","family":"Chen","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control, Changsha Hospital for Maternal & Child Health Care Affiliated to Hunan Normal University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Liu","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control, Changsha Hospital for Maternal & Child Health Care Affiliated to Hunan Normal University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Xia","sequence":"additional","affiliation":[{"name":"Hunan Provincial Key Laboratory of Regional Hereditary Birth Defects Prevention and Control, Changsha Hospital for Maternal & Child Health Care Affiliated to Hunan Normal University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lifang","family":"Yang","sequence":"additional","affiliation":[{"name":"Cancer Research Institute, Xiangya School of Basic Medicine Sciences, Central South University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1155-6317","authenticated-orcid":true,"given":"Dan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Life Science, College of Biology, Hunan University, Changsha, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"key":"bib1","series-title":"Cell Mol Life Sci","first-page":"2657","article-title":"Insulin-like growth factor 2 mRNA-binding proteins (IGF2BPs): post-transcriptional drivers of cancer progression?","volume":"70","author":"Bell JL","year":"2013","unstructured":"Bell JL\n, \nW\u00e4chter K\n, \nM\u00fchleck B\n, et al.\n 2013 Insulin-like growth factor 2 mRNA-binding proteins (IGF2BPs): post-transcriptional drivers of cancer progression? 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