{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T17:40:46Z","timestamp":1789839646751,"version":"4.0.1"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"2","content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,11,11]]},"abstract":"<jats:sec>\n                    <jats:title>In brief<\/jats:title>\n                    <jats:p>\n                      Steroid hormone secretion by granulosa cells in the ovary is regulated by lipid synthesis and metabolism. This study found that miR-novel-216 affects granulosa cell proliferation and free fatty acid content by regulating\n                      <jats:italic>TPD52<\/jats:italic>\n                      expression, thereby increasing the reproductive hormone secretion and promoting polytocous trait in goats.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      Granulosa cells in the ovaries of livestock are crucial for secreting steroid hormones that regulate follicular development, with lipid synthesis and metabolism playing key roles in this process. The molecular mechanisms behind steroid hormone secretion regulated by fatty acid metabolism in goat granulosa cells have been unclear. Our previous transcriptome analysis of Yunshang black goat ovaries revealed that miR-novel-216, which had lower expression in high-fertility goats, might regulate granulosa cell function. We further investigated the role of miR-novel-216 by isolating and culturing goat granulosa cells\n                      <jats:italic>in vitro<\/jats:italic>\n                      and found that it inhibits cell proliferation, lipid accumulation and progesterone synthesis in goat granulosa cells. The qTar and miRanda analyses predicted\n                      <jats:italic>TPD52<\/jats:italic>\n                      as a target of miR-novel-216, confirmed by dual luciferase and transfection assays. Previous studies have shown that progesterone synthesis in granulosa cells is closely related to free fatty acid composition. We investigated the effect of\n                      <jats:italic>in vitro<\/jats:italic>\n                      construction of\n                      <jats:italic>TPD52<\/jats:italic>\n                      overexpression and interference plasmids on the free fatty acid content of goat granulosa cells using mass spectrometry sequencing. The results showed that the overexpression of\n                      <jats:italic>TPD52<\/jats:italic>\n                      in goat granulosa cells significantly increased the free fatty acid content and promoted granulosa cell proliferation, lipid accumulation and progesterone synthesis, whereas the opposite was true for inhibition of\n                      <jats:italic>TPD52<\/jats:italic>\n                      . It was shown that miR-novel-216 affects granulosa cell proliferation and free fatty acid levels by regulating the expression of TPD52, which increases reproductive hormone secretion and promotes polytocous trait in goats. This provides a foundation for developing breeding strategies to improve goat fertility.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/rep-24-0231","type":"journal-article","created":{"date-parts":[[2024,11,11]],"date-time":"2024-11-11T13:10:42Z","timestamp":1731330642000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":1,"title":["miRNA-novel-216 regulated fatty acid composition and progesterone synthesis in goat granulosa cells by targeting the TPD52"],"prefix":"10.1093","volume":"169","author":[{"given":"Yuhang","family":"Jia","sequence":"first","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China"},{"name":"College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang Agriculture and Forestry University, Hangzhou, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiao","family":"Xu","sequence":"additional","affiliation":[{"name":"Jiangxi\u2019s Provincial Key Laboratory for Enhancing Poultry Genetics, Institution of Biological Technology, Nanchang Normal University, Nanchang, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ziyi","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Runan","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kai","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinghui","family":"Ling","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Anhui Agricultural University, Hefei, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0303-7784","authenticated-orcid":true,"given":"Yufang","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingxing","family":"Chu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"key":"bib1","series-title":"Nat Rev Mol Cell Biol","first-page":"425","article-title":"MicroRNA-mediated regulation of glucose and lipid metabolism","volume":"22","author":"Agbu P","year":"2021","unstructured":"Agbu P & Carthew RW 2021 MicroRNA-mediated regulation of glucose and lipid metabolism. 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