{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T00:38:12Z","timestamp":1788482292204,"version":"build-2803163510"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T00:00:00Z","timestamp":1733961600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2024,12,12]]},"abstract":"<jats:sec>\n                    <jats:title>In brief<\/jats:title>\n                    <jats:p>PI3K-AKT signaling activates steroidogenesis by inducing estradiol and progesterone production, while MEK-ERK1\/2 signaling regulates steroidogenesis by inhibiting estradiol and inducing progesterone production in granulosa cells (GCs). Both pathways are essential for glycolytic and mitochondrial metabolism in these cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      The PI3K-AKT and MEK-ERK1\/2 signaling pathways are integral to fundamental cellular processes, such as proliferation, viability and differentiation. In GCs, these pathways are activated by follicle-stimulating hormone (FSH) and IGF1 through respective receptors. We investigated the comparative transcriptome changes induced by the AKT and ERK (ERK1\/2) pathways using corresponding inhibitors in GCs. GCs isolated from antral follicles showed positive signals for phospho-AKT and phospho-ERK proteins. Treatment of cultured GCs with FSH and IGF1 induced phospho-AKT and phospho-ERK levels. Transcriptome analysis revealed 1436 genes regulated by AKT and 654 genes regulated by the ERK pathway. Among these, 94 genes were commonly downregulated and 11 genes were commonly upregulated in both datasets, while 110 genes were oppositely regulated. Bioinformatics analysis revealed that the inhibition of the PI3K-AKT and MEK-ERK pathways downregulates key reproductive processes and upstream molecules. Notably, AKT inhibition affected FSH, ESRRG and HIF1 pathways, while ERK inhibition impacted CG, FOS, TGF\u03b2, EGR1 and LH pathways. Transcriptome data showed that genes related to estradiol production were inhibited by ERK and induced by the AKT pathway. This was verified by radioimmunoassays, and mRNA and protein analysis of\n                      <jats:italic>CYP19A1<\/jats:italic>\n                      and\n                      <jats:italic>STAR<\/jats:italic>\n                      genes. In addition, transcriptome data suggested the downregulation of glucose metabolism in GCs. Using validation experiments, we confirm that both pathways are essential for glucose uptake, lactate production and mitochondrial activity in GCs. These data provide a resource for informing future research for analyzing various novel candidate genes regulated by the AKT and ERK pathways in GCs and other cell types.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/rep-24-0317","type":"journal-article","created":{"date-parts":[[2024,12,12]],"date-time":"2024-12-12T10:00:25Z","timestamp":1733997625000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":8,"title":["Comparative analysis of PI3K-AKT and MEK-ERK1\/2 signaling-driven molecular changes in granulosa cells"],"prefix":"10.1093","volume":"169","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5856-7777","authenticated-orcid":true,"given":"Vijay Simha","family":"Baddela","sequence":"first","affiliation":[{"name":"Research Institute for Farm Animal Biology (FBN), Dummerstorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marten","family":"Michaelis","sequence":"additional","affiliation":[{"name":"Research Institute for Farm Animal Biology (FBN), Dummerstorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuelian","family":"Tao","sequence":"additional","affiliation":[{"name":"Research Institute for Farm Animal Biology (FBN), Dummerstorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dirk","family":"Koczan","sequence":"additional","affiliation":[{"name":"Institute of Immunology, University of Rostock, Rostock, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Brenmoehl","sequence":"additional","affiliation":[{"name":"Research Institute for Farm Animal Biology (FBN), Dummerstorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Vanselow","sequence":"additional","affiliation":[{"name":"Research Institute for Farm Animal Biology (FBN), Dummerstorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"key":"bib1","series-title":"Endocrinology","first-page":"1795","article-title":"The effect of a null mutation in the follicle-stimulating hormone receptor gene on mouse reproduction","volume":"141","author":"Abel MH","year":"2000","unstructured":"Abel MH, Wootton AN, Wilkins V, et al. 2000 The effect of a null mutation in the follicle-stimulating hormone receptor gene on mouse reproduction. 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