{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T15:07:22Z","timestamp":1788188842212,"version":"build-2803163510"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"4","funder":[{"DOI":"10.13039\/501100022077","name":"Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100022077","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004489","name":"Mitacs","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004489","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,3,10]]},"abstract":"<jats:sec>\n                    <jats:title>In brief<\/jats:title>\n                    <jats:p>Irisin is a muscle and adipose-derived hormone that is secreted in response to negative energy balance in cattle. We show here that irisin reduced follicle growth and theca cell function.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      At the onset of lactation, dairy cattle are anestrous owing mainly to a state of negative energy balance. Adipose tissue is mobilized to meet the energy demands of milk production, and this alters the secretion of adipose-derived hormones, called adipokines. Irisin is a myokine\/adipokine that may play a role in fertility; plasma concentrations increase in cattle postpartum, and irisin decreased progesterone and estradiol secretion from bovine granulosa cells\n                      <jats:italic>in vitro<\/jats:italic>\n                      . To our knowledge, the effects of irisin on bovine theca cell function\n                      <jats:italic>in vitro<\/jats:italic>\n                      and on follicle growth\n                      <jats:italic>in vivo<\/jats:italic>\n                      have not been reported. We hypothesized that irisin negatively affects theca cell function\n                      <jats:italic>in vitro<\/jats:italic>\n                      and causes follicle regression\n                      <jats:italic>in vivo<\/jats:italic>\n                      using well-established bovine models. Under physiological concentrations of insulin (0.2 ng\/mL), irisin did not affect glucose uptake, but decreased testosterone secretion and stimulated PTK2 and MTOR phosphorylation. Inhibiting PTK2 activity abolished the ability of irisin to decrease testosterone secretion. Injection of irisin directly into a growing follicle\n                      <jats:italic>in vivo<\/jats:italic>\n                      caused follicle regression. We conclude that irisin decreases bovine theca cell steroidogenesis through PTK2 signaling, and combined effects on theca and granulosa cells cause follicle regression.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/rep-24-0352","type":"journal-article","created":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T10:20:19Z","timestamp":1741602019000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":5,"title":["Irisin decreases follicle development in cattle and inhibits theca cell steroidogenesis through focal adhesion kinase signaling"],"prefix":"10.1093","volume":"169","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9998-0421","authenticated-orcid":true,"given":"Mathilde","family":"Daudon","sequence":"first","affiliation":[{"name":"CNRS, IFCE, INRAE, Universit\u00e9 de Tours, PRC, Nouzilly, France"},{"name":"Centre de recherche en reproduction et fertilit\u00e9, Facult\u00e9 de m\u00e9decine v\u00e9t\u00e9rinaire, Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Ram\u00e9","sequence":"additional","affiliation":[{"name":"CNRS, IFCE, INRAE, Universit\u00e9 de Tours, PRC, Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcos H","family":"Barreta","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias Rurais, Universidade Federal de Santa Catarina, Curitibanos, Santa Catarina, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfredo Q","family":"Antoniazzi","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Medicina Veterin\u00e1ria, Universidade Federal de Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rio M","family":"Portela","sequence":"additional","affiliation":[{"name":"Programa de P\u00f3s-Gradua\u00e7\u00e3o em Medicina Veterin\u00e1ria, Universidade Federal de Santa Maria (UFSM), Santa Maria, Rio Grande do Sul, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Europa","family":"Meza-Serrano","sequence":"additional","affiliation":[{"name":"Centre de recherche en reproduction et fertilit\u00e9, Facult\u00e9 de m\u00e9decine v\u00e9t\u00e9rinaire, Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8046-7705","authenticated-orcid":true,"given":"Jo\u00eblle","family":"Dupont","sequence":"additional","affiliation":[{"name":"CNRS, IFCE, INRAE, Universit\u00e9 de Tours, PRC, Nouzilly, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6727-8280","authenticated-orcid":true,"given":"Christopher A","family":"Price","sequence":"additional","affiliation":[{"name":"Centre de recherche en reproduction et fertilit\u00e9, Facult\u00e9 de m\u00e9decine v\u00e9t\u00e9rinaire, Universit\u00e9 de Montr\u00e9al, St-Hyacinthe, Quebec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"key":"bib1","series-title":"Domest Anim Endocrinol","article-title":"The myokine irisin: localization and effects in swine late medium and large antral ovarian follicle","volume":"74","author":"Basini G","year":"2021","unstructured":"Basini G\n, \nBussolati S\n, \nIannarelli M\n, et al.\n 2021 The myokine irisin: localization and effects in swine late medium and large antral ovarian follicle. Domest Anim Endocrinol 74 106576. (https:\/\/doi.org\/10.1016\/j.domaniend.2020.106576)"},{"key":"bib2","series-title":"J Endocrinol","first-page":"339","article-title":"Decreased concentration of plasma leptin in periparturient dairy cows is caused by negative energy balance","volume":"171","author":"Block SS","year":"2001","unstructured":"Block SS\n, \nButler WR\n, \nEhrhardt RA\n, et al.\n 2001 Decreased concentration of plasma leptin in periparturient dairy cows is caused by negative energy balance. J Endocrinol 171 339\u2013348. (https:\/\/doi.org\/10.1677\/joe.0.1710339)"},{"key":"bib3","series-title":"Biol Reprod","first-page":"388","article-title":"Consequences of negative energy balance on follicular development and oocyte quality in primiparous sows","volume":"102","author":"Costermans NGJ","year":"2020","unstructured":"Costermans NGJ\n, \nTeerds KJ\n, \nMiddelkoop A\n, et al.\n 2020 Consequences of negative energy balance on follicular development and oocyte quality in primiparous sows. Biol Reprod 102 388\u2013398. (https:\/\/doi.org\/10.1093\/biolre\/ioz175)"},{"key":"bib4","series-title":"Reproduction","first-page":"R1","article-title":"Irisin and the fibronectin type III domain-containing family: structure, signaling and role in female reproduction","volume":"164","author":"Daudon M","year":"2022a","unstructured":"Daudon M\n, \nBigot Y\n, \nDupont J\n, et al.\n 2022a Irisin and the fibronectin type III domain-containing family: structure, signaling and role in female reproduction. Reproduction 164 R1\u2013R9. (https:\/\/doi.org\/10.1530\/rep-22-0037)"},{"key":"bib5","series-title":"Front Vet Sci","article-title":"Impact of fibronectin type III domain-containing family in the changes in metabolic and hormonal profiles during peripartum period in dairy cows","volume":"9","author":"Daudon M","year":"2022b","unstructured":"Daudon M\n, \nRam\u00e9 C\n, \nEstienne A\n, et al.\n 2022b Impact of fibronectin type III domain-containing family in the changes in metabolic and hormonal profiles during peripartum period in dairy cows. Front Vet Sci 9 960778. (https:\/\/doi.org\/10.3389\/fvets.2022.960778)"},{"key":"bib6","series-title":"Reproduction","first-page":"533","article-title":"Irisin modulates glucose metabolism and inhibits steroidogenesis in bovine granulosa cells","volume":"165","author":"Daudon M","year":"2023","unstructured":"Daudon M\n, \nRam\u00e9 C\n, \nPrice C\n, et al.\n 2023 Irisin modulates glucose metabolism and inhibits steroidogenesis in bovine granulosa cells. Reproduction 165 533\u2013542. (https:\/\/doi.org\/10.1530\/rep-22-0404)"},{"key":"bib7","series-title":"Reproduction","article-title":"Exosomal or follicular FNDC3A decreases FOLR1 mRNA abundance and progesterone and lactate synthesis in bovine granulosa cells","volume":"167","author":"Daudon M","year":"2024","unstructured":"Daudon M\n, \nRam\u00e9 C\n, \nPrice CA\n, et al.\n 2024 Exosomal or follicular FNDC3A decreases FOLR1 mRNA abundance and progesterone and lactate synthesis in bovine granulosa cells. Reproduction 167 e230451. (https:\/\/doi.org\/10.1530\/rep-23-0451)"},{"key":"bib8","series-title":"Vet Clin North Am Food Anim Pract","first-page":"299","article-title":"Insulin resistance in dairy cows","volume":"29","author":"De Koster JD","year":"2013","unstructured":"De Koster JD\n & \nOpsomer G\n\n 2013 Insulin resistance in dairy cows. Vet Clin North Am Food Anim Pract 29 299\u2013322. (https:\/\/doi.org\/10.1016\/j.cvfa.2013.04.002)"},{"key":"bib9","series-title":"Reproduction","first-page":"713","article-title":"The role of angiotensin II in the early stages of bovine ovulation","volume":"134","author":"Ferreira R","year":"2007","unstructured":"Ferreira R\n, \nOliveira JF\n, \nFernandes R\n, et al.\n 2007 The role of angiotensin II in the early stages of bovine ovulation. Reproduction 134 713\u2013719. (https:\/\/doi.org\/10.1530\/REP-07-0239)"},{"key":"bib10","series-title":"Endocrinology","first-page":"4957","article-title":"Angiotensin II signaling promotes follicle growth and dominance in cattle","volume":"152","author":"Ferreira R","year":"2011","unstructured":"Ferreira R\n, \nGasperin B\n, \nRovani M\n, et al.\n 2011 Angiotensin II signaling promotes follicle growth and dominance in cattle. Endocrinology 152 4957\u20134965. (https:\/\/doi.org\/10.1210\/en.2011-1146)"},{"key":"bib11","series-title":"Endocrinology","first-page":"5834","article-title":"Adiponectin deficit during the precarious glucose economy of early lactation in dairy cows","volume":"153","author":"Giesy SL","year":"2012","unstructured":"Giesy SL\n, \nYoon B\n, \nCurrie WB\n, et al.\n 2012 Adiponectin deficit during the precarious glucose economy of early lactation in dairy cows. Endocrinology 153 5834\u20135844. (https:\/\/doi.org\/10.1210\/en.2012-1765)"},{"key":"bib12","series-title":"Endocrinology","first-page":"1883","article-title":"Bone morphogenetic proteins (BMP) -4, -6, and -7 potently suppress basal and luteinizing hormone-induced androgen production by bovine theca interna cells in primary culture: could ovarian hyperandrogenic dysfunction be caused by a defect in thecal BMP signaling?","volume":"146","author":"Glister C","year":"2005","unstructured":"Glister C\n, \nRichards SL\n & \nKnight PG\n\n 2005 Bone morphogenetic proteins (BMP) -4, -6, and -7 potently suppress basal and luteinizing hormone-induced androgen production by bovine theca interna cells in primary culture: could ovarian hyperandrogenic dysfunction be caused by a defect in thecal BMP signaling? Endocrinology 146 1883\u20131892. (https:\/\/doi.org\/10.1210\/en.2004-1303)"},{"key":"bib13","series-title":"Reproduction","first-page":"419","article-title":"Effect of dietary-induced increases in circulating insulin concentrations during the early postpartum period on reproductive function in dairy cows","volume":"123","author":"Gong JG","year":"2002","unstructured":"Gong JG\n, \nLee WJ\n, \nGarnsworthy PC\n, et al.\n 2002 Effect of dietary-induced increases in circulating insulin concentrations during the early postpartum period on reproductive function in dairy cows. Reproduction 123 419\u2013427. (https:\/\/doi.org\/10.1530\/rep.0.1230419)"},{"key":"bib14","series-title":"Biol Reprod","first-page":"746","article-title":"The mycotoxin metabolite deepoxy- deoxynivalenol increases apoptosis and decreases steroidogenesis in bovine ovarian theca cells","volume":"97","author":"Guerrero-Netro HM","year":"2017","unstructured":"Guerrero-Netro HM\n, \nEstienne A\n, \nChorfi Y\n, et al.\n 2017 The mycotoxin metabolite deepoxy- deoxynivalenol increases apoptosis and decreases steroidogenesis in bovine ovarian theca cells. Biol Reprod 97 746\u2013757. (https:\/\/doi.org\/10.1093\/biolre\/iox127)"},{"key":"bib15","series-title":"J Biol Chem","article-title":"FNDC5\/irisin facilitates muscle-adipose-bone connectivity through ubiquitination-dependent activation of runt-related transcriptional factors RUNX1\/2","volume":"298","author":"He X","year":"2022","unstructured":"He X\n, \nHua Y\n, \nLi Q\n, et al.\n 2022 FNDC5\/irisin facilitates muscle-adipose-bone connectivity through ubiquitination-dependent activation of runt-related transcriptional factors RUNX1\/2. J Biol Chem 298 101679. (https:\/\/doi.org\/10.1016\/j.jbc.2022.101679)"},{"key":"bib16","series-title":"Cell","first-page":"1756","article-title":"Irisin mediates effects on bone and fat via \u03b1V integrin receptors","volume":"175","author":"Kim H","year":"2018","unstructured":"Kim H\n, \nWrann CD\n, \nJedrychowski M\n, et al.\n 2018 Irisin mediates effects on bone and fat via \u03b1V integrin receptors. Cell 175 1756\u20131768.e17. (https:\/\/doi.org\/10.1016\/j.cell.2018.10.025)"},{"key":"bib17","series-title":"PLoS One","article-title":"Inductions of granulosa cell luteinization and cumulus expansion are dependent on the fibronectin-integrin pathway during ovulation process in mice","volume":"13","author":"Kitasaka H","year":"2018","unstructured":"Kitasaka H\n, \nKawai T\n, \nHoque SAM\n, et al.\n 2018 Inductions of granulosa cell luteinization and cumulus expansion are dependent on the fibronectin-integrin pathway during ovulation process in mice. PLoS One 13 e0192458. (https:\/\/doi.org\/10.1371\/journal.pone.0192458)"},{"key":"bib18","series-title":"Biol Reprod","first-page":"1797","article-title":"Decline in circulating estradiol during the periovulatory period is correlated with decreases in estradiol and androgen, and in messenger RNA for p450 aromatase and p450 17alpha-hydroxylase, in bovine preovulatory follicles","volume":"64","author":"Komar CM","year":"2001","unstructured":"Komar CM\n, \nBerndtson AK\n, \nEvans AC\n, et al.\n 2001 Decline in circulating estradiol during the periovulatory period is correlated with decreases in estradiol and androgen, and in messenger RNA for p450 aromatase and p450 17alpha-hydroxylase, in bovine preovulatory follicles. Biol Reprod 64 1797\u20131805. (https:\/\/doi.org\/10.1095\/biolreprod64.6.1797)"},{"key":"bib19","series-title":"PLoS One","article-title":"Locus characterization and gene expression of bovine FNDC5: is the myokine irisin relevant in cattle?","volume":"9","author":"Komolka K","year":"2014","unstructured":"Komolka K\n, \nAlbrecht E\n, \nSchering L\n, et al.\n 2014 Locus characterization and gene expression of bovine FNDC5: is the myokine irisin relevant in cattle? PLoS One 9 e88060. (https:\/\/doi.org\/10.1371\/journal.pone.0088060)"},{"key":"bib20","series-title":"Mol Cell Endocrinol","first-page":"38","article-title":"Role of adiponectin in regulating ovarian theca and granulosa cell function","volume":"284","author":"Lagaly DV","year":"2008","unstructured":"Lagaly DV\n, \nAad PY\n, \nGrado-Ahuir JA\n, et al.\n 2008 Role of adiponectin in regulating ovarian theca and granulosa cell function. Mol Cell Endocrinol 284 38\u201345. (https:\/\/doi.org\/10.1016\/j.mce.2008.01.007)"},{"key":"bib21","series-title":"Sci Rep","article-title":"Irisin inhibits pancreatic cancer cell growth via the AMPK-mTOR pathway","volume":"8","author":"Liu J","year":"2018","unstructured":"Liu J\n, \nSong N\n, \nHuang Y\n, et al.\n 2018 Irisin inhibits pancreatic cancer cell growth via the AMPK-mTOR pathway. Sci Rep 8 15247. (https:\/\/doi.org\/10.1038\/s41598-018-33229-w)"},{"key":"bib22","series-title":"Endocr Rev","first-page":"436","article-title":"Progress and challenges in the biology of FNDC5 and irisin","volume":"42","author":"Maak S","year":"2021","unstructured":"Maak S\n, \nNorheim F\n, \nDrevon CA\n, et al.\n 2021 Progress and challenges in the biology of FNDC5 and irisin. Endocr Rev 42 436\u2013456. (https:\/\/doi.org\/10.1210\/endrev\/bnab003)"},{"key":"bib23","series-title":"Reprod Biol Endocrinol","first-page":"23","article-title":"Effect of adiponectin on bovine granulosa cell steroidogenesis, oocyte maturation and embryo development","volume":"8","author":"Maillard V","year":"2010","unstructured":"Maillard V\n, \nUzbekova S\n, \nGuignot F\n, et al.\n 2010 Effect of adiponectin on bovine granulosa cell steroidogenesis, oocyte maturation and embryo development. Reprod Biol Endocrinol 8 23. (https:\/\/doi.org\/10.1186\/1477-7827-8-23)"},{"key":"bib24","series-title":"Anim Reprod Sci","article-title":"Regulation and function of leptin during ovarian follicular development in cows","volume":"227","author":"Martins KR","year":"2021","unstructured":"Martins KR\n, \nHaas CS\n, \nRovani MT\n, et al.\n 2021 Regulation and function of leptin during ovarian follicular development in cows. Anim Reprod Sci 227 106689. (https:\/\/doi.org\/10.1016\/j.anireprosci.2021.106689)"},{"key":"bib25","series-title":"Vet J","first-page":"560","article-title":"Elevated non-esterified fatty acids and \u03b2-hydroxybutyrate and their association with transition dairy cow performance","volume":"198","author":"McArt JA","year":"2013","unstructured":"McArt JA\n, \nNydam DV\n, \nOetzel GR\n, et al.\n 2013 Elevated non-esterified fatty acids and \u03b2-hydroxybutyrate and their association with transition dairy cow performance. Vet J 198 560\u2013570. (https:\/\/doi.org\/10.1016\/j.tvjl.2013.08.011)"},{"key":"bib26","series-title":"J Dairy Sci","first-page":"8518","article-title":"Involvement of plasma adipokines in metabolic and reproductive parameters in Holstein dairy cows fed with diets with differing energy levels","volume":"100","author":"Mellouk N","year":"2017","unstructured":"Mellouk N\n, \nRame C\n, \nTouz\u00e9 JL\n, et al.\n 2017 Involvement of plasma adipokines in metabolic and reproductive parameters in Holstein dairy cows fed with diets with differing energy levels. J Dairy Sci 100 8518\u20138533. (https:\/\/doi.org\/10.3168\/jds.2017-12657)"},{"key":"bib27","series-title":"Reprod Biol Endocrinol","first-page":"25","article-title":"Possible involvement of the RARRES2\/CMKLR1-system in metabolic and reproductive parameters in Holstein dairy cows","volume":"17","author":"Mellouk N","year":"2019","unstructured":"Mellouk N\n, \nRam\u00e9 C\n, \nDiot M\n, et al.\n 2019 Possible involvement of the RARRES2\/CMKLR1-system in metabolic and reproductive parameters in Holstein dairy cows. Reprod Biol Endocrinol 17 25. (https:\/\/doi.org\/10.1186\/s12958-019-0467-x)"},{"key":"bib28","series-title":"Cell","first-page":"563","article-title":"CD81 controls beige fat progenitor cell growth and energy balance via FAK signaling","volume":"182","author":"Oguri Y","year":"2020","unstructured":"Oguri Y\n, \nShinoda K\n, \nKim H\n, et al.\n 2020 CD81 controls beige fat progenitor cell growth and energy balance via FAK signaling. Cell 182 563\u2013577.e20. (https:\/\/doi.org\/10.1016\/j.cell.2020.06.021)"},{"key":"bib29","series-title":"Jpn J Vet Res","first-page":"S1","article-title":"Interaction between metabolic challenges and productivity in high yielding dairy cows","volume":"63","author":"Opsomer G","year":"2015","unstructured":"Opsomer G\n\n 2015 Interaction between metabolic challenges and productivity in high yielding dairy cows. Jpn J Vet Res 63(Supp. 1) S1\u2013S14. (https:\/\/doi.org\/10.14943\/jjvr.63.suppl.s1)"},{"key":"bib30","series-title":"Vet Clin Food Anim Pract","first-page":"387","article-title":"Using nonesterified fatty acids and \u03b2-hydroxybutyrate concentrations during the transition period for herd-level monitoring of increased risk of disease and decreased reproductive and milking performance","volume":"29","author":"Ospina PA","year":"2013","unstructured":"Ospina PA\n, \nMcArt JA\n, \nOverton TR\n, et al.\n 2013 Using nonesterified fatty acids and \u03b2-hydroxybutyrate concentrations during the transition period for herd-level monitoring of increased risk of disease and decreased reproductive and milking performance. Vet Clin Food Anim Pract 29 387\u2013412. (https:\/\/doi.org\/10.1016\/j.cvfa.2013.04.003)"},{"key":"bib31","series-title":"Metab Open","article-title":"The effects of irisin and leptin on steroidogenic enzyme gene expression in human granulosa cells: in vitro studies","volume":"17","author":"Poretsky L","year":"2023","unstructured":"Poretsky L\n, \nYeshua A\n, \nCantor T\n, et al.\n 2023 The effects of irisin and leptin on steroidogenic enzyme gene expression in human granulosa cells: in vitro studies. Metab Open 17 100230. (https:\/\/doi.org\/10.1016\/j.metop.2023.100230)"},{"key":"bib32","series-title":"Biol Reprod","first-page":"14","article-title":"The role of fibroblast growth factor-18 in follicular atresia in cattle","volume":"92","author":"Portela VM","year":"2015","unstructured":"Portela VM\n, \nDirandeh E\n, \nGuerrero-Netro HM\n, et al.\n 2015 The role of fibroblast growth factor-18 in follicular atresia in cattle. Biol Reprod 92 14. (https:\/\/doi.org\/10.1095\/biolreprod.114.121376)"},{"key":"bib33","series-title":"J Endocrinol","first-page":"441","article-title":"The susceptibility of granulosa cells to apoptosis is influenced by oestradiol and the cell cycle","volume":"189","author":"Quirk SM","year":"2006","unstructured":"Quirk SM\n, \nCowan RG\n & \nHarman RM\n\n 2006 The susceptibility of granulosa cells to apoptosis is influenced by oestradiol and the cell cycle. J Endocrinol 189 441\u2013453. (https:\/\/doi.org\/10.1677\/joe.1.06549)"},{"key":"bib34","series-title":"Biol Reprod","first-page":"102","article-title":"CHEMERIN (RARRES2) decreases in vitro granulosa cell steroidogenesis and blocks oocyte meiotic progression in bovine species","volume":"90","author":"Reverchon M","year":"2014a","unstructured":"Reverchon M\n, \nBertoldo MJ\n, \nRam\u00e9 C\n, et al.\n 2014a CHEMERIN (RARRES2) decreases in vitro granulosa cell steroidogenesis and blocks oocyte meiotic progression in bovine species. Biol Reprod 90 102. (https:\/\/doi.org\/10.1095\/biolreprod.113.117044)"},{"key":"bib35","series-title":"PLoS One","article-title":"Resistin in dairy cows: plasma concentrations during early lactation, expression and potential role in adipose tissue","volume":"9","author":"Reverchon M","year":"2014b","unstructured":"Reverchon M\n, \nRam\u00e9 C\n, \nCogni\u00e9 J\n, et al.\n 2014b Resistin in dairy cows: plasma concentrations during early lactation, expression and potential role in adipose tissue. PLoS One 9 e93198. (https:\/\/doi.org\/10.1371\/journal.pone.0093198)"},{"key":"bib36","series-title":"Reprod Fertil Dev","first-page":"85","article-title":"Fertility and the transition dairy cow","volume":"30","author":"Roche JR","year":"2017","unstructured":"Roche JR\n, \nBurke CR\n, \nCrookenden MA\n, et al.\n 2017 Fertility and the transition dairy cow. Reprod Fertil Dev 30 85\u2013100. (https:\/\/doi.org\/10.1071\/rd17412)"},{"key":"bib37","series-title":"Cell Tissue Res","first-page":"457","article-title":"Distribution and Y397 phosphorylation of focal adhesion kinase on follicular development in the mouse ovary","volume":"347","author":"Sakurai M","year":"2012","unstructured":"Sakurai M\n, \nOhtake J\n, \nIshikawa T\n, et al.\n 2012 Distribution and Y397 phosphorylation of focal adhesion kinase on follicular development in the mouse ovary. Cell Tissue Res 347 457\u2013465. (https:\/\/doi.org\/10.1007\/s00441-011-1307-2)"},{"key":"bib38","series-title":"Anat Histol Embryol","first-page":"200","article-title":"Identification of stably expressed reference genes for RT-qPCR data normalization in defined localizations of cyclic bovine ovaries","volume":"44","author":"Schoen K","year":"2015","unstructured":"Schoen K\n, \nPlendl J\n, \nGabler C\n, et al.\n 2015 Identification of stably expressed reference genes for RT-qPCR data normalization in defined localizations of cyclic bovine ovaries. Anat Histol Embryol 44 200\u2013211. (https:\/\/doi.org\/10.1111\/ahe.12128)"},{"key":"bib39","series-title":"Drug Discov Today","first-page":"2261","article-title":"The role of irisin in metabolic flexibility: beyond adipose tissue browning","volume":"27","author":"Shen S","year":"2022","unstructured":"Shen S\n, \nLiao Q\n, \nChen X\n, et al.\n 2022 The role of irisin in metabolic flexibility: beyond adipose tissue browning. Drug Discov Today 27 2261\u20132267. (https:\/\/doi.org\/10.1016\/j.drudis.2022.03.019)"},{"key":"bib40","series-title":"Endocrinology","first-page":"3374","article-title":"The adipose obese gene product, leptin: evidence of a direct inhibitory role in ovarian function","volume":"138","author":"Spicer LJ","year":"1997","unstructured":"Spicer LJ\n & \nFrancisco CC\n\n 1997 The adipose obese gene product, leptin: evidence of a direct inhibitory role in ovarian function. Endocrinology 138 3374\u20133379. (https:\/\/doi.org\/10.1210\/endo.138.8.5311)"},{"key":"bib41","series-title":"Biol Reprod","first-page":"207","article-title":"Adipose obese gene product, leptin, inhibits bovine ovarian thecal cell steroidogenesis","volume":"58","author":"Spicer LJ","year":"1998","unstructured":"Spicer LJ\n & \nFrancisco CC\n\n 1998 Adipose obese gene product, leptin, inhibits bovine ovarian thecal cell steroidogenesis. Biol Reprod 58 207\u2013212. (https:\/\/doi.org\/10.1095\/biolreprod58.1.207)"},{"key":"bib42","series-title":"Anim Reprod Sci","first-page":"19","article-title":"Effect of resistin on granulosa and theca cell function in cattle","volume":"124","author":"Spicer LJ","year":"2011","unstructured":"Spicer LJ\n, \nSchreiber NB\n, \nLagaly DV\n, et al.\n 2011 Effect of resistin on granulosa and theca cell function in cattle. Anim Reprod Sci 124 19\u201327. (https:\/\/doi.org\/10.1016\/j.anireprosci.2011.01.005)"},{"key":"bib43","series-title":"J Anim Sci","first-page":"3719","article-title":"Effects of insulin-like growth factor I and insulin on proliferation and on basal and luteinizing hormone-induced steroidogenesis of bovine thecal cells: involvement of glucose and receptors for insulin-like growth factor I and luteinizing hormone","volume":"73","author":"Stewart RE","year":"1995","unstructured":"Stewart RE\n, \nSpicer LJ\n, \nHamilton TD\n, et al.\n 1995 Effects of insulin-like growth factor I and insulin on proliferation and on basal and luteinizing hormone-induced steroidogenesis of bovine thecal cells: involvement of glucose and receptors for insulin-like growth factor I and luteinizing hormone. J Anim Sci 73 3719\u20133731. (https:\/\/doi.org\/10.2527\/1995.73123719x)"},{"key":"bib44","series-title":"Theriogenology","first-page":"659","article-title":"Changes in the gene expression of adiponectin and adiponectin receptors (AdipoR1 and AdipoR2) in ovarian follicular cells of dairy cow at different stages of development","volume":"73","author":"Tabandeh MR","year":"2010","unstructured":"Tabandeh MR\n, \nHosseini A\n, \nSaeb M\n, et al.\n 2010 Changes in the gene expression of adiponectin and adiponectin receptors (AdipoR1 and AdipoR2) in ovarian follicular cells of dairy cow at different stages of development. Theriogenology 73 659\u2013669. (https:\/\/doi.org\/10.1016\/j.theriogenology.2009.11.006)"},{"key":"bib45","series-title":"Mol Cancer Ther","first-page":"2050","article-title":"FAK Inhibition disrupts a \u03b25 integrin signaling axis controlling anchorage-independent ovarian carcinoma growth","volume":"13","author":"Tancioni I","year":"2014","unstructured":"Tancioni I\n, \nUryu S\n, \nSulzmaier FJ\n, et al.\n 2014 FAK Inhibition disrupts a \u03b25 integrin signaling axis controlling anchorage-independent ovarian carcinoma growth. Mol Cancer Ther 13 2050\u20132061. (https:\/\/doi.org\/10.1158\/1535-7163.Mct-13-1063)"},{"key":"bib46","series-title":"Cancer Biol Ther","first-page":"764","article-title":"PND-1186 FAK inhibitor selectively promotes tumor cell apoptosis in three-dimensional environments","volume":"9","author":"Tanjoni I","year":"2010","unstructured":"Tanjoni I\n, \nWalsh C\n, \nUryu S\n, et al.\n 2010 PND-1186 FAK inhibitor selectively promotes tumor cell apoptosis in three-dimensional environments. Cancer Biol Ther 9 764\u2013777. (https:\/\/doi.org\/10.4161\/cbt.9.10.11434)"},{"key":"bib47","series-title":"Theriogenology","first-page":"S232","article-title":"Influence of negative energy balance on cyclicity and fertility in the high producing dairy cow","volume":"68","author":"Wathes DC","year":"2007","unstructured":"Wathes DC\n, \nFenwick M\n, \nCheng Z\n, et al.\n 2007 Influence of negative energy balance on cyclicity and fertility in the high producing dairy cow. Theriogenology 68 (Supplement 1) S232\u2013S241. (https:\/\/doi.org\/10.1016\/j.theriogenology.2007.04.006)"},{"key":"bib48","series-title":"J Ovarian Res","first-page":"151","article-title":"Exercise-induced irisin improves follicular dysfunction by inhibiting IRE1\u03b1-TXNIP\/ROS-NLRP3 pathway in PCOS","volume":"16","author":"Weng Y","year":"2023","unstructured":"Weng Y\n, \nZhang Y\n, \nWang D\n, et al.\n 2023 Exercise-induced irisin improves follicular dysfunction by inhibiting IRE1\u03b1-TXNIP\/ROS-NLRP3 pathway in PCOS. J Ovarian Res 16 151. (https:\/\/doi.org\/10.1186\/s13048-023-01242-x)"},{"key":"bib49","series-title":"Am J Physiol Endocrinol Metab","first-page":"E791","article-title":"Irisin counteracts high glucose and fatty acid-induced cytotoxicity by preserving the AMPK-insulin receptor signaling axis in C2C12 myoblasts","volume":"318","author":"Yano N","year":"2020","unstructured":"Yano N\n, \nZhang L\n, \nWei D\n, et al.\n 2020 Irisin counteracts high glucose and fatty acid-induced cytotoxicity by preserving the AMPK-insulin receptor signaling axis in C2C12 myoblasts. Am J Physiol Endocrinol Metab 318 E791\u2013E805. (https:\/\/doi.org\/10.1152\/ajpendo.00219.2019)"},{"key":"bib50","series-title":"Clin Sci","first-page":"611","article-title":"FNDC5\/Irisin inhibits pathological cardiac hypertrophy","volume":"133","author":"Yu Q","year":"2019","unstructured":"Yu Q\n, \nKou W\n, \nXu X\n, et al.\n 2019 FNDC5\/Irisin inhibits pathological cardiac hypertrophy. Clin Sci 133 611\u2013627. 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