{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,4]],"date-time":"2026-01-04T23:17:38Z","timestamp":1767568658860,"version":"3.48.0"},"reference-count":61,"publisher":"Oxford University Press (OUP)","issue":"6","content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,5,8]]},"abstract":"<jats:sec>\n                    <jats:title>In brief<\/jats:title>\n                    <jats:p>Systemic blockade of Hedgehog signalling reduces sperm motility without knowing whether this effect is direct or indirect on spermatozoa. This study demonstrates the presence of members of the Hedgehog signalling pathway in spermatozoa and the direct effects of activator or inhibitor of this pathway on sperm function.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      The Hedgehog (Hh) signalling pathway controls cellular functions important to organ development and homeostasis. While previously thought to be restricted to non-motile primary cilia, a study indicated that Hh modulates cAMP concentration and beating frequency of airway motile cilia. Since systemic blockade of Hh signalling reduces sperm motility\n                      <jats:italic>in vivo<\/jats:italic>\n                      , we hypothesised that Hh agonists present in the environment surrounding the sperm could directly modulate sperm functions. The presence and localisation of Hh factors, including PATCHED1 receptor, GLI3, and SMOOTHENED, were investigated in spermatozoa from murine, bovine, and human species. Following sperm incubation under capacitating conditions with either Hh agonist (smoothened agonist; SAG) or inhibitor (Cyclopamine), functional sperm parameters were measured, including motility, acrosome integrity, cell viability, intracellular calcium levels, cAMP concentrations, and\n                      <jats:italic>in vitro<\/jats:italic>\n                      fertilisation outcome. Our study demonstrates that Hh signalling pathway regulates sperm motility and acrosome reaction, contributing to fertilisation success. This report proposes new comprehensive signalling mechanisms that control male reproductive functions.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/rep-24-0383","type":"journal-article","created":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T12:56:48Z","timestamp":1746709008000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":0,"title":["Hedgehog signalling in solitary motile cilia: detection and role of hedgehog factors in sperm functions and in vitro fertilization"],"prefix":"10.1093","volume":"169","author":[{"given":"Laura","family":"Girardet","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Department of Obstetrics, Gynecology and Reproduction, Center for Research in Reproduction, Development and Intergenerational Health (CRDSI), Universit\u00e9 Laval, Reproduction, Mother and Youth Health Research Program, CHU de Qu\u00e9bec Research Center (CHUL), Quebec City, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ma\u00edra Bianchi","family":"Rodrigues Alves","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Obstetrics, Gynecology and Reproduction, Center for Research in Reproduction, Development and Intergenerational Health (CRDSI), Universit\u00e9 Laval, Reproduction, Mother and Youth Health Research Program, CHU de Qu\u00e9bec Research Center (CHUL), Quebec City, Quebec, Canada"},{"name":"Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of S\u00e3o Paulo, Pirassununga, SP, Brazil"},{"name":"Department of Pathology, Reproduction and One Healthy, School of Agricultural and Veterinary Sciences, S\u00e3o Paulo State University, Jaboticabal, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Camille","family":"Lavoie-Ouellet","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Obstetrics, Gynecology and Reproduction, Center for Research in Reproduction, Development and Intergenerational Health (CRDSI), Universit\u00e9 Laval, Reproduction, Mother and Youth Health Research Program, CHU de Qu\u00e9bec Research Center (CHUL), Quebec City, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2009-5863","authenticated-orcid":true,"given":"Felipe","family":"Perecin","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, School of Animal Science and Food Engineering, University of S\u00e3o Paulo, Pirassununga, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2053-3958","authenticated-orcid":true,"given":"Pierre","family":"Leclerc","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Obstetrics, Gynecology and Reproduction, Center for Research in Reproduction, Development and Intergenerational Health (CRDSI), Universit\u00e9 Laval, Reproduction, Mother and Youth Health Research Program, CHU de Qu\u00e9bec Research Center (CHUL), Quebec City, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cl\u00e9mence","family":"Belleann\u00e9e","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Department of Obstetrics, Gynecology and Reproduction, Center for Research in Reproduction, Development and Intergenerational Health (CRDSI), Universit\u00e9 Laval, Reproduction, Mother and Youth Health Research Program, CHU de Qu\u00e9bec Research Center (CHUL), Quebec City, Quebec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","reference":[{"key":"bib1","series-title":"J Biol Chem","first-page":"16034","article-title":"Sonic hedgehog enhances calcium oscillations in hippocampal astrocytes","volume":"294","author":"Adachi C","year":"2019","unstructured":"Adachi C\n, \nKakinuma N\n, \nJo SH\n, et al.\n 2019 Sonic hedgehog enhances calcium oscillations in hippocampal astrocytes. 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