{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T22:59:42Z","timestamp":1771455582608,"version":"3.50.1"},"reference-count":10,"publisher":"Oxford University Press (OUP)","issue":"3","content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,1,27]]},"abstract":"\n Wnt7a-Cre<\/jats:italic>\n is a commonly used driver for generating uterine epithelial conditional knockout mice, such as\n epiER\u03b1<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n (\n Esr1<\/jats:italic>\n \n f\/\u2212<\/jats:italic>\n <\/jats:sup>\n Wnt7a<\/jats:italic>\n \n Cre\/+<\/jats:italic>\n <\/jats:sup>\n ) and\n epiPR<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n (\n Pgr<\/jats:italic>\n \n f\/\u2212<\/jats:italic>\n <\/jats:sup>\n Wnt7a<\/jats:italic>\n \n Cre\/+<\/jats:italic>\n <\/jats:sup>\n ). We noticed that\n epiER\u03b1<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n females, but not\n epiPR<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n females, have prolonged plugging latency, which is the duration between continuous cohabitation and detection of the first vaginal plug (a sign of mating). Mating occurs in proestrus and\/or estrus stages of the estrous cycle. Vaginal cytology detected estrous cyclicity in all mice examined, although\n epiER\u03b1<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n mice had leukocyte-dominant vaginal cytology throughout the estrous cycle and their estrous cyclicity appeared less regular. Estrous cyclicity and mating activity are regulated by the hypothalamic\u2013pituitary\u2013ovarian axis, in which kisspeptin plays essential roles. ER\u03b1 and PR are expressed in the rostral periventricular area of the ventricle (RP3V) and arcuate nucleus (ARC) kisspeptin neurons in the hypothalamus. It has been reported that\n Esr1<\/jats:italic>\n \n f\/f<\/jats:italic>\n <\/jats:sup>\n Kiss1-Cre<\/jats:italic>\n mice lack estrous cyclicity, while\n Pgr<\/jats:italic>\n \n f\/f<\/jats:italic>\n <\/jats:sup>\n Kiss1-Cre<\/jats:italic>\n mice have normal estrous cyclicity at two months old, and\n Wnt7a<\/jats:italic>\n is highly expressed in ARC. The prolonged plugging latency in\n epiER\u03b1<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n mice could be contributed by the deletion of ER\u03b1 in\n Wnt7a<\/jats:italic>\n -positive cells in ARC.\n Wnt7a-Cre<\/jats:italic>\n was also used to generate uterine epithelial RhoA-deficient mice,\n epiRhoA<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n (\n RhoA<\/jats:italic>\n \n f\/\u2212<\/jats:italic>\n <\/jats:sup>\n Wnt7a<\/jats:italic>\n \n Cre\/+<\/jats:italic>\n <\/jats:sup>\n ). However, both female and male\n RhoA<\/jats:italic>\n \n f\/\u2212<\/jats:italic>\n <\/jats:sup>\n Wnt7a<\/jats:italic>\n \n Cre\/+<\/jats:italic>\n <\/jats:sup>\n mice had hydrocephalus and died within a few weeks of age. Our observations of increased plugging latency in\n epiER\u03b1<\/jats:italic>\n \u2212\/\u2212<\/jats:sup>\n mice and hydrocephalus in\n RhoA<\/jats:italic>\n \n f\/\u2212<\/jats:italic>\n <\/jats:sup>\n Wnt7a<\/jats:italic>\n \n Cre\/+<\/jats:italic>\n <\/jats:sup>\n mice exemplify unintended neuronal gene deletion using\n Wnt7a-Cre<\/jats:italic>\n for uterine epithelial-specific gene deletion.\n <\/jats:p>","DOI":"10.1530\/rep-24-0447","type":"journal-article","created":{"date-parts":[[2025,1,27]],"date-time":"2025-01-27T10:27:16Z","timestamp":1737973636000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":3,"title":["Increased plugging latency in cycling epiER\u03b1 \u2212\/\u2212 (Esr1 f\/\u2212 Wnt7a Cre\/+ ) mice"],"prefix":"10.1093","volume":"169","author":[{"given":"Jonathan Matthew","family":"Hancock","sequence":"first","affiliation":[{"name":"Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA"},{"name":"Interdisciplinary Toxicology Program, University of Georgia, Athens, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taylor Elijah","family":"Martin","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA"},{"name":"Interdisciplinary Toxicology Program, University of Georgia, Athens, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zidao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA"},{"name":"Interdisciplinary Toxicology Program, University of Georgia, Athens, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jackson Kyle","family":"Sundgren","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1037-9005","authenticated-orcid":true,"given":"Xiaoqin","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Physiology and Pharmacology, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA"},{"name":"Interdisciplinary Toxicology Program, University of Georgia, Athens, Georgia, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","reference":[{"key":"bib1","series-title":"Am J Anat","first-page":"297","article-title":"The oestrous cycle in the mouse","volume":"30","author":"Allen E","year":"1922","unstructured":"Allen E\n\n 1922 The oestrous cycle in the mouse. 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