{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T12:19:06Z","timestamp":1788524346818,"version":"build-2803163510"},"reference-count":62,"publisher":"Oxford University Press (OUP)","issue":"4","funder":[{"DOI":"10.13039\/501100002923","name":"Consejo Nacional de Investigaciones Cient\u00edficas y T\u00e9cnicas","doi-asserted-by":"crossref","award":["PIP 2021\u20132023 #1068"],"award-info":[{"award-number":["PIP 2021\u20132023 #1068"]}],"id":[{"id":"10.13039\/501100002923","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003074","name":"Agencia Nacional de Promoci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"crossref","award":["PICD 2016-0043, PICT 2020-00988"],"award-info":[{"award-number":["PICD 2016-0043, PICT 2020-00988"]}],"id":[{"id":"10.13039\/501100003074","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["RO1RHD106968A"],"award-info":[{"award-number":["RO1RHD106968A"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,2,11]]},"abstract":"<jats:sec>\n                    <jats:title>In brief<\/jats:title>\n                    <jats:p>\n                      The cyclic adenosine monophosphate (cAMP) pathway is essential for maintaining sperm physiology. This study examines a cAMP analog and a phosphodiesterase inhibitor that effectively enhance human sperm motility, thereby improving the efficiency of\n                      <jats:italic>in vitro<\/jats:italic>\n                      sperm selection.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      cAMP plays a central role in sperm physiology. Various cAMP upregulators, both cAMP analogs and phosphodiesterase (PDE) inhibitors, have been used in handling human sperm\n                      <jats:italic>in vitro<\/jats:italic>\n                      , although conflicting results and variable responses among patients have been reported. This study aims to evaluate the ability of two compounds \u2013 Sp-5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole 3\u2032-5\u2032-cyclic monophosphothioate (cBiMPs), a cAMP analog, and TAK-063, a specific PDE10A inhibitor \u2013 to enhance human sperm motility parameters and, consequently, improve sperm preparation procedures. Our results showed that both cBiMPs and TAK-063 significantly enhanced human sperm motility and hyperactivation compared to the control (dimethyl sulphoxide). They also increased protein phosphorylation levels without inducing premature acrosomal exocytosis or DNA fragmentation. The enhancement of sperm motility persisted for 4 h after their removal, surpassing the effects of known cAMP analogs (8-bromo-adenosine-3\u2032, 5\u2032-cAMP (8-Br-cAMP) or dibutyryl cAMP (db-cAMP)) or PDE inhibitors (3-isobutyl-1-methylxanthine (IBMX) or pentoxifylline (PTX)). Furthermore, the presence of cBiMPs or TAK-063 during sperm selection resulted in higher recovery rates in comparison to the control, and these compounds effectively improved sperm motion in both fresh and cryopreserved samples with impaired motility. In conclusion, cBiMPs and TAK-063 exhibit potent and sustained effects on human sperm motility, enhancing the efficiency of sperm preparation techniques. The ability to improve sperm motility holds significant implications for male infertility treatment, facilitating the use of low complexity techniques such as intrauterine insemination or\n                      <jats:italic>in vitro<\/jats:italic>\n                      fertilization, and may also aid in selecting viable testicular sperm for intracytoplasmic sperm injection.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/rep-24-0448","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T06:43:17Z","timestamp":1739256197000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":11,"title":["Potent and sustained enhancement of human sperm motility using novel cyclic AMP upregulators"],"prefix":"10.1093","volume":"169","author":[{"given":"Natalia","family":"Oscoz-Susino","sequence":"first","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2745-8236","authenticated-orcid":true,"given":"Guillermina M","family":"Luque","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florencia","family":"Minotti","sequence":"additional","affiliation":[{"name":"Hospital G. A. Carlos G. Durand, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"Otero","sequence":"additional","affiliation":[{"name":"Hospital G. A. Carlos G. Durand, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariano","family":"Lavolpe","sequence":"additional","affiliation":[{"name":"In Vitro Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Ferrulli","sequence":"additional","affiliation":[{"name":"In Vitro Buenos Aires, Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dario","family":"Krapf","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Rosario (CONICET-UNR). Rosario, Santa Fe, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariano G","family":"Buffone","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7137-3758","authenticated-orcid":true,"given":"Clara I","family":"Mar\u00edn-Briggiler","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"key":"bib1","series-title":"J Reprod Dev","first-page":"171","article-title":"Effects of protein phosphatase inhibitor calyculin a on the postacrosomal protein serine\/threonine phosphorylation state and acrosome reaction in boar spermatozoa incubated with a cAMP analog","volume":"54","author":"Adachi J","year":"2008","unstructured":"Adachi J\n, \nTate S\n, \nMiyake M\n, et al.\n 2008 Effects of protein phosphatase inhibitor calyculin a on the postacrosomal protein serine\/threonine phosphorylation state and acrosome reaction in boar spermatozoa incubated with a cAMP analog. 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The use of recombinant FGF2 to improve motile sperm recovery","volume":"5","author":"Garbarino Az\u00faa DJ","year":"2017","unstructured":"Garbarino Az\u00faa DJ\n, \nSaucedo L\n, \nGiordana S\n, et al.\n 2017 Fibroblast growth factor 2 (FGF2) is present in human spermatozoa and is related with sperm motility. The use of recombinant FGF2 to improve motile sperm recovery. Andrology 5 990\u2013998. (https:\/\/doi.org\/10.1111\/andr.12398)"},{"key":"bib18","series-title":"J Reprod Dev","first-page":"327","article-title":"Calyculin A-sensitive protein phosphatases are involved in maintenance of progressive movement in mouse spermatozoa in vitro by suppression of autophosphorylation of protein kinase A","volume":"55","author":"Goto N","year":"2009","unstructured":"Goto N\n & \nHarayama H\n\n 2009 Calyculin A-sensitive protein phosphatases are involved in maintenance of progressive movement in mouse spermatozoa in vitro by suppression of autophosphorylation of protein kinase A. 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