{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:13:46Z","timestamp":1787022826887,"version":"build-2736575974"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T00:00:00Z","timestamp":1765756800000},"content-version":"vor","delay-in-days":90,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,9,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In brief: Cervical explants alter gene expression and cytokine secretion in response to seminal plasma compared with spermatozoa alone. Results validate the model to study mechanisms behind sperm-immune tolerance in the ovine cervix ex vivo.<\/jats:p>\n                  <jats:p>Abstract: The ovine cervix presents a unique barrier to frozen\u2013thawed ram spermatozoa, reducing pregnancy following cervical artificial insemination (AI). This is hypothesised to be related to a modified sperm molecular profile following seminal plasma (SP) exposure and cryopreservation, altering communication with the female environment. However, the impact of different sperm types on the cervix itself has never been explored, in part due to the difficulty of profiling these in vivo interactions. Here, an ex vivo cell culture model was used to explore changes in the transcriptome of cervical explants following exposure to spermatozoa and SP. Explants were collected from Merino ewes (n = 6) post-mortem and cultured with epididymal, epididymal + SP, frozen and frozen + SP ram spermatozoa, SP only, or a media control for 6 h. Gene expression was profiled by RNA sequencing. Multiplex ELISAs quantified the abundance of cytokines in culture supernatant. Cytokeratin and H&amp;E staining confirmed the presence of cervical epithelial cells in cultured explants. The presence of epididymal sperm caused the differential expression of 18 genes compared with the media-only control, while the addition of SP to epididymal sperm altered 781 genes. Differentially expressed genes were involved in pathways related to inflammation, signalling, and ATP synthesis. SP exposure to frozen sperm led to significantly increased IL1A and reduced TGFB1 production compared with other treatments. Results show an altered cervical immune response in the presence of ram SP and sperm. Further research is required to determine molecular candidates which facilitate immune tolerance and sperm transit through the cervix.<\/jats:p>","DOI":"10.1530\/rep-25-0240","type":"journal-article","created":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T15:41:22Z","timestamp":1758037282000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":5,"title":["Ram sperm and seminal plasma alter immune transcriptome and cytokine secretion in ovine cervical explants\n                    <i>ex vivo<\/i>"],"prefix":"10.1093","volume":"170","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4137-2818","authenticated-orcid":true,"given":"Jessica P","family":"Rickard","sequence":"first","affiliation":[{"name":"School of Life and Environmental Sciences, Faculty of Science, The University of Sydney , Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5428-6591","authenticated-orcid":true,"given":"Sophie","family":"Warr","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Sciences, Faculty of Science, The University of Sydney , Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simon P","family":"de Graaf","sequence":"additional","affiliation":[{"name":"School of Life and Environmental Sciences, Faculty of Science, The University of Sydney , Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4272-9943","authenticated-orcid":true,"given":"Taylor","family":"Pini","sequence":"additional","affiliation":[{"name":"School of Veterinary Science, The University of Queensland , Gatton, Queensland, 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