{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,23]],"date-time":"2026-07-23T02:58:07Z","timestamp":1784775487111,"version":"3.55.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"6","funder":[{"name":"National Key Research and Development Program of China","award":["2022YFC2702702"],"award-info":[{"award-number":["2022YFC2702702"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["32270899, 32470895"],"award-info":[{"award-number":["32270899, 32470895"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"crossref","award":["BK20230004"],"award-info":[{"award-number":["BK20230004"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2025,10,14]]},"abstract":"<jats:sec>\n                    <jats:title>In brief<\/jats:title>\n                    <jats:p>S-palmitoylation is a key protein modification, but the role of ZDHHC5 in male fertility was unknown. This study shows ZDHHC5 loss impairs spermatogenesis, causing male infertility, and identifies ZDHHC5 as a key regulator, offering new insights into infertility mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      S-palmitoylation is a reversible post-translational modification critical for protein localization and function. ZDHHC5, a member of the ZDHHC family of palmitoyltransferases, has well-established roles in cardiovascular, neural, and metabolic systems, but its function in male reproduction remains unclear. To investigate this, we generated\n                      <jats:italic>Zdhhc5<\/jats:italic>\n                      -knockout (KO) mice using CRISPR\/Cas9 and found that male KO mice exhibited complete infertility. Phenotypic analysis revealed significantly reduced testis-to-body weight ratios, decreased sperm count and motility, and increased sperm malformations, including abnormal heads and folded tails. Histological and ultrastructural analyses demonstrated defective spermatogenesis, with aberrant retention of sperm during seminiferous tubule maturation and abnormal membranous structures in sperm. Immunofluorescence showed that ZDHHC5 predominantly localizes to the Golgi apparatus of round spermatids (steps 2\u20138) without affecting Golgi morphology or acrosome biogenesis. While\n                      <jats:italic>in vitro<\/jats:italic>\n                      fertilization with\n                      <jats:italic>Zdhhc5<\/jats:italic>\n                      <jats:sup>\u2212\/\u2212<\/jats:sup>\n                      sperm failed, intracytoplasmic sperm injection (ICSI) successfully rescued male infertility, suggesting functional disruption at the protein modification level rather than genetic material impairment. We also found that ZDHHC5 mediates IFT81 palmitoylation, regulates IFT81 in round spermatids, and links to sperm tail malformations. These findings establish ZDHHC5, a palmitoyltransferase, as a pivotal regulator of spermatogenesis, thereby yielding new insights into the molecular mechanisms underlying male infertility.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1530\/rep-25-0310","type":"journal-article","created":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T16:06:25Z","timestamp":1760457985000},"update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-11","source":"Crossref","is-referenced-by-count":4,"title":["ZDHHC5 deficiency impairs spermatogenesis and causes male infertility in mice"],"prefix":"10.1093","volume":"170","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-1595-9242","authenticated-orcid":true,"given":"Zerui","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yueming","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuya","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinyi","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feixiang","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengwei","family":"Hu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jintao","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine and Offspring Health, Clinical Center of Reproductive Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bianjiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6499-7899","authenticated-orcid":true,"given":"Mingxi","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Reproductive Medicine and Offspring Health, Department of Histology and Embryology, School of Basic Medical Sciences, Nanjing Medical University, Nanjing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"key":"bib1","series-title":"Biochim Biophys Acta","first-page":"2981","article-title":"Protein palmitoylation and subcellular trafficking","volume":"1808","author":"Aicart-Ramos C","year":"2011","unstructured":"Aicart-Ramos C\n, \nValero RA\n & \nRodriguez-Crespo I\n\n 2011 Protein palmitoylation and subcellular trafficking. Biochim Biophys Acta 1808 2981\u20132994. (https:\/\/doi.org\/10.1016\/j.bbamem.2011.07.009)"},{"key":"bib2","series-title":"Mol Membr Biol","first-page":"42","article-title":"Palmitoylation cycles and regulation of protein function (Review)","volume":"26","author":"Baekkeskov S","year":"2009","unstructured":"Baekkeskov S\n & \nKanaani J\n\n 2009 Palmitoylation cycles and regulation of protein function (Review). Mol Membr Biol 26 42\u201354. (https:\/\/doi.org\/10.1080\/09687680802680108)"},{"key":"bib3","series-title":"Nat Neurosci","first-page":"522","article-title":"Palmitoylation of delta-catenin by DHHC5 mediates activity-induced synapse plasticity","volume":"17","author":"Brigidi GS","year":"2014","unstructured":"Brigidi GS\n, \nSun Y\n, \nBeccano-Kelly D\n, et al.\n 2014 Palmitoylation of delta-catenin by DHHC5 mediates activity-induced synapse plasticity. Nat Neurosci 17 522\u2013532. (https:\/\/doi.org\/10.1038\/nn.3657)"},{"key":"bib4","series-title":"Nat Commun","first-page":"8200","article-title":"Activity-regulated trafficking of the palmitoyl-acyl transferase DHHC5","volume":"6","author":"Brigidi GS","year":"2015","unstructured":"Brigidi GS\n, \nSantyr B\n, \nShimell J\n, et al.\n 2015 Activity-regulated trafficking of the palmitoyl-acyl transferase DHHC5. Nat Commun 6 8200. (https:\/\/doi.org\/10.1038\/ncomms9200)"},{"key":"bib5","series-title":"BMC Mol Biol","first-page":"12","article-title":"A potential role for protein palmitoylation and zDHHC16 in DNA damage response","volume":"17","author":"Cao N","year":"2016","unstructured":"Cao N\n, \nLi JK\n, \nRao YQ\n, et al.\n 2016 A potential role for protein palmitoylation and zDHHC16 in DNA damage response. BMC Mol Biol 17 12. (https:\/\/doi.org\/10.1186\/s12867-016-0065-9)"},{"key":"bib6","series-title":"Biophys J","first-page":"826","article-title":"DHHC5 mediates \u03b2-Adrenergic signaling in cardiomyocytes by targeting G\u03b1 proteins","volume":"118","author":"Chen JJ","year":"2020","unstructured":"Chen JJ\n, \nMarsden AN\n, \nScott CA\n, et al.\n 2020 DHHC5 mediates \u03b2-Adrenergic signaling in cardiomyocytes by targeting G\u03b1 proteins. Biophys J 118 826\u2013835. (https:\/\/doi.org\/10.1016\/j.bpj.2019.08.018)"},{"key":"bib7","series-title":"Sci Rep","first-page":"4683","article-title":"Global, site-specific analysis of neuronal protein S-acylation","volume":"7","author":"Collins MO","year":"2017","unstructured":"Collins MO\n, \nWoodley KT\n & \nChoudhary JS\n\n 2017 Global, site-specific analysis of neuronal protein S-acylation. Sci Rep 7 4683. (https:\/\/doi.org\/10.1038\/s41598-017-04580-1)"},{"key":"bib8","series-title":"Nat Rev Mol Cell Biol","first-page":"488","article-title":"Mechanisms and functions of protein S-acylation","volume":"25","author":"Mesquita FS","year":"2024","unstructured":"Mesquita FS\n, \nAbrami L\n, \nLinder ME\n, et al.\n 2024 Mechanisms and functions of protein S-acylation. Nat Rev Mol Cell Biol 25 488\u2013509. (https:\/\/doi.org\/10.1038\/s41580-024-00700-8)"},{"key":"bib9","series-title":"Neuron","first-page":"987","article-title":"Identification of PSD-95 palmitoylating enzymes","volume":"44","author":"Fukata M","year":"2004","unstructured":"Fukata M\n, \nFukata Y\n, \nAdesnik H\n, et al.\n 2004 Identification of PSD-95 palmitoylating enzymes. Neuron 44 987\u2013996. (https:\/\/doi.org\/10.1016\/j.neuron.2004.12.005)"},{"key":"bib10","series-title":"Reprod Sci","first-page":"2299","article-title":"Proteome-wide identification of palmitoylated proteins in mouse testis","volume":"29","author":"Gao J","year":"2022","unstructured":"Gao J\n, \nLi W\n, \nZhang Z\n, et al.\n 2022 Proteome-wide identification of palmitoylated proteins in mouse testis. Reprod Sci 29 2299\u20132309. (https:\/\/doi.org\/10.1007\/s43032-022-00919-w)"},{"key":"bib11","series-title":"Nature","first-page":"459","article-title":"A SARS-CoV-2 protein interaction map reveals targets for drug repurposing","volume":"583","author":"Gordon DE","year":"2020","unstructured":"Gordon DE\n, \nJang GM\n, \nBouhaddou M\n, et al.\n 2020 A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Nature 583 459\u2013468. (https:\/\/doi.org\/10.1038\/s41586-020-2286-9)"},{"key":"bib12","series-title":"Nat Commun","first-page":"4765","article-title":"CD36 facilitates fatty acid uptake by dynamic palmitoylation-regulated endocytosis","volume":"11","author":"Hao JW","year":"2020","unstructured":"Hao JW\n, \nWang J\n, \nGuo H\n, et al.\n 2020 CD36 facilitates fatty acid uptake by dynamic palmitoylation-regulated endocytosis. Nat Commun 11 4765. (https:\/\/doi.org\/10.1038\/s41467-020-18565-8)"},{"key":"bib13","series-title":"ELife","article-title":"Massive endocytosis triggered by surface membrane palmitoylation under mitochondrial control in BHK fibroblasts","volume":"2","author":"Hilgemann DW","year":"2013","unstructured":"Hilgemann DW\n, \nFine M\n, \nLinder ME\n, et al.\n 2013 Massive endocytosis triggered by surface membrane palmitoylation under mitochondrial control in BHK fibroblasts. ELife 2 e01293. (https:\/\/doi.org\/10.7554\/elife.01293)"},{"key":"bib14","series-title":"Proc Natl Acad Sci U S A","article-title":"TEX38 localizes ZDHHC19 to the plasma membrane and regulates sperm head morphogenesis in mice","volume":"122","author":"Kaneda Y","year":"2025","unstructured":"Kaneda Y\n, \nLu Y\n, \nSun J\n, et al.\n 2025 TEX38 localizes ZDHHC19 to the plasma membrane and regulates sperm head morphogenesis in mice. Proc Natl Acad Sci U S A 122 e2417943122. (https:\/\/doi.org\/10.1073\/pnas.2417943122)"},{"key":"bib15","series-title":"Eur J Cell Biol","first-page":"107","article-title":"Human DHHC proteins: a spotlight on the hidden player of palmitoylation","volume":"91","author":"Korycka J","year":"2012","unstructured":"Korycka J\n, \n\u0141ach A\n, \nHeger E\n, et al.\n 2012 Human DHHC proteins: a spotlight on the hidden player of palmitoylation. Eur J Cell Biol 91 107\u2013117. (https:\/\/doi.org\/10.1016\/j.ejcb.2011.09.013)"},{"key":"bib16","series-title":"J Biol Chem","first-page":"13022","article-title":"DHHC5 interacts with PDZ domain 3 of post-synaptic density-95 (PSD-95) protein and plays a role in learning and memory","volume":"285","author":"Li Y","year":"2010","unstructured":"Li Y\n, \nHu J\n, \nHofer K\n, et al.\n 2010 DHHC5 interacts with PDZ domain 3 of post-synaptic density-95 (PSD-95) protein and plays a role in learning and memory. J Biol Chem 285 13022\u201313031. (https:\/\/doi.org\/10.1074\/jbc.m109.079426)"},{"key":"bib17","series-title":"J Med Genet","first-page":"89","article-title":"Biallelic mutations in CFAP65 cause male infertility with multiple morphological abnormalities of the sperm flagella in humans and mice","volume":"57","author":"Li W","year":"2020","unstructured":"Li W\n, \nWu H\n, \nLi F\n, et al.\n 2020 Biallelic mutations in CFAP65 cause male infertility with multiple morphological abnormalities of the sperm flagella in humans and mice. J Med Genet 57 89\u201395. (https:\/\/doi.org\/10.1136\/jmedgenet-2019-106344)"},{"key":"bib18","series-title":"ELife","article-title":"Massive palmitoylation-dependent endocytosis during reoxygenation of anoxic cardiac muscle","volume":"2","author":"Lin MJ","year":"2013","unstructured":"Lin MJ\n, \nFine M\n, \nLu JY\n, et al.\n 2013 Massive palmitoylation-dependent endocytosis during reoxygenation of anoxic cardiac muscle. ELife 2 e01295. (https:\/\/doi.org\/10.7554\/elife.01295)"},{"key":"bib19","series-title":"Mol Hum Reprod","first-page":"463","article-title":"SHCBP1L, a conserved protein in mammals, is predominantly expressed in male germ cells and maintains spindle stability during meiosis in testis","volume":"20","author":"Liu M","year":"2014","unstructured":"Liu M\n, \nShi X\n, \nBi Y\n, et al.\n 2014 SHCBP1L, a conserved protein in mammals, is predominantly expressed in male germ cells and maintains spindle stability during meiosis in testis. Mol Hum Reprod 20 463\u2013475. (https:\/\/doi.org\/10.1093\/molehr\/gau014)"},{"key":"bib20","series-title":"Science","first-page":"460","article-title":"Palmitoylation of NOD1 and NOD2 is required for bacterial sensing","volume":"366","author":"Lu Y","year":"2019","unstructured":"Lu Y\n, \nZheng Y\n, \nCoyaud \u00c9\n, et al.\n 2019 Palmitoylation of NOD1 and NOD2 is required for bacterial sensing. Science 366 460\u2013467. (https:\/\/doi.org\/10.1126\/science.aau6391)"},{"key":"bib21","series-title":"J Lipid Res","first-page":"1118","article-title":"Protein palmitoylation by a family of DHHC protein S-acyltransferases","volume":"47","author":"Mitchell DA","year":"2006","unstructured":"Mitchell DA\n, \nVasudevan A\n, \nLinder ME\n, et al.\n 2006 Protein palmitoylation by a family of DHHC protein S-acyltransferases. J Lipid Res 47 1118\u20131127. (https:\/\/doi.org\/10.1194\/jlr.r600007-jlr200)"},{"key":"bib22","series-title":"Commun Biol","first-page":"411","article-title":"Control of protein palmitoylation by regulating substrate recruitment to a zDHHC-protein acyltransferase","volume":"3","author":"Plain F","year":"2020","unstructured":"Plain F\n, \nHowie J\n, \nKennedy J\n, et al.\n 2020 Control of protein palmitoylation by regulating substrate recruitment to a zDHHC-protein acyltransferase. Commun Biol 3 411. (https:\/\/doi.org\/10.1038\/s42003-020-01145-3)"},{"key":"bib23","series-title":"Am J Physiol Cell Physiol","first-page":"C1092","article-title":"The essential role of intraflagellar transport protein IFT81 in male mice spermiogenesis and fertility","volume":"318","author":"Qu W","year":"2020","unstructured":"Qu W\n, \nYuan S\n, \nQuan C\n, et al.\n 2020 The essential role of intraflagellar transport protein IFT81 in male mice spermiogenesis and fertility. Am J Physiol Cell Physiol 318 C1092\u2013C1106. (https:\/\/doi.org\/10.1152\/ajpcell.00450.2019)"},{"key":"bib24","series-title":"Methods","first-page":"191","article-title":"Use of analogs and inhibitors to study the functional significance of protein palmitoylation","volume":"40","author":"Resh MD","year":"2006","unstructured":"Resh MD\n\n 2006 Use of analogs and inhibitors to study the functional significance of protein palmitoylation. Methods 40 191\u2013197. (https:\/\/doi.org\/10.1016\/j.ymeth.2006.04.013)"},{"key":"bib25","series-title":"Methods","first-page":"135","article-title":"Proteomic identification of palmitoylated proteins","volume":"40","author":"Roth AF","year":"2006","unstructured":"Roth AF\n, \nWan J\n, \nGreen WN\n, et al.\n 2006 Proteomic identification of palmitoylated proteins. Methods 40 135\u2013142. (https:\/\/doi.org\/10.1016\/j.ymeth.2006.05.026)"},{"key":"bib26","series-title":"Proc Natl Acad Sci U S A","first-page":"1279","article-title":"Anthrax toxin requires ZDHHC5-mediated palmitoylation of its surface-processing host enzymes","volume":"116","author":"Sergeeva OA","year":"2019","unstructured":"Sergeeva OA\n & \nvan der Goot FG\n\n 2019 Anthrax toxin requires ZDHHC5-mediated palmitoylation of its surface-processing host enzymes. Proc Natl Acad Sci U S A 116 1279\u20131288. (https:\/\/doi.org\/10.1073\/pnas.1812588116)"},{"key":"bib27","series-title":"Asian J Androl","first-page":"345","article-title":"Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice","volume":"21","author":"Shen C","year":"2019","unstructured":"Shen C\n, \nYu J\n, \nZhang X\n, et al.\n 2019 Strawberry Notch 1 (SBNO1) promotes proliferation of spermatogonial stem cells via the noncanonical Wnt pathway in mice. Asian J Androl 21 345\u2013350. (https:\/\/doi.org\/10.4103\/aja.aja_65_18)"},{"key":"bib28","series-title":"Neuron","first-page":"482","article-title":"Palmitoylation by DHHC5\/8 targets GRIP1 to dendritic endosomes to regulate AMPA-R trafficking","volume":"73","author":"Thomas GM","year":"2012","unstructured":"Thomas GM\n, \nHayashi T\n, \nChiu SL\n, et al.\n 2012 Palmitoylation by DHHC5\/8 targets GRIP1 to dendritic endosomes to regulate AMPA-R trafficking. Neuron 73 482\u2013496. (https:\/\/doi.org\/10.1016\/j.neuron.2011.11.021)"},{"key":"bib29","series-title":"J Biol Chem","first-page":"36020","article-title":"The inhibitory effect of phospholemman on the sodium pump requires its palmitoylation","volume":"286","author":"Tulloch LB","year":"2011","unstructured":"Tulloch LB\n, \nHowie J\n, \nWypijewski KJ\n, et al.\n 2011 The inhibitory effect of phospholemman on the sodium pump requires its palmitoylation. J Biol Chem 286 36020\u201336031. (https:\/\/doi.org\/10.1074\/jbc.m111.282145)"},{"key":"bib30","series-title":"Nat Protoc","first-page":"1573","article-title":"Palmitoylated proteins: purification and identification","volume":"2","author":"Wan J","year":"2007","unstructured":"Wan J\n, \nRoth AF\n, \nBailey AO\n, et al.\n 2007 Palmitoylated proteins: purification and identification. Nat Protoc 2 1573\u20131584. (https:\/\/doi.org\/10.1038\/nprot.2007.225)"},{"key":"bib31","series-title":"Int J Mol Sci","first-page":"8894","article-title":"ZDHHC19 is dispensable for spermatogenesis, but is essential for sperm functions in mice","volume":"22","author":"Wang S","year":"2021","unstructured":"Wang S\n, \nQiao H\n, \nWang P\n, et al.\n 2021 ZDHHC19 is dispensable for spermatogenesis, but is essential for sperm functions in mice. Int J Mol Sci 22 8894. (https:\/\/doi.org\/10.3390\/ijms22168894)"},{"key":"bib32","series-title":"Theranostics","first-page":"988","article-title":"Palmitoylation of PKC\u03b4 by ZDHHC5 in hypothalamic microglia presents as a therapeutic target for fatty liver disease","volume":"14","author":"Wang YH","year":"2024","unstructured":"Wang YH\n, \nChen X\n, \nBai YZ\n, et al.\n 2024 Palmitoylation of PKC\u03b4 by ZDHHC5 in hypothalamic microglia presents as a therapeutic target for fatty liver disease. Theranostics 14 988\u20131009. (https:\/\/doi.org\/10.7150\/thno.89602)"},{"key":"bib33","series-title":"EMBO Rep","article-title":"S-acylated Golga7b stabilises DHHC5 at the plasma membrane to regulate cell adhesion","volume":"20","author":"Woodley KT","year":"2019","unstructured":"Woodley KT\n & \nCollins MO\n\n 2019 S-acylated Golga7b stabilises DHHC5 at the plasma membrane to regulate cell adhesion. EMBO Rep 20 e47472. (https:\/\/doi.org\/10.15252\/embr.201847472)"},{"key":"bib34","series-title":"FEBS J","first-page":"6623","article-title":"Regulation and function of the palmitoyl\u2010acyltransferase ZDHHC5","volume":"288","author":"Woodley KT","year":"2021","unstructured":"Woodley KT\n & \nCollins MO\n\n 2021 Regulation and function of the palmitoyl\u2010acyltransferase ZDHHC5. FEBS J 288 6623\u20136634. (https:\/\/doi.org\/10.1111\/febs.15709)"},{"key":"bib35","series-title":"PeerJ","article-title":"Retinoic acid induced protein 14 (Rai14) is dispensable for mouse spermatogenesis","volume":"9","author":"Wu Y","year":"2021","unstructured":"Wu Y\n, \nWang T\n, \nZhao Z\n, et al.\n 2021 Retinoic acid induced protein 14 (Rai14) is dispensable for mouse spermatogenesis. PeerJ 9 e10847. (https:\/\/doi.org\/10.7717\/peerj.10847)"},{"key":"bib36","series-title":"Biol Reprod","first-page":"477","article-title":"ZDHHC19 localizes to the cell membrane of spermatids and is involved in spermatogenesis","volume":"106","author":"Wu Y","year":"2022","unstructured":"Wu Y\n, \nZhang X\n, \nZhang X\n, et al.\n 2022 ZDHHC19 localizes to the cell membrane of spermatids and is involved in spermatogenesis. Biol Reprod 106 477\u2013486. (https:\/\/doi.org\/10.1093\/biolre\/ioab224)"},{"key":"bib37","series-title":"Mol Cell Proteomics","first-page":"54","article-title":"Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes","volume":"9","author":"Yang W","year":"2010","unstructured":"Yang W\n, \nDi Vizio D\n, \nKirchner M\n, et al.\n 2010 Proteome scale characterization of human S-acylated proteins in lipid raft-enriched and non-raft membranes. Mol Cell Proteomics 9 54\u201370. (https:\/\/doi.org\/10.1074\/mcp.m800448-mcp200)"},{"key":"bib38","series-title":"Reproduction","first-page":"59","article-title":"Cellular nucleic acid-binding protein is vital to testis development and spermatogenesis in mice","volume":"156","author":"Zheng B","year":"2018","unstructured":"Zheng B\n, \nYu J\n, \nGuo Y\n, et al.\n 2018 Cellular nucleic acid-binding protein is vital to testis development and spermatogenesis in mice. Reproduction 156 59\u201369. (https:\/\/doi.org\/10.1530\/rep-17-0666)"},{"key":"bib39","series-title":"Mol Cell","first-page":"4570","article-title":"ZDHHC5-mediated NLRP3 palmitoylation promotes NLRP3-NEK7 interaction and inflammasome activation","volume":"83","author":"Zheng S","year":"2023","unstructured":"Zheng S\n, \nQue X\n, \nWang S\n, et al.\n 2023 ZDHHC5-mediated NLRP3 palmitoylation promotes NLRP3-NEK7 interaction and inflammasome activation. Mol Cell 83 4570\u20134585.e7. 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