{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T14:22:05Z","timestamp":1772893325586,"version":"3.50.1"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2005,7,1]],"date-time":"2005-07-01T00:00:00Z","timestamp":1120176000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2005,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The effectiveness of \u03b22-agonists in preterm delivery is reduced by several factors. The aim of this study was to determine the influence of late pregnancy in the uterus-relaxing effect of terbutaline in the rat in vitro.<\/jats:p>\n                  <jats:p>Rat uterine tissues from late pregnancy (days 15, 18, 20 and 22) were used. In vitro electrical field-stimulation (EFS) was used to evoke contractions. The radioligand-binding technique, reverse transcription-polymerase chain reaction and radioimmunoassay technique were used to determine the \u03b2-adrenergic receptor density and mRNA level and the plasma sex hormone level, respectively. The activated G-protein level of the \u03b2-adrenergic receptors was investigated by a radiolabelled GTP binding assay.<\/jats:p>\n                  <jats:p>EFS-induced contractions were inhibited by terbutaline. This effect decreased towards term with respect to both the EC50 and maximal inhibition values. A drop in plasma progesterone level was also detected. Binding studies revealed an increase in \u03b2-adrenergic receptor number on the last day of pregnancy, which correlated with the change in receptor mRNA level. The G-protein-activating effect of terbutaline decreased continuously between days 15 and 20. Surprisingly, terbutaline decreased the G-protein activation to below the basal level on day 22. However, progesterone pretreatment set back the uterine action of terbutaline, increased the density of the \u03b22-adrenergic receptors and their mRNA level and increased the G-protein-activating property of terbutaline.<\/jats:p>\n                  <jats:p>These data provide evidence of a pregnancy-induced decrease in activated G-protein level after \u03b22-agonist stimulation. The decrease in plasma progesterone level has a crucial role in this process. The effects of \u03b22-adrenergic receptor agonists in tocolytic therapy may possibly be potentiated with progesterone.<\/jats:p>","DOI":"10.1530\/rep.1.00490","type":"journal-article","created":{"date-parts":[[2005,6,28]],"date-time":"2005-06-28T14:33:58Z","timestamp":1119969238000},"page":"113-122","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":19,"title":["Pregnancy-induced decrease in the relaxant effect of terbutaline in the late-pregnant rat myometrium: role of G-protein activation and progesterone"],"prefix":"10.1093","volume":"130","author":[{"given":"Ro\u0301bert","family":"Ga\u0301spa\u0301r","sequence":"first","affiliation":[{"name":"Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, H-6720 Szeged, Eo\u0308tvo\u0308s u. 6, 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