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In this study, we examined the characteristics of carnitine transport in primary-cultured rat Sertoli cells, which constitute a part of the blood\u2013testis barrier. Uptake of [3H]carnitine (11.4 nM) from the basal side of Sertoli cells was Na+-dependent and was significantly decreased in the presence of 10 \u03bcM (48.0 \u00b1 7.4% of control) or 100 \u03bcM unlabeled carnitine (14.6 \u00b1 5.7% of control). Furthermore, the uptake was significantly inhibited in the presence of 100 \u03bcM acetyl-L-carnitine, 100 \u03bcM gamma-butyrobetaine or 500 \u03bcM quinidine. In RT-PCR analysis, the high-affinity carnitine transporter OCTN2 was detected in rat whole testis tissue and primary-cultured Sertoli cells. In contrast, the low-affinity carnitine transporter ATB0,+ was detected in rat whole testis tissue, but not in primary cultured Sertoli cells. These results demonstrate that OCTN2 mediates carnitine supply to Sertoli cells from the circulation.<\/jats:p>","DOI":"10.1530\/rep.1.00507","type":"journal-article","created":{"date-parts":[[2005,5,27]],"date-time":"2005-05-27T15:07:43Z","timestamp":1117206463000},"page":"729-736","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":28,"title":["OCTN2-mediated transport of carnitine in isolated Sertoli cells"],"prefix":"10.1093","volume":"129","author":[{"given":"Daisuke","family":"Kobayashi","sequence":"first","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamasaki, Noda, Chiba 278-8510, Japan"},{"name":"Faculty of Pharmaceutical Sciences, Kanazawa University, Kakuma, Kanazawa 920-1192, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihiko","family":"Goto","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamasaki, Noda, Chiba 278-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoji","family":"Maeda","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamasaki, Noda, Chiba 278-8510, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun-ichi","family":"Nezu","sequence":"additional","affiliation":[{"name":"Chugai Pharmaceutical Co. 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