{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T00:54:16Z","timestamp":1792025656699,"version":"4.4.0"},"reference-count":64,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2005,4,1]],"date-time":"2005-04-01T00:00:00Z","timestamp":1112313600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":["rep.bioscientifica.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2005,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>An increase in scrotal temperature can lead to the production of poor quality spermatozoa and infertility. In the present study we have used mice to examine the impact of mild, scrotal heat stress (42 \u00b0C for 30 min) on numbers of spermatozoa as well as on the integrity of their DNA. Spermatozoa recovered from the epididymides hours (1 to 24) or days (7 to 32) after treatment were analysed using COMET and sperm chromatin structure (SCSA) assays. The treatment induced a stress response in both the testis and the epididymis that was associated with reduced expression of the cold inducible RNA binding protein (Cirp) and an increase in germ cell apoptosis (Apotag positive cells). Although spermatozoa present in the epididymis at the time of heating contained correctly packaged DNA, its integrity was compromised by heat stress. In addition, although some germ cells, which were present within the testis at the time of heat stress, were removed by apoptosis, many germ cells completed their development and were recovered as motile spermatozoa with damaged DNA. In conclusion, these data demonstrate that scrotal heat stress can compromise the DNA integrity of spermatozoa and this may have clinical implications for patients undergoing IVF and intra-cytoplasmic sperm injection (ICSI).<\/jats:p>","DOI":"10.1530\/rep.1.00531","type":"journal-article","created":{"date-parts":[[2005,3,29]],"date-time":"2005-03-29T13:29:40Z","timestamp":1112102980000},"page":"505-514","update-policy":"https:\/\/doi.org\/10.1530\/crossmarkpolicy-1","source":"Crossref","is-referenced-by-count":203,"title":["Impact of a mild scrotal heat stress on DNA integrity in murine spermatozoa"],"prefix":"10.1093","volume":"129","author":[{"given":"Stephen","family":"Banks","sequence":"first","affiliation":[{"name":"MRC Human Reproductive Sciences Unit, The Chancellor\u2019s Building, 49 Little France Crescent, Edinburgh, EH16 4SB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sasha A","family":"King","sequence":"additional","affiliation":[{"name":"MRC Human Reproductive Sciences Unit, The Chancellor\u2019s Building, 49 Little France Crescent, Edinburgh, EH16 4SB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D Stewart","family":"Irvine","sequence":"additional","affiliation":[{"name":"MRC Human Reproductive Sciences Unit, The Chancellor\u2019s Building, 49 Little France Crescent, Edinburgh, EH16 4SB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippa T K","family":"Saunders","sequence":"additional","affiliation":[{"name":"MRC Human Reproductive Sciences Unit, The Chancellor\u2019s Building, 49 Little France Crescent, Edinburgh, EH16 4SB, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2005,4,1]]},"reference":[{"key":"2026010713013337300_AGUILAR-MAHECHA-ETAL-2001","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1095\/biolreprod65.1.119","volume":"65","year":"2001","journal-title":"Biology of Reproduction"},{"key":"2026010713013337300_AITKEN-AND-KRAUSZ-2001","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1530\/rep.0.1220497","volume":"122","year":"2001","journal-title":"Reproduction"},{"key":"2026010713013337300_AITKEN-ETAL-1989","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1095\/biolreprod41.1.183","volume":"41","year":"1989","journal-title":"Biology of Reproduction"},{"key":"2026010713013337300_AITKEN-ETAL-1998","doi-asserted-by":"crossref","first-page":"1037","DOI":"10.1095\/biolreprod59.5.1037","volume":"59","year":"1998","journal-title":"Biology of Reproduction"},{"key":"2026010713013337300_BARROSO-ETAL-2000","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1093\/humrep\/15.6.1338","volume":"15","year":"2000","journal-title":"Human Reproduction"},{"key":"2026010713013337300_BAUCHE-ETAL-1994","doi-asserted-by":"crossref","first-page":"392","DOI":"10.1016\/0014-5793(94)00709-8","volume":"349","year":"1994","journal-title":"FEBS letters"},{"key":"2026010713013337300_BEDFORD-1991","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1007\/978-1-4684-5913-5_3","volume":"286","year":"1991","journal-title":"Advances in Experimental Medicine and Biology"},{"key":"2026010713013337300_BEDFORD-1994","doi-asserted-by":"crossref","first-page":"2187","DOI":"10.1093\/oxfordjournals.humrep.a138416","volume":"9","year":"1994","journal-title":"Human Reproduction"},{"key":"2026010713013337300_BIGGERS-ETAL-1971","year":"1971"},{"key":"2026010713013337300_CAMPBELL-AND-IRVINE-2000","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1258\/0007142001903427","volume":"56","year":"2000","journal-title":"British Medical Bulletin"},{"key":"2026010713013337300_COLLINS-AND-LACY-1969","first-page":"17","volume":"172","year":"1969","journal-title":"Proceedings of the Royal Society of London. 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